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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei589 – 5891ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-648-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:AF_0633
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
ProteomesiUP000002199 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10181018Isoleucine--tRNA ligasePRO_0000098575Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi224325.AF0633.

Structurei

3D structure databases

ProteinModelPortaliO29622.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi43 – 5311"HIGH" regionAdd
BLAST
Motifi586 – 5905"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiVLGDWDN.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O29622-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPAKYSPKD VESEIFKFWE DNDIYRKVKE KGGRKFYFVD GPPYTTGRIH
60 70 80 90 100
LGTAWNKVLK DTILRYKRMM GFAPTDTPGW DMHGLPIEVK VEQELGFRTK
110 120 130 140 150
RDIESFGIDK FIERCMNYAL ANKDAMTEQF KSLAVWMDWE NPYMTIKAEY
160 170 180 190 200
MNAAWFAIKR AHERGLLERK KMVVNWCHRC ETALADAEVE YWDEEDPSIY
210 220 230 240 250
VKFPVKGEKD TYIVIWTTTP WTLPANMAVA VHPSLEYAKF RAVKDGKVEY
260 270 280 290 300
LILAKELADS VLGKGDYDSW EVVETYLGED LEGLEYEHPL ADEVPLQKNW
310 320 330 340 350
KHQVFFADFV TAENTGCVHI APGHGVEDYE LGVEKGLEVF NPVDDRGVYT
360 370 380 390 400
EEAGKYAGKH VKEANDDIID DLYRKDLLLA EERIVHRYGH CWRCKTPIIY
410 420 430 440 450
RATEQWFIKI SELKDEMLEE IDKVMWIPEW AGSARFKDWV SNAKDWCISR
460 470 480 490 500
QRYWGIPIPV WICEKCGEMK VVGSINEIEW ENDLDLHRPK IDAVTFSCQC
510 520 530 540 550
GGVMRRVPDV FDVWFDSGVA SWGSIAYPLR KDKFEELWPA DFITEGHDQT
560 570 580 590 600
RGWFYSQLGT SVVCFDKAPY KAVLMHGFTL DEQGRKMSKS LGNVVEPEEV
610 620 630 640 650
VGQIGVDGFR LYVLYSAPWE DLRFSWEEAR NINRMLNVVW NAVRFAHTYM
660 670 680 690 700
SLDNYSFGEK GELKIEDRWI LSRLESFIKE ANEAMEGYQV HRVVRAFFDF
710 720 730 740 750
FVEDFSRWYI QIIRPRVWEE RDSPSKLAAY YTMFRVIDRS LRAIAPFAPL
760 770 780 790 800
IAEWFYQHVV KEFREGEESI FMEEYSTAEV EMIDGELEAA MKVAKEIVEA
810 820 830 840 850
AANARNKAKR KLRWPLRELV IESGSEKVRK AVEMLEETIL SQCNVKQVRV
860 870 880 890 900
VDSFEKEIEI KPNYKYIGPL LKEKAGEFAK YVASLKEIPE KLVFDGVELD
910 920 930 940 950
PKQAIVVDYR LPEGYEYAEF SGGVVYIYKE LDDELVREAF AREVIRRIQE
960 970 980 990 1000
MRKELDLDVE EFIETTVEMD AELVKGWEDY IKSETRSQKL VFGKAEGYVR
1010
EWNIEGKKVK IGIKRLRG
Length:1,018
Mass (Da):118,885
Last modified:January 1, 1998 - v1
Checksum:i58C8BBE472FF1D0F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB90608.1.
PIRiA69329.
RefSeqiNP_069467.1. NC_000917.1.
WP_010878137.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB90608; AAB90608; AF_0633.
GeneIDi1483851.
KEGGiafu:AF0633.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB90608.1.
PIRiA69329.
RefSeqiNP_069467.1. NC_000917.1.
WP_010878137.1. NC_000917.1.

3D structure databases

ProteinModelPortaliO29622.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224325.AF0633.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB90608; AAB90608; AF_0633.
GeneIDi1483851.
KEGGiafu:AF0633.

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiVLGDWDN.

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-648-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus."
    Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G.
    , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C.
    Nature 390:364-370(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

Entry informationi

Entry nameiSYI_ARCFU
AccessioniPrimary (citable) accession number: O29622
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: May 27, 2015
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.