O29544 (MTD_ARCFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
F420-dependent methylenetetrahydromethanopterin dehydrogenase
Short name=MTD
EC=1.5.99.9

Alternative name(s):
Coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin dehydrogenase

Gene names

Name:	mtd
Ordered Locus Names:	AF_0714

Organism

Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)

Taxonomic identifier

224325 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the oxidation of methylene-H4MPT to methenyl-H4MPT+. HAMAP MF_00058
Catalytic activity	5,10-methylenetetrahydromethanopterin + coenzyme F420 = 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420. HAMAP MF_00058
Pathway	Metabolic intermediate metabolism; lactate oxidation. HAMAP MF_00058
Sequence similarities	Belongs to the MTD family.
Biophysicochemical properties	pH dependence: Optimum pH is about 5.5. HAMAP MF_00058 Temperature dependence: Optimum temperature is 70 degrees Celsius. Thermostable up to 90 degrees Celsius, however, only in the presence of salts.

Ontologies

Keywords
Biological process	One-carbon metabolism
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro methylenetetrahydromethanopterin dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 273	272	F420-dependent methylenetetrahydromethanopterin dehydrogenase HAMAP MF_00058		PRO_0000075036

Sequences

	Sequence		Length	Mass (Da)
	O29544 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 335B2432D5FE7B7E	FASTA	273	29,645
	10 20 30 40 50 60 MVVKVGVLKM GAIGTALLVE YLLDERADRE DIEVRVVTSG AKMQPEEAVV AEKLKEFDPD 70 80 90 100 110 120 VVIVVSPNAA LPGPKAAREA FEGKPVIVIS DAPAKKAKDE LKEKGFGYIL INADSMIGAR 130 140 150 160 170 180 REFLDPTEMA LFNADVVKVL AATGAFRLVQ EAIDKVIEDI KAGKQPELPQ IVVTAEKAVE 190 200 210 220 230 240 AGKFSNPYAK AKAMAAFYIA EKVADIDVKG CFIEKDPEKY IPLVASAHEM MRIAAILADQ 250 260 270 AREIEKSNDT VFRNPHAKDG KILGKTQLMA KPE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.
[2]	"Formylmethanofuran: tetrahydromethanopterin formyltransferase and N5,N10-methylenetetrahydromethanopterin dehydrogenase from the sulfate-reducing Archaeoglobus fulgidus: similarities with the enzymes from methanogenic Archaea." Schworer B., Breitung J., Klein A.R., Stetter K.O., Thauer R.K. Arch. Microbiol. 159:225-232(1993) [PubMed: 8481089] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-34, CHARACTERIZATION. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000782 Genomic DNA. Translation: AAB90526.1.
PIR	B69339.
RefSeq	NP_069548.1. NC_000917.1.
3D structure databases
ProteinModelPortal	O29544.
SMR	O29544. Positions 1-273.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1483932.
GenomeReviews	Gene locus AF_0714 in contig AE000782_GR.
KEGG	afu:AF0714.
NMPDR	fig|224325.1.peg.707.
TIGR	AF_0714.
Phylogenomic databases
HOGENOM	HBG538567.
OMA	NPYAKAK.
ProtClustDB	PRK00994.
Enzyme and pathway databases
BioCyc	AFUL224325:AF_0714-MONOMER.
Family and domain databases
HAMAP	MF_00058. MTD. [Tree]
InterPro	IPR002844. Methylene_DH. [Graphical view]
KO	K00319.
Pfam	PF01993. MTD. 1 hit. [Graphical view]
PIRSF	PIRSF005627. MTD. 1 hit.
ProDom	PD017604. Methylene_DH. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF102324. MeTD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTD_ARCFU

Accession

Primary (citable) accession number: O29544
Secondary accession number(s): Q9UWM0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 23, 2007
Last modified:	November 16, 2011
This is version 77 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program