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O29622

- SYI_ARCFU

UniProt

O29622 - SYI_ARCFU

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei589 – 5891ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciAFUL224325:GJBC-648-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:AF_0633
    OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
    Taxonomic identifieri224325 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
    ProteomesiUP000002199: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10181018Isoleucine--tRNA ligasePRO_0000098575Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi224325.AF0633.

    Structurei

    3D structure databases

    ProteinModelPortaliO29622.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi43 – 5311"HIGH" regionAdd
    BLAST
    Motifi586 – 5905"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    KOiK01870.
    OMAiKPVHWCL.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O29622-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLPAKYSPKD VESEIFKFWE DNDIYRKVKE KGGRKFYFVD GPPYTTGRIH     50
    LGTAWNKVLK DTILRYKRMM GFAPTDTPGW DMHGLPIEVK VEQELGFRTK 100
    RDIESFGIDK FIERCMNYAL ANKDAMTEQF KSLAVWMDWE NPYMTIKAEY 150
    MNAAWFAIKR AHERGLLERK KMVVNWCHRC ETALADAEVE YWDEEDPSIY 200
    VKFPVKGEKD TYIVIWTTTP WTLPANMAVA VHPSLEYAKF RAVKDGKVEY 250
    LILAKELADS VLGKGDYDSW EVVETYLGED LEGLEYEHPL ADEVPLQKNW 300
    KHQVFFADFV TAENTGCVHI APGHGVEDYE LGVEKGLEVF NPVDDRGVYT 350
    EEAGKYAGKH VKEANDDIID DLYRKDLLLA EERIVHRYGH CWRCKTPIIY 400
    RATEQWFIKI SELKDEMLEE IDKVMWIPEW AGSARFKDWV SNAKDWCISR 450
    QRYWGIPIPV WICEKCGEMK VVGSINEIEW ENDLDLHRPK IDAVTFSCQC 500
    GGVMRRVPDV FDVWFDSGVA SWGSIAYPLR KDKFEELWPA DFITEGHDQT 550
    RGWFYSQLGT SVVCFDKAPY KAVLMHGFTL DEQGRKMSKS LGNVVEPEEV 600
    VGQIGVDGFR LYVLYSAPWE DLRFSWEEAR NINRMLNVVW NAVRFAHTYM 650
    SLDNYSFGEK GELKIEDRWI LSRLESFIKE ANEAMEGYQV HRVVRAFFDF 700
    FVEDFSRWYI QIIRPRVWEE RDSPSKLAAY YTMFRVIDRS LRAIAPFAPL 750
    IAEWFYQHVV KEFREGEESI FMEEYSTAEV EMIDGELEAA MKVAKEIVEA 800
    AANARNKAKR KLRWPLRELV IESGSEKVRK AVEMLEETIL SQCNVKQVRV 850
    VDSFEKEIEI KPNYKYIGPL LKEKAGEFAK YVASLKEIPE KLVFDGVELD 900
    PKQAIVVDYR LPEGYEYAEF SGGVVYIYKE LDDELVREAF AREVIRRIQE 950
    MRKELDLDVE EFIETTVEMD AELVKGWEDY IKSETRSQKL VFGKAEGYVR 1000
    EWNIEGKKVK IGIKRLRG 1018
    Length:1,018
    Mass (Da):118,885
    Last modified:January 1, 1998 - v1
    Checksum:i58C8BBE472FF1D0F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000782 Genomic DNA. Translation: AAB90608.1.
    PIRiA69329.
    RefSeqiNP_069467.1. NC_000917.1.

    Genome annotation databases

    EnsemblBacteriaiAAB90608; AAB90608; AF_0633.
    GeneIDi1483851.
    KEGGiafu:AF0633.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000782 Genomic DNA. Translation: AAB90608.1 .
    PIRi A69329.
    RefSeqi NP_069467.1. NC_000917.1.

    3D structure databases

    ProteinModelPortali O29622.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224325.AF0633.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB90608 ; AAB90608 ; AF_0633 .
    GeneIDi 1483851.
    KEGGi afu:AF0633.

    Phylogenomic databases

    eggNOGi COG0060.
    KOi K01870.
    OMAi KPVHWCL.

    Enzyme and pathway databases

    BioCyci AFUL224325:GJBC-648-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus."
      Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G.
      , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C.
      Nature 390:364-370(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

    Entry informationi

    Entry nameiSYI_ARCFU
    AccessioniPrimary (citable) accession number: O29622
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3