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Protein

tRNA (cytidine(56)-2'-O)-methyltransferase

Gene

AF_0751

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Specifically catalyzes the AdoMet-dependent 2'-O-ribose methylation of cytidine at position 56 in tRNAs.By similarity

Catalytic activityi

S-adenosyl-L-methionine + cytidine(56) in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine(56) in tRNA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei79S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (cytidine(56)-2'-O)-methyltransferase (EC:2.1.1.206)
Alternative name(s):
tRNA ribose 2'-O-methyltransferase aTrm56
Gene namesi
Ordered Locus Names:AF_0751
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001469261 – 168tRNA (cytidine(56)-2'-O)-methyltransferaseAdd BLAST168

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi224325.AF0751.

Structurei

Secondary structure

1168
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 7Combined sources6
Helixi16 – 28Combined sources13
Beta strandi32 – 38Combined sources7
Helixi41 – 54Combined sources14
Beta strandi59 – 62Combined sources4
Helixi65 – 71Combined sources7
Beta strandi74 – 79Combined sources6
Beta strandi83 – 85Combined sources3
Helixi86 – 94Combined sources9
Beta strandi97 – 103Combined sources7
Helixi112 – 115Combined sources4
Beta strandi116 – 125Combined sources10
Helixi129 – 140Combined sources12
Turni144 – 146Combined sources3
Beta strandi153 – 157Combined sources5
Beta strandi160 – 162Combined sources3
Beta strandi164 – 166Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2O3AX-ray2.20A/B2-168[»]
ProteinModelPortaliO29507.
SMRiO29507.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO29507.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni104 – 108S-adenosyl-L-methionine bindingBy similarity5

Sequence similaritiesi

Belongs to the aTrm56 family.Curated

Phylogenomic databases

eggNOGiarCOG01857. Archaea.
COG1303. LUCA.
KOiK07254.
OMAiLGHRPER.

Family and domain databases

Gene3Di3.40.1280.10. 1 hit.
HAMAPiMF_00077. tRNA_methyltr_aTrm56. 1 hit.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR029026. tRNA_m1G_MTases_N.
IPR002845. tRNA_mtfrase_aTrm56.
[Graphical view]
PfamiPF01994. Trm56. 1 hit.
[Graphical view]
PIRSFiPIRSF016123. UCP016123. 1 hit.
ProDomiPD016584. DUF127. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF75217. SSF75217. 1 hit.

Sequencei

Sequence statusi: Complete.

O29507-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVYVLRLGH RPERDKRIST HVALTARAFG AKGIYFDTED KSVFESVRDV
60 70 80 90 100
VERWGGDFFI KAVSWKKLLR EFDGLKVHLT MYGIPLPQKL EEIKRADKVL
110 120 130 140 150
VVVGAEKVPP EVYELCDLNI SIGTQPHSEV AALAVFLDRV LGKVFDISFD
160
DAKIKVIPSE RGKRVVSE
Length:168
Mass (Da):19,026
Last modified:January 1, 1998 - v1
Checksum:iACEDE86A81D3C308
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB90489.1.
PIRiG69343.
RefSeqiWP_010878254.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB90489; AAB90489; AF_0751.
GeneIDi24794349.
KEGGiafu:AF_0751.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB90489.1.
PIRiG69343.
RefSeqiWP_010878254.1. NC_000917.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2O3AX-ray2.20A/B2-168[»]
ProteinModelPortaliO29507.
SMRiO29507.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224325.AF0751.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB90489; AAB90489; AF_0751.
GeneIDi24794349.
KEGGiafu:AF_0751.

Phylogenomic databases

eggNOGiarCOG01857. Archaea.
COG1303. LUCA.
KOiK07254.
OMAiLGHRPER.

Miscellaneous databases

EvolutionaryTraceiO29507.

Family and domain databases

Gene3Di3.40.1280.10. 1 hit.
HAMAPiMF_00077. tRNA_methyltr_aTrm56. 1 hit.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR029026. tRNA_m1G_MTases_N.
IPR002845. tRNA_mtfrase_aTrm56.
[Graphical view]
PfamiPF01994. Trm56. 1 hit.
[Graphical view]
PIRSFiPIRSF016123. UCP016123. 1 hit.
ProDomiPD016584. DUF127. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF75217. SSF75217. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRM56_ARCFU
AccessioniPrimary (citable) accession number: O29507
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.