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Reviewed, UniProtKB/Swiss-Prot O29610 (IDH_ARCFU)

Last modified June 16, 2009. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Isocitrate dehydrogenase [NADP]
      Short name=IDH
    EC=1.1.1.42
Alternative name(s):
    Oxalosuccinate decarboxylase
    NADP(+)-specific ICDH
    IDP
Gene names
Name: icd
Ordered Locus Names: AF_0647
OrganismArchaeoglobus fulgidus [Complete proteome] [HAMAP]
Taxonomic identifier2234 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

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Protein attributes

Sequence length412 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Catalytic activity

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Oxalosuccinate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Subunit structure

Homodimer.

Sequence similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family.

biophysicochemical properties

Kinetic parameters:

KM=118 µM for D,L-isocitrate

KM=30 µM for NADP

KM=5.4 mM for NAD

Vmax=141 µmol/min/mg enzyme towards NADP at 60 degrees Celsius

Vmax=14.5 µmol/min/mg enzyme towards NAD

pH dependence:

Optimum pH is 8.6.

Temperature dependence:

Optimum temperature is 90 degrees Celsius. Thermostable.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 412412Isocitrate dehydrogenase [NADP]
PRO_0000083572

Regions

Nucleotide binding333 – 3397NADP By similarity

Sites

Metal binding3011Magnesium or manganese By similarity
Binding site1001NADP By similarity
Binding site1091Substrate By similarity
Binding site1111Substrate By similarity
Binding site1151Substrate By similarity
Binding site1251Substrate By similarity
Binding site1491Substrate By similarity
Binding site3461NADP; via amide nitrogen and carbonyl oxygen By similarity
Binding site3851NADP By similarity
Binding site3891NADP By similarity
Site1561Critical for catalysis By similarity
Site2231Critical for catalysis By similarity

Amino acid modifications

Modified residue1091Phosphoserine By similarity

Secondary structure

1 .......................................................................... 412
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
O29610-1 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: A2E1A3AA7662D3EA

FASTA41245,842
        10         20         30         40         50         60 
MQYEKVKPPE NGEKIRYENG KLIVPDNPII PYFEGDGIGK DVVPAAIRVL DAAADKIGKE 

        70         80         90        100        110        120 
VVWFQVYAGE DAYKLYGNYL PDDTLNAIKE FRVALKGPLT TPVGGGYRSL NVTIRQVLDL 

       130        140        150        160        170        180 
YANVRPVYYL KGVPSPIKHP EKVNFVIFRE NTEDVYAGIE WPRGSEEALK LIRFLKNEFG 

       190        200        210        220        230        240 
VTIREDSGIG IKPISEFATK RLVRMAIRYA IENNRKSVTL VHKGNIMKYT EGAFRDWGYE 

       250        260        270        280        290        300 
VAKQEFGEYC ITEDELWDKY GGKQPEGKIV VKDRIADNMF QQILTRTDEY DVIALPNLNG 

       310        320        330        340        350        360 
DYLSDAAAAL IGGLGIAPGS NIGDGIGVFE PVHGSAPKYA GQNKVNPTAE ILTGALMFEY 

       370        380        390        400        410 
IGWKDASEMI KKAVEMTISS GIVTYDIHRH MGGTKVGTRE FAEAVVENLQ SL

« Hide

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Cross-references

Sequence databases

Z96105 Genomic DNA. Translation: CAB09535.1.
AE000782 Genomic DNA. Translation: AAB90591.1.
PIRG69330.
RefSeqNP_069481.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2IV0X-ray2.50A/B1-412[»]
ModBaseSearch...

Genome annotation databases

GeneID1483865.
GenomeReviewsGene locus AF_0647 in contig AE000782_GR.
KEGGafu:AF0647.
NMPDRfig|224325.1.peg.640.
TIGRAF_0647.

Phylogenomic databases

HOGENOMO29610.
OMAO29610. MGWNEAA.

Enzyme and pathway databases

BioCycAFUL224325:AF_0647-MON.
BRENDA1.1.1.42. 7576.

Family and domain databases

InterProIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004439. Isocitrate_DH_NADP_prok.
[Graphical view]
Gene3DG3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHERPTHR11835. IDH_IMDH_dimeric. 1 hit.
PTHR11835:SF1. NADP_IDH_prok. 1 hit.
PfamPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsTIGR00183. prok_nadp_idh. 1 hit.
PROSITEPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameIDH_ARCFU
AccessionPrimary (citable) accession number: O29610
Secondary accession number(s): O31221
Entry history
Integrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: January 1, 1998
Last modified: June 16, 2009
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents