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Protein

tRNA (cytidine(56)-2'-O)-methyltransferase

Gene

AF_0751

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Specifically catalyzes the AdoMet-dependent 2'-O-ribose methylation of cytidine at position 56 in tRNAs.By similarity

Catalytic activityi

S-adenosyl-L-methionine + cytidine(56) in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine(56) in tRNA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei79 – 791S-adenosyl-L-methionine; via carbonyl oxygenBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-767-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (cytidine(56)-2'-O)-methyltransferase (EC:2.1.1.206)
Alternative name(s):
tRNA ribose 2'-O-methyltransferase aTrm56
Gene namesi
Ordered Locus Names:AF_0751
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 168168tRNA (cytidine(56)-2'-O)-methyltransferasePRO_0000146926Add
BLAST

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi224325.AF0751.

Structurei

Secondary structure

1
168
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 76Combined sources
Helixi16 – 2813Combined sources
Beta strandi32 – 387Combined sources
Helixi41 – 5414Combined sources
Beta strandi59 – 624Combined sources
Helixi65 – 717Combined sources
Beta strandi74 – 796Combined sources
Beta strandi83 – 853Combined sources
Helixi86 – 949Combined sources
Beta strandi97 – 1037Combined sources
Helixi112 – 1154Combined sources
Beta strandi116 – 12510Combined sources
Helixi129 – 14012Combined sources
Turni144 – 1463Combined sources
Beta strandi153 – 1575Combined sources
Beta strandi160 – 1623Combined sources
Beta strandi164 – 1663Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2O3AX-ray2.20A/B2-168[»]
ProteinModelPortaliO29507.
SMRiO29507. Positions 1-167.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO29507.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni104 – 1085S-adenosyl-L-methionine bindingBy similarity

Sequence similaritiesi

Belongs to the aTrm56 family.Curated

Phylogenomic databases

eggNOGiarCOG01857. Archaea.
COG1303. LUCA.
KOiK07254.
OMAiLGHRPER.

Family and domain databases

Gene3Di3.40.1280.10. 1 hit.
HAMAPiMF_00077. tRNA_methyltr_aTrm56.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR029026. tRNA_m1G_MTases_N.
IPR002845. tRNA_mtfrase_aTrm56.
[Graphical view]
PfamiPF01994. Trm56. 1 hit.
[Graphical view]
PIRSFiPIRSF016123. UCP016123. 1 hit.
ProDomiPD016584. DUF127. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF75217. SSF75217. 1 hit.

Sequencei

Sequence statusi: Complete.

O29507-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVYVLRLGH RPERDKRIST HVALTARAFG AKGIYFDTED KSVFESVRDV
60 70 80 90 100
VERWGGDFFI KAVSWKKLLR EFDGLKVHLT MYGIPLPQKL EEIKRADKVL
110 120 130 140 150
VVVGAEKVPP EVYELCDLNI SIGTQPHSEV AALAVFLDRV LGKVFDISFD
160
DAKIKVIPSE RGKRVVSE
Length:168
Mass (Da):19,026
Last modified:January 1, 1998 - v1
Checksum:iACEDE86A81D3C308
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB90489.1.
PIRiG69343.
RefSeqiWP_010878254.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB90489; AAB90489; AF_0751.
GeneIDi24794349.
KEGGiafu:AF_0751.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB90489.1.
PIRiG69343.
RefSeqiWP_010878254.1. NC_000917.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2O3AX-ray2.20A/B2-168[»]
ProteinModelPortaliO29507.
SMRiO29507. Positions 1-167.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224325.AF0751.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB90489; AAB90489; AF_0751.
GeneIDi24794349.
KEGGiafu:AF_0751.

Phylogenomic databases

eggNOGiarCOG01857. Archaea.
COG1303. LUCA.
KOiK07254.
OMAiLGHRPER.

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-767-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO29507.

Family and domain databases

Gene3Di3.40.1280.10. 1 hit.
HAMAPiMF_00077. tRNA_methyltr_aTrm56.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR029026. tRNA_m1G_MTases_N.
IPR002845. tRNA_mtfrase_aTrm56.
[Graphical view]
PfamiPF01994. Trm56. 1 hit.
[Graphical view]
PIRSFiPIRSF016123. UCP016123. 1 hit.
ProDomiPD016584. DUF127. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF75217. SSF75217. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus."
    Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G.
    , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C.
    Nature 390:364-370(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.
  2. "Crystal structure of a protein AF_0751 from Archaeoglobus fulgidus."
    New York structural genomix research consortium (NYSGXRC)
    Submitted (DEC-2006) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS), SUBUNIT.

Entry informationi

Entry nameiTRM56_ARCFU
AccessioniPrimary (citable) accession number: O29507
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: November 11, 2015
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.