O29592 (BUD32_ARCFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable serine/threonine-protein kinase BUD32 homolog
EC=2.7.11.1

Gene names

Ordered Locus Names:

AF_0665

Organism

Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) [Reference proteome] [HAMAP]

Taxonomic identifier

224325 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus › Archaeoglobus fulgidus

Protein attributes

Sequence length	195 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Could be involved in the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 195	195	Probable serine/threonine-protein kinase BUD32 homolog		PRO_0000096983
Regions
	Domain	1 – 195	195	Protein kinase
Sites
	Active site	107	1	Proton acceptor By similarity
	Binding site	12	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	O29592 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 949594C57A000A24	FASTA	195	22,380
	10 20 30 40 50 60 MKVYLGGEAE VKILENVVVK TRIPKRYRIK ELDRELRLRR TKMEAKIISA ARRAGVPTPI 70 80 90 100 110 120 VLDVEEDTIV MERIYGEAVK DVMSKDVSRE VGRMAAKLHR AGIIHGDITP MNLILSNSRI 130 140 150 160 170 180 YFVDFGLAFF DNKVEPMGVD VHVYFESLKA SFENWERLRD AFIEGYLAEG GSEEVIERAK 190 EIEERGRYVE RVSMG

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000782 Genomic DNA. Translation: AAB90576.1.
PIR	A69333.
RefSeq	NP_069499.1. NC_000917.1.
3D structure databases
ProteinModelPortal	O29592.
ModBase	Search...
Protein-protein interaction databases
STRING	224325.AF0665.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB90576; AAB90576; AF_0665.
GeneID	1483883.
KEGG	afu:AF0665.
Phylogenomic databases
eggNOG	COG3642.
KO	K07174.
OMA	AEVRIYE.
ProtClustDB	PRK14879.
Enzyme and pathway databases
BioCyc	AFUL224325:GJBC-680-MONOMER.
Family and domain databases
InterPro	IPR022495. Kae1-assoc_kinase_Bud32. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR018934. RIO-like_kinase. IPR008266. Tyr_kinase_AS. [Graphical view]
Pfam	PF01163. RIO1. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
TIGRFAMs	TIGR03724. arch_bud32. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BUD32_ARCFU

Accession

Primary (citable) accession number: O29592

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program