O29544 (MTD_ARCFU) Reviewed, UniProtKB/Swiss-Prot
Last modified
November 16, 2011.
Version 77.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: F420-dependent methylenetetrahydromethanopterin dehydrogenase Short name=MTD EC=1.5.99.9 Alternative name(s): Coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin dehydrogenase | ||||
| Gene names |
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| Organism | Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) | ||||
| Taxonomic identifier | 224325 [NCBI] | ||||
| Taxonomic lineage | Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus |
Protein attributes
| Sequence length | 273 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Catalyzes the oxidation of methylene-H4MPT to methenyl-H4MPT+. HAMAP MF_00058 |
| Catalytic activity | 5,10-methylenetetrahydromethanopterin + coenzyme F420 = 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420. HAMAP MF_00058 |
| Pathway | Metabolic intermediate metabolism; lactate oxidation. HAMAP MF_00058 |
| Sequence similarities | Belongs to the MTD family. |
| Biophysicochemical properties | pH dependence: Optimum pH is about 5.5. HAMAP MF_00058 Temperature dependence: Optimum temperature is 70 degrees Celsius. Thermostable up to 90 degrees Celsius, however, only in the presence of salts. |
Ontologies
| Keywords | |
|---|---|
| Biological process | One-carbon metabolism |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome Direct protein sequencing Reference proteome |
| Gene Ontology (GO) | |
| Biological process | methanogenesis Inferred from electronic annotation. Source: InterPro one-carbon metabolic processInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro methylenetetrahydromethanopterin dehydrogenase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | |||
Molecule processing | ||||||||
|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed Ref.2 | |||||
| Chain | 2 – 273 | 272 | F420-dependent methylenetetrahydromethanopterin dehydrogenase HAMAP MF_00058 | PRO_0000075036 | ||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. Venter J.C.Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126. |
| [2] | "Formylmethanofuran: tetrahydromethanopterin formyltransferase and N5,N10-methylenetetrahydromethanopterin dehydrogenase from the sulfate-reducing Archaeoglobus fulgidus: similarities with the enzymes from methanogenic Archaea." Schworer B., Breitung J., Klein A.R., Stetter K.O., Thauer R.K. Arch. Microbiol. 159:225-232(1993) [PubMed: 8481089] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-34, CHARACTERIZATION. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AE000782 Genomic DNA. Translation: AAB90526.1. |
| PIR | B69339. |
| RefSeq | NP_069548.1. NC_000917.1. |
3D structure databases | |
| ProteinModelPortal | O29544. |
| SMR | O29544. Positions 1-273. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 1483932. |
| GenomeReviews | Gene locus AF_0714 in contig AE000782_GR. |
| KEGG | afu:AF0714. |
| NMPDR | fig|224325.1.peg.707. |
| TIGR | AF_0714. |
Phylogenomic databases | |
| HOGENOM | HBG538567. |
| OMA | NPYAKAK. |
| ProtClustDB | PRK00994. |
Enzyme and pathway databases | |
| BioCyc | AFUL224325:AF_0714-MONOMER. |
Family and domain databases | |
| HAMAP | MF_00058. MTD. [Tree] |
| InterPro | IPR002844. Methylene_DH. [Graphical view] |
| KO | K00319. |
| Pfam | PF01993. MTD. 1 hit. [Graphical view] |
| PIRSF | PIRSF005627. MTD. 1 hit. |
| ProDom | PD017604. Methylene_DH. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| SUPFAM | SSF102324. MeTD. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | MTD_ARCFU | ||||||||
| Accession | Primary (citable) accession number: O29544 Secondary accession number(s): Q9UWM0 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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