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Protein

Tyrosine--tRNA ligase

Gene

tyrS

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).UniRule annotation

Catalytic activityi

ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36TyrosineUniRule annotation1 Publication1
Binding sitei158TyrosineUniRule annotation1 Publication1
Binding sitei162TyrosineUniRule annotation1 Publication1
Binding sitei165TyrosineUniRule annotation1 Publication1
Binding sitei180TyrosineUniRule annotation1 Publication1
Binding sitei217ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.1.1.1. 414.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine--tRNA ligaseUniRule annotation (EC:6.1.1.1UniRule annotation)
Alternative name(s):
Tyrosyl-tRNA synthetaseUniRule annotation
Short name:
TyrRSUniRule annotation
Gene namesi
Name:tyrSUniRule annotation
Ordered Locus Names:AF_0776
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000556681 – 323Tyrosine--tRNA ligaseAdd BLAST323

Interactioni

Subunit structurei

Homodimer.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi224325.AF0776.

Structurei

Secondary structure

1323
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 11Combined sources9
Beta strandi15 – 18Combined sources4
Helixi20 – 28Combined sources9
Beta strandi34 – 39Combined sources6
Helixi47 – 61Combined sources15
Beta strandi65 – 70Combined sources6
Helixi72 – 77Combined sources6
Helixi83 – 99Combined sources17
Turni104 – 106Combined sources3
Beta strandi108 – 111Combined sources4
Helixi112 – 114Combined sources3
Turni115 – 117Combined sources3
Helixi119 – 131Combined sources13
Helixi134 – 140Combined sources7
Turni141 – 144Combined sources4
Beta strandi148 – 150Combined sources3
Helixi154 – 168Combined sources15
Beta strandi172 – 177Combined sources6
Helixi178 – 180Combined sources3
Helixi181 – 190Combined sources10
Helixi191 – 194Combined sources4
Beta strandi200 – 204Combined sources5
Beta strandi212 – 214Combined sources3
Turni217 – 220Combined sources4
Helixi229 – 237Combined sources9
Helixi250 – 257Combined sources8
Helixi259 – 263Combined sources5
Beta strandi266 – 268Combined sources3
Helixi272 – 274Combined sources3
Beta strandi278 – 282Combined sources5
Helixi283 – 291Combined sources9
Helixi297 – 319Combined sources23

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CYBX-ray1.80A/B1-323[»]
ProteinModelPortaliO29482.
SMRiO29482.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO29482.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi41 – 49"HIGH" region9
Motifi214 – 218"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 3 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01886. Archaea.
COG0162. LUCA.
KOiK01866.
OMAiFSKLIYP.

Family and domain databases

CDDicd00805. TyrRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_02008. Tyr_tRNA_synth_type3. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002307. Tyr-tRNA-ligase.
IPR023684. Tyr-tRNA-ligase_3.
IPR023617. Tyr-tRNA-ligase_arc/euk-type.
[Graphical view]
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PIRSFiPIRSF006588. TyrRS_arch_euk. 1 hit.
PRINTSiPR01040. TRNASYNTHTYR.
TIGRFAMsiTIGR00234. tyrS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O29482-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDITEKLRLI TRNAEEVVTE EELRQLIETK EKPRAYVGYE PSGEIHLGHM
60 70 80 90 100
MTVQKLMDLQ EAGFEIIVLL ADIHAYLNEK GTFEEIAEVA DYNKKVFIAL
110 120 130 140 150
GLDESRAKFV LGSEYQLSRD YVLDVLKMAR ITTLNRARRS MDEVSRRKED
160 170 180 190 200
PMVSQMIYPL MQALDIAHLG VDLAVGGIDQ RKIHMLAREN LPRLGYSSPV
210 220 230 240 250
CLHTPILVGL DGQKMSSSKG NYISVRDPPE EVERKIRKAY CPAGVVEENP
260 270 280 290 300
ILDIAKYHIL PRFGKIVVER DAKFGGDVEY ASFEELAEDF KSGQLHPLDL
310 320
KIAVAKYLNM LLEDARKRLG VSV
Length:323
Mass (Da):36,616
Last modified:January 1, 1998 - v1
Checksum:iA655AEE4A51166A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB90462.1.
PIRiH69346.
RefSeqiWP_010878279.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB90462; AAB90462; AF_0776.
GeneIDi24794373.
KEGGiafu:AF_0776.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB90462.1.
PIRiH69346.
RefSeqiWP_010878279.1. NC_000917.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CYBX-ray1.80A/B1-323[»]
ProteinModelPortaliO29482.
SMRiO29482.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224325.AF0776.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB90462; AAB90462; AF_0776.
GeneIDi24794373.
KEGGiafu:AF_0776.

Phylogenomic databases

eggNOGiarCOG01886. Archaea.
COG0162. LUCA.
KOiK01866.
OMAiFSKLIYP.

Enzyme and pathway databases

BRENDAi6.1.1.1. 414.

Miscellaneous databases

EvolutionaryTraceiO29482.

Family and domain databases

CDDicd00805. TyrRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_02008. Tyr_tRNA_synth_type3. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002307. Tyr-tRNA-ligase.
IPR023684. Tyr-tRNA-ligase_3.
IPR023617. Tyr-tRNA-ligase_arc/euk-type.
[Graphical view]
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PIRSFiPIRSF006588. TyrRS_arch_euk. 1 hit.
PRINTSiPR01040. TRNASYNTHTYR.
TIGRFAMsiTIGR00234. tyrS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYY_ARCFU
AccessioniPrimary (citable) accession number: O29482
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: November 30, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.