ID   MTD_ARCFU               Reviewed;         273 AA.
AC   O29544; Q9UWM0;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   16-JUN-2009, entry version 63.
DE   RecName: Full=F420-dependent methylenetetrahydromethanopterin dehydrogenase;
DE            Short=MTD;
DE            EC=1.5.99.9;
DE   AltName: Full=Coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin dehydrogenase;
GN   Name=mtd; OrderedLocusNames=AF_0714;
OS   Archaeoglobus fulgidus.
OC   Archaea; Euryarchaeota; Archaeoglobi; Archaeoglobales;
OC   Archaeoglobaceae; Archaeoglobus.
OX   NCBI_TaxID=2234;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126;
RX   MEDLINE=98049343; PubMed=9389475; DOI=10.1038/37052;
RA   Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E.,
RA   Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D.,
RA   Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C.,
RA   Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G., Gill S.R.,
RA   Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J.,
RA   Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A.,
RA   Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A.,
RA   Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P.,
RA   Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C.,
RA   Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O.,
RA   Woese C.R., Venter J.C.;
RT   "The complete genome sequence of the hyperthermophilic, sulphate-
RT   reducing archaeon Archaeoglobus fulgidus.";
RL   Nature 390:364-370(1997).
RN   [2]
RP   PROTEIN SEQUENCE OF 2-34, AND CHARACTERIZATION.
RC   STRAIN=ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126;
RX   MEDLINE=93243884; PubMed=8481089; DOI=10.1007/BF00248476;
RA   Schworer B., Breitung J., Klein A.R., Stetter K.O., Thauer R.K.;
RT   "Formylmethanofuran: tetrahydromethanopterin formyltransferase and
RT   N5,N10-methylenetetrahydromethanopterin dehydrogenase from the
RT   sulfate-reducing Archaeoglobus fulgidus: similarities with the enzymes
RT   from methanogenic Archaea.";
RL   Arch. Microbiol. 159:225-232(1993).
CC   -!- FUNCTION: Catalyzes the oxidation of methylene-H(4)MPT to
CC       methenyl-H(4)MPT(+).
CC   -!- CATALYTIC ACTIVITY: 5,10-methylenetetrahydromethanopterin +
CC       coenzyme F420 = 5,10-methenyltetrahydromethanopterin + reduced
CC       coenzyme F420.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is about 5.5;
CC       Temperature dependence:
CC         Optimum temperature is 70 degrees Celsius. Thermostable up to 90
CC         degrees Celsius, however, only in the presence of salts;
CC   -!- PATHWAY: Metabolic intermediate metabolism; lactic acid oxidation.
CC   -!- SIMILARITY: Belongs to the MTD family.
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DR   EMBL; AE000782; AAB90526.1; -; Genomic_DNA.
DR   PIR; B69339; B69339.
DR   RefSeq; NP_069548.1; -.
DR   GeneID; 1483932; -.
DR   GenomeReviews; AE000782_GR; AF_0714.
DR   KEGG; afu:AF0714; -.
DR   NMPDR; fig|224325.1.peg.707; -.
DR   TIGR; AF_0714; -.
DR   HOGENOM; O29544; -.
DR   OMA; O29544; NPYAKAK.
DR   BioCyc; AFUL224325:AF_0714-MON; -.
DR   BRENDA; 1.5.99.9; 7576.
DR   GO; GO:0008901; F:ferredoxin hydrogenase activity; IEA:InterPro.
DR   GO; GO:0030268; F:methylenetetrahydromethanopterin dehydrogen...; IEA:HAMAP.
DR   GO; GO:0015948; P:methanogenesis; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_00058; -; 1.
DR   InterPro; IPR002844; Methylene_DH.
DR   Pfam; PF01993; MTD; 1.
DR   PIRSF; PIRSF005627; MTD; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; Direct protein sequencing; One-carbon metabolism;
KW   Oxidoreductase.
FT   INIT_MET      1      1       Removed.
FT   CHAIN         2    273       F420-dependent
FT                                methylenetetrahydromethanopterin
FT                                dehydrogenase.
FT                                /FTId=PRO_0000075036.
SQ   SEQUENCE   273 AA;  29645 MW;  335B2432D5FE7B7E CRC64;
     MVVKVGVLKM GAIGTALLVE YLLDERADRE DIEVRVVTSG AKMQPEEAVV AEKLKEFDPD
     VVIVVSPNAA LPGPKAAREA FEGKPVIVIS DAPAKKAKDE LKEKGFGYIL INADSMIGAR
     REFLDPTEMA LFNADVVKVL AATGAFRLVQ EAIDKVIEDI KAGKQPELPQ IVVTAEKAVE
     AGKFSNPYAK AKAMAAFYIA EKVADIDVKG CFIEKDPEKY IPLVASAHEM MRIAAILADQ
     AREIEKSNDT VFRNPHAKDG KILGKTQLMA KPE
//