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Protein

Sirohydrochlorin cobaltochelatase

Gene

cbiX

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the insertion of Co2+ into sirohydrochlorin as part of the anaerobic pathway to cobalamin biosynthesis.1 Publication

Catalytic activityi

Cobalt-sirohydrochlorin + 2 H+ = sirohydrochlorin + Co2+.1 Publication

Pathwayi: adenosylcobalamin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route).
Proteins known to be involved in the 10 steps of the subpathway in this organism are:
  1. Sirohydrochlorin cobaltochelatase (cbiX)
  2. no protein annotated in this organism
  3. Cobalamin biosynthesis protein CbiHC (cbiHC)
  4. no protein annotated in this organism
  5. no protein annotated in this organism
  6. Cobalt-precorrin-5B C(1)-methyltransferase (cbiD)
  7. no protein annotated in this organism
  8. Probable cobalt-precorrin-6B C(15)-methyltransferase (decarboxylating) (cbiT)
  9. Cobalamin biosynthesis protein CbiHC (cbiHC)
  10. Cobyrinate a,c-diamide synthase (cbiA)
This subpathway is part of the pathway adenosylcobalamin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route), the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei10Proton acceptorSequence analysis1
Metal bindingi10Cobalt1
Binding sitei46Substrate1
Metal bindingi74CobaltCurated1

GO - Molecular functioni

  • cobalt ion binding Source: UniProtKB
  • sirohydrochlorin cobaltochelatase activity Source: UniProtKB
  • tetrapyrrole binding Source: UniProtKB

GO - Biological processi

  • anaerobic cobalamin biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Cobalamin biosynthesis

Keywords - Ligandi

Cobalt, Metal-binding

Enzyme and pathway databases

BRENDAi4.99.1.3. 414.
UniPathwayiUPA00148; UER00223.

Names & Taxonomyi

Protein namesi
Recommended name:
Sirohydrochlorin cobaltochelatase (EC:4.99.1.3)
Alternative name(s):
CbiXS
Gene namesi
Name:cbiX
Ordered Locus Names:AF_0721
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001503531 – 132Sirohydrochlorin cobaltochelataseAdd BLAST132

Interactioni

Subunit structurei

Homodimer or homotetramer.1 Publication

Protein-protein interaction databases

DIPiDIP-59586N.
STRINGi224325.AF0721.

Structurei

Secondary structure

1132
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 9Combined sources7
Helixi15 – 31Combined sources17
Beta strandi33 – 43Combined sources11
Helixi50 – 56Combined sources7
Beta strandi60 – 66Combined sources7
Beta strandi68 – 71Combined sources4
Helixi73 – 76Combined sources4
Helixi78 – 83Combined sources6
Beta strandi88 – 90Combined sources3
Beta strandi92 – 96Combined sources5
Beta strandi99 – 103Combined sources5
Helixi111 – 122Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TJNX-ray2.01A1-132[»]
2DJ5X-ray2.55A/B1-132[»]
2XWQX-ray2.01A/B/C/D1-132[»]
2XWSX-ray1.60A1-132[»]
ProteinModelPortaliO29537.
SMRiO29537.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO29537.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni69 – 74Substrate binding6

Sequence similaritiesi

Belongs to the CbiX family. CbiXS subfamily.Curated

Phylogenomic databases

eggNOGiarCOG02246. Archaea.
COG2138. LUCA.
KOiK03795.
OMAiVICDPIG.

Family and domain databases

HAMAPiMF_00785. CbiX. 1 hit.
InterProiIPR002762. Cbl_biosynth_CbiX.
IPR023652. SiroHydchlorin_Cochelatase.
[Graphical view]
PfamiPF01903. CbiX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O29537-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRGLVIVGH GSQLNHYREV MELHRKRIEE SGAFDEVKIA FAARKRRPMP
60 70 80 90 100
DEAIREMNCD IIYVVPLFIS YGLHVTEDLP DLLGFPRGRG IKEGEFEGKK
110 120 130
VVICEPIGED YFVTYAILNS VFRIGRDGKG EE
Length:132
Mass (Da):15,109
Last modified:January 1, 1998 - v1
Checksum:i61B97A773EC78BB7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB90530.1.
PIRiA69340.
RefSeqiWP_010878224.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB90530; AAB90530; AF_0721.
GeneIDi24794319.
KEGGiafu:AF_0721.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB90530.1.
PIRiA69340.
RefSeqiWP_010878224.1. NC_000917.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TJNX-ray2.01A1-132[»]
2DJ5X-ray2.55A/B1-132[»]
2XWQX-ray2.01A/B/C/D1-132[»]
2XWSX-ray1.60A1-132[»]
ProteinModelPortaliO29537.
SMRiO29537.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59586N.
STRINGi224325.AF0721.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB90530; AAB90530; AF_0721.
GeneIDi24794319.
KEGGiafu:AF_0721.

Phylogenomic databases

eggNOGiarCOG02246. Archaea.
COG2138. LUCA.
KOiK03795.
OMAiVICDPIG.

Enzyme and pathway databases

UniPathwayiUPA00148; UER00223.
BRENDAi4.99.1.3. 414.

Miscellaneous databases

EvolutionaryTraceiO29537.

Family and domain databases

HAMAPiMF_00785. CbiX. 1 hit.
InterProiIPR002762. Cbl_biosynth_CbiX.
IPR023652. SiroHydchlorin_Cochelatase.
[Graphical view]
PfamiPF01903. CbiX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCBIX_ARCFU
AccessioniPrimary (citable) accession number: O29537
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.