Reviewed, UniProtKB/Swiss-Prot O29548 (PPSA_ARCFU)

Names and origin

Protein names

Recommended name:
    Probable phosphoenolpyruvate synthase
      Short name=PEP synthase
    EC=2.7.9.2
Alternative name(s):
    Pyruvate, water dikinase

Gene names

Name:	ppsA
Ordered Locus Names:	AF_0710

Organism

Archaeoglobus fulgidus [Complete proteome] [HAMAP]

Taxonomic identifier

2234 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	753 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate By similarity.
Catalytic activity	ATP + pyruvate + H2O = AMP + phosphoenolpyruvate + phosphate.
Cofactor	Magnesium By similarity.
Pathway	Carbohydrate biosynthesis; gluconeogenesis.
Domain	The N-terminal domain contains the ATP/Pi binding site, the central domain the pyrophosphate/phosphate carrier histidine, and the C-terminal domain the pyruvate binding site By similarity.
Miscellaneous	The reaction takes place in three steps, mediated by a phosphocarrier histidine residue located on the surface of the central domain. The two first partial reactions are catalyzed at an active site located on the N-terminal domain, and the third partial reaction is catalyzed at an active site located on the C-terminal domain. For catalytic turnover, the central domain swivels from the concave surface of the N-terminal domain to that of the C-terminal domain By similarity.
Sequence similarities	Belongs to the PEP-utilizing enzyme family.

Ontologies

Keywords
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: InterPro phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW pyruvate, water dikinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 753	753	Probable phosphoenolpyruvate synthase		PRO_0000147041
Sites
	Active site	398	1	Tele-phosphohistidine intermediate By similarity
	Active site	703	1	Proton donor By similarity
	Metal binding	631	1	Magnesium By similarity
	Metal binding	656	1	Magnesium By similarity
	Binding site	488	1	Substrate By similarity
	Binding site	535	1	Substrate By similarity
	Binding site	631	1	Substrate By similarity
	Binding site	653	1	Substrate; via carbonyl oxygen By similarity
	Binding site	654	1	Substrate; via amide nitrogen By similarity
	Binding site	655	1	Substrate By similarity
	Binding site	656	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	O29548-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 3D087F950321A83B	FASTA	753	83,868
	10 20 30 40 50 60 MPVLWLADVD KNDIPLVGGK GANLGELLRA EIPVPDGFVV DARTFREFIQ KTGIAEKIYS 70 80 90 100 110 120 LLRELDVEDT EKLDAVSREI REIIEKTEMP EDIEREIREA YRKLCEEEGK EVYVAVRSSA 130 140 150 160 170 180 TAEDLPDASF AGQQETYLNV VGEDEVVEKV KKCWGSLFTP RAIYYRVQKG FRHEDVSIAV 190 200 210 220 230 240 VVQKMVNSEK SGVMFTSHPV SGEKKCIIEA VFGLGEAIVS GLVTPDTYVY DRVKRKIEEV 250 260 270 280 290 300 KIGEKKFMLT RKDGKTVKVE LPPEKANERV LSDEEIEKLV TLGELIEDHY GKPQDVEWAI 310 320 330 340 350 360 EGGKIYIVQS RPITTIRKEK KEAEEEVSEE AEGKILLKGL GASPGIASGK VKVIFSEKEI 370 380 390 400 410 420 SKVEEGDILV TTMTTPDMVP AMKRAAAIVT DEGGMTCHAA IVSRELGVPA VVGTKVATKV 430 440 450 460 470 480 LKDGMVVTVD GEKGIVYEGR IEKKEEPKPV VASAPIITAT EVKVNISIPD VAERVARETN 490 500 510 520 530 540 ADGVGLFRIE HMVLGLEKHP MKFIRDGEID RYIDLLYQEM KKVVKAFYPK PVWIRTIDAP 550 560 570 580 590 600 TDEFRAMEGG EDEPIEANPM LGFRGIRRDL AEEEHFRAEM RAIKKLVDEG YTNVGVMLPL 610 620 630 640 650 660 ITSPEEVKRA KEIAISEGLP LDKIEFGVMV ETPAAALILE DIIKEGIDFV SLGTNDLTQY 670 680 690 700 710 720 TLAVDRNNEN VAYLYNETHP AVLKLIERTI KVCKEHGVKS SICGQAGSYP HVVEKLVEFG 730 740 750 IDSVSANPDA VQRIREVVAR AEKRIILEKL RKI

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000782 Genomic DNA. Translation: AAB90532.1.
PIR	F69338.
RefSeq	NP_069544.1.
3D structure databases
HSSP	HSSP built from PDB template 1H6Z based on UniProtKB O76283.
ModBase	Search...
Genome annotation databases
GeneID	1483928.
GenomeReviews	Gene locus AF_0710 in contig AE000782_GR.
KEGG	afu:AF0710.
NMPDR	fig|224325.1.peg.703.
TIGR	AF_0710.
Phylogenomic databases
HOGENOM	O29548.
OMA	RAEHMIL.
Enzyme and pathway databases
BioCyc	AFUL224325:AF_0710-MON.
BRENDA	2.7.9.2. 7576.
Family and domain databases
InterPro	IPR013815. ATP_grasp_subdomain_1. IPR008279. PEP_mobile. IPR018274. PEP_mobile_CS. IPR006318. PEP_P_trans. IPR006319. PEP_synth. IPR000121. PEP_utilizers. IPR002192. PPDK_PEP_bd. IPR015813. Pyrv/PenolPyrv_Kinase_cat. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.50.30.10. PEP_mobile. 1 hit. G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
Pfam	PF00391. PEP-utilizers. 1 hit. PF02896. PEP-utilizers_C. 1 hit. PF01326. PPDK_N. 1 hit. [Graphical view]
PRINTS	PR01736. PHPHTRNFRASE.
ProDom	PD000940. PEP_utilizers. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01418. PEP_synth. 1 hit.
PROSITE	PS00742. PEP_ENZYMES_2. 1 hit. PS00370. PEP_ENZYMES_PHOS_SITE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPSA_ARCFU

Accession

Primary (citable) accession number: O29548

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	November 3, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)