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Protein

Tyrosine--tRNA ligase

Gene

tyrS

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).UniRule annotation

Catalytic activityi

ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei36 – 361TyrosineUniRule annotation1 Publication
Binding sitei158 – 1581TyrosineUniRule annotation1 Publication
Binding sitei162 – 1621TyrosineUniRule annotation1 Publication
Binding sitei165 – 1651TyrosineUniRule annotation1 Publication
Binding sitei180 – 1801TyrosineUniRule annotation1 Publication
Binding sitei217 – 2171ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-792-MONOMER.
BRENDAi6.1.1.1. 414.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine--tRNA ligaseUniRule annotation (EC:6.1.1.1UniRule annotation)
Alternative name(s):
Tyrosyl-tRNA synthetaseUniRule annotation
Short name:
TyrRSUniRule annotation
Gene namesi
Name:tyrSUniRule annotation
Ordered Locus Names:AF_0776
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 323323Tyrosine--tRNA ligasePRO_0000055668Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi224325.AF0776.

Structurei

Secondary structure

1
323
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 119Combined sources
Beta strandi15 – 184Combined sources
Helixi20 – 289Combined sources
Beta strandi34 – 396Combined sources
Helixi47 – 6115Combined sources
Beta strandi65 – 706Combined sources
Helixi72 – 776Combined sources
Helixi83 – 9917Combined sources
Turni104 – 1063Combined sources
Beta strandi108 – 1114Combined sources
Helixi112 – 1143Combined sources
Turni115 – 1173Combined sources
Helixi119 – 13113Combined sources
Helixi134 – 1407Combined sources
Turni141 – 1444Combined sources
Beta strandi148 – 1503Combined sources
Helixi154 – 16815Combined sources
Beta strandi172 – 1776Combined sources
Helixi178 – 1803Combined sources
Helixi181 – 19010Combined sources
Helixi191 – 1944Combined sources
Beta strandi200 – 2045Combined sources
Beta strandi212 – 2143Combined sources
Turni217 – 2204Combined sources
Helixi229 – 2379Combined sources
Helixi250 – 2578Combined sources
Helixi259 – 2635Combined sources
Beta strandi266 – 2683Combined sources
Helixi272 – 2743Combined sources
Beta strandi278 – 2825Combined sources
Helixi283 – 2919Combined sources
Helixi297 – 31923Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CYBX-ray1.80A/B1-323[»]
ProteinModelPortaliO29482.
SMRiO29482. Positions 2-320.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO29482.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi41 – 499"HIGH" region
Motifi214 – 2185"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 3 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01886. Archaea.
COG0162. LUCA.
KOiK01866.
OMAiFSKLIYP.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_02008. Tyr_tRNA_synth_type3. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002307. Tyr-tRNA-ligase.
IPR023684. Tyr-tRNA-ligase_3.
IPR023617. Tyr-tRNA-ligase_arc/euk-type.
[Graphical view]
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PIRSFiPIRSF006588. TyrRS_arch_euk. 1 hit.
PRINTSiPR01040. TRNASYNTHTYR.
TIGRFAMsiTIGR00234. tyrS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O29482-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDITEKLRLI TRNAEEVVTE EELRQLIETK EKPRAYVGYE PSGEIHLGHM
60 70 80 90 100
MTVQKLMDLQ EAGFEIIVLL ADIHAYLNEK GTFEEIAEVA DYNKKVFIAL
110 120 130 140 150
GLDESRAKFV LGSEYQLSRD YVLDVLKMAR ITTLNRARRS MDEVSRRKED
160 170 180 190 200
PMVSQMIYPL MQALDIAHLG VDLAVGGIDQ RKIHMLAREN LPRLGYSSPV
210 220 230 240 250
CLHTPILVGL DGQKMSSSKG NYISVRDPPE EVERKIRKAY CPAGVVEENP
260 270 280 290 300
ILDIAKYHIL PRFGKIVVER DAKFGGDVEY ASFEELAEDF KSGQLHPLDL
310 320
KIAVAKYLNM LLEDARKRLG VSV
Length:323
Mass (Da):36,616
Last modified:January 1, 1998 - v1
Checksum:iA655AEE4A51166A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB90462.1.
PIRiH69346.
RefSeqiWP_010878279.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB90462; AAB90462; AF_0776.
GeneIDi24794373.
KEGGiafu:AF_0776.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB90462.1.
PIRiH69346.
RefSeqiWP_010878279.1. NC_000917.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CYBX-ray1.80A/B1-323[»]
ProteinModelPortaliO29482.
SMRiO29482. Positions 2-320.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224325.AF0776.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB90462; AAB90462; AF_0776.
GeneIDi24794373.
KEGGiafu:AF_0776.

Phylogenomic databases

eggNOGiarCOG01886. Archaea.
COG0162. LUCA.
KOiK01866.
OMAiFSKLIYP.

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-792-MONOMER.
BRENDAi6.1.1.1. 414.

Miscellaneous databases

EvolutionaryTraceiO29482.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_02008. Tyr_tRNA_synth_type3. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002307. Tyr-tRNA-ligase.
IPR023684. Tyr-tRNA-ligase_3.
IPR023617. Tyr-tRNA-ligase_arc/euk-type.
[Graphical view]
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PIRSFiPIRSF006588. TyrRS_arch_euk. 1 hit.
PRINTSiPR01040. TRNASYNTHTYR.
TIGRFAMsiTIGR00234. tyrS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYY_ARCFU
AccessioniPrimary (citable) accession number: O29482
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.