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O29610 (IDH_ARCFU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Isocitrate dehydrogenase [NADP]

Short name=IDH
EC=1.1.1.42
Alternative name(s):
IDP
NADP(+)-specific ICDH
Oxalosuccinate decarboxylase
Gene names
Name:icd
Ordered Locus Names:AF_0647
OrganismArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) [Reference proteome] [HAMAP]
Taxonomic identifier224325 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus

Protein attributes

Sequence length412 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Subunit structure

Homodimer.

Sequence similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Biophysicochemical properties

Kinetic parameters:

KM=118 µM for D,L-isocitrate

KM=30 µM for NADP

KM=5.4 mM for NAD

Vmax=141 µmol/min/mg enzyme towards NADP at 60 degrees Celsius

Vmax=14.5 µmol/min/mg enzyme towards NAD

pH dependence:

Optimum pH is 8.6.

Temperature dependence:

Optimum temperature is 90 degrees Celsius. Thermostable.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 412412Isocitrate dehydrogenase [NADP]
PRO_0000083572

Regions

Nucleotide binding333 – 3397NADP By similarity

Sites

Metal binding3011Magnesium or manganese By similarity
Binding site1001NADP By similarity
Binding site1091Substrate By similarity
Binding site1111Substrate By similarity
Binding site1151Substrate By similarity
Binding site1251Substrate By similarity
Binding site1491Substrate By similarity
Binding site3461NADP; via amide nitrogen and carbonyl oxygen By similarity
Binding site3851NADP By similarity
Binding site3891NADP By similarity
Site1561Critical for catalysis By similarity
Site2231Critical for catalysis By similarity

Amino acid modifications

Modified residue1091Phosphoserine By similarity

Secondary structure

........................................................................... 412
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O29610 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: A2E1A3AA7662D3EA

FASTA41245,842
        10         20         30         40         50         60 
MQYEKVKPPE NGEKIRYENG KLIVPDNPII PYFEGDGIGK DVVPAAIRVL DAAADKIGKE 

        70         80         90        100        110        120 
VVWFQVYAGE DAYKLYGNYL PDDTLNAIKE FRVALKGPLT TPVGGGYRSL NVTIRQVLDL 

       130        140        150        160        170        180 
YANVRPVYYL KGVPSPIKHP EKVNFVIFRE NTEDVYAGIE WPRGSEEALK LIRFLKNEFG 

       190        200        210        220        230        240 
VTIREDSGIG IKPISEFATK RLVRMAIRYA IENNRKSVTL VHKGNIMKYT EGAFRDWGYE 

       250        260        270        280        290        300 
VAKQEFGEYC ITEDELWDKY GGKQPEGKIV VKDRIADNMF QQILTRTDEY DVIALPNLNG 

       310        320        330        340        350        360 
DYLSDAAAAL IGGLGIAPGS NIGDGIGVFE PVHGSAPKYA GQNKVNPTAE ILTGALMFEY 

       370        380        390        400        410 
IGWKDASEMI KKAVEMTISS GIVTYDIHRH MGGTKVGTRE FAEAVVENLQ SL 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z96105 Genomic DNA. Translation: CAB09535.1.
AE000782 Genomic DNA. Translation: AAB90591.1.
PIRG69330.
RefSeqNP_069481.1. NC_000917.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2IV0X-ray2.50A/B1-412[»]
ProteinModelPortalO29610.
SMRO29610. Positions 1-412.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224325.AF0647.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB90591; AAB90591; AF_0647.
GeneID1483865.
KEGGafu:AF0647.

Phylogenomic databases

eggNOGCOG0538.
KOK00031.
OMACLRPIRY.

Enzyme and pathway databases

BioCycAFUL224325:GJBC-662-MONOMER.

Family and domain databases

Gene3D3.40.718.10. 1 hit.
InterProIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004439. Isocitrate_DH_NADP_dimer_prok.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERPTHR11835. PTHR11835. 1 hit.
PfamPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsTIGR00183. prok_nadp_idh. 1 hit.
PROSITEPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceO29610.

Entry information

Entry nameIDH_ARCFU
AccessionPrimary (citable) accession number: O29610
Secondary accession number(s): O31221
Entry history
Integrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: January 1, 1998
Last modified: May 14, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references