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Reviewed, UniProtKB/Swiss-Prot O29544 (MTD_ARCFU)

Last modified March 2, 2010. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
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Names and originHide

Protein namesRecommended name:
F420-dependent methylenetetrahydromethanopterin dehydrogenase

Short name=MTD
EC=1.5.99.9
Alternative name(s):
Coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin dehydrogenase
Gene names
Name:mtd
Ordered Locus Names:AF_0714
OrganismArchaeoglobus fulgidus [Complete proteome] [HAMAP]
Taxonomic identifier2234 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
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Protein attributesHide

Sequence length273 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.
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General annotation (Comments)Hide

Function

Catalyzes the oxidation of methylene-H4MPT to methenyl-H4MPT+. HAMAP MF_00058

Catalytic activity

5,10-methylenetetrahydromethanopterin + coenzyme F420 = 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420. HAMAP MF_00058

Pathway

Metabolic intermediate metabolism; lactate oxidation. HAMAP MF_00058

Sequence similarities

Belongs to the MTD family.

Biophysicochemical properties

pH dependence:

Optimum pH is about 5.5. HAMAP MF_00058

Temperature dependence:

Optimum temperature is 70 degrees Celsius. Thermostable up to 90 degrees Celsius, however, only in the presence of salts.

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Sequence annotation (Features)Hide

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.2
Chain2 – 273272F420-dependent methylenetetrahydromethanopterin dehydrogenase HAMAP MF_00058
PRO_0000075036
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SequencesHide

Sequence LengthMass (Da)Tools
O29544-1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 335B2432D5FE7B7E

FASTA27329,645
        10         20         30         40         50         60 
MVVKVGVLKM GAIGTALLVE YLLDERADRE DIEVRVVTSG AKMQPEEAVV AEKLKEFDPD 

        70         80         90        100        110        120 
VVIVVSPNAA LPGPKAAREA FEGKPVIVIS DAPAKKAKDE LKEKGFGYIL INADSMIGAR 

       130        140        150        160        170        180 
REFLDPTEMA LFNADVVKVL AATGAFRLVQ EAIDKVIEDI KAGKQPELPQ IVVTAEKAVE 

       190        200        210        220        230        240 
AGKFSNPYAK AKAMAAFYIA EKVADIDVKG CFIEKDPEKY IPLVASAHEM MRIAAILADQ 

       250        260        270 
AREIEKSNDT VFRNPHAKDG KILGKTQLMA KPE 

« Hide

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ReferencesHide

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Cross-referencesHide

Sequence databases

EMBL
GenBank
DDBJ
AE000782 Genomic DNA. Translation: AAB90526.1.
PIRB69339.
RefSeqNP_069548.1.

3D structure databases

SMRO29544. Positions 1-273.
ModBaseSearch...

Genome annotation databases

GeneID1483932.
GenomeReviewsGene locus AF_0714 in contig AE000782_GR.
KEGGafu:AF0714.
NMPDRfig|224325.1.peg.707.
TIGRAF_0714.

Phylogenomic databases

HOGENOMHBG538567.
OMAIVASAHE.
ProtClustDBPRK00994.

Enzyme and pathway databases

BioCycAFUL224325:AF_0714-MONOMER.
BRENDA1.5.99.9. 7576.

Family and domain databases

HAMAPMF_00058. MTD.
[Tree]
InterProIPR002844. Methylene_DH.
[Graphical view]
PfamPF01993. MTD. 1 hit.
[Graphical view]
PIRSFPIRSF005627. MTD. 1 hit.
ProDomPD017604. Methylene_DH. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF102324. MeTD. 1 hit.
ProtoNetSearch...
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Entry informationHide

Entry nameMTD_ARCFU
AccessionPrimary (citable) accession number: O29544
Secondary accession number(s): Q9UWM0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: March 2, 2010
This is version 69 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)
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Relevant documentsHide

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents