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O29482 (SYY_ARCFU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Tyrosine--tRNA ligase
EC=6.1.1.1
Alternative name(s):
Tyrosyl-tRNA synthetase
Short name=TyrRS
Gene names
Name:tyrS
Ordered Locus Names:AF_0776
OrganismArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifier224325 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length323 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity. HAMAP MF_02008

Catalytic activity

ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP MF_02008

Subunit structure

Homodimer. Ref.2

Subcellular location

Cytoplasm HAMAP MF_02008.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 3 subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological processtyrosyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

tyrosine-tRNA ligase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 323323Tyrosine--tRNA ligase HAMAP MF_02008
PRO_0000055668

Regions

Motif41 – 499"HIGH" region HAMAP MF_02008
Motif214 – 2185"KMSKS" region HAMAP MF_02008

Sites

Binding site361Tyrosine
Binding site1581Tyrosine
Binding site1621Tyrosine
Binding site1651Tyrosine
Binding site1801Tyrosine
Binding site2171ATP Potential

Secondary structure

1 .......................................................... 323
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O29482 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: A655AEE4A51166A2

FASTA32336,616
        10         20         30         40         50         60 
MDITEKLRLI TRNAEEVVTE EELRQLIETK EKPRAYVGYE PSGEIHLGHM MTVQKLMDLQ 

        70         80         90        100        110        120 
EAGFEIIVLL ADIHAYLNEK GTFEEIAEVA DYNKKVFIAL GLDESRAKFV LGSEYQLSRD 

       130        140        150        160        170        180 
YVLDVLKMAR ITTLNRARRS MDEVSRRKED PMVSQMIYPL MQALDIAHLG VDLAVGGIDQ 

       190        200        210        220        230        240 
RKIHMLAREN LPRLGYSSPV CLHTPILVGL DGQKMSSSKG NYISVRDPPE EVERKIRKAY 

       250        260        270        280        290        300 
CPAGVVEENP ILDIAKYHIL PRFGKIVVER DAKFGGDVEY ASFEELAEDF KSGQLHPLDL 

       310        320 
KIAVAKYLNM LLEDARKRLG VSV

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE000782 Genomic DNA. Translation: AAB90462.1.
PIRH69346.
RefSeqNP_069610.1. NC_000917.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2CYBX-ray1.80A/B1-323[»]
ProteinModelPortalO29482.
SMRO29482. Positions 2-320.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1483993.
GenomeReviewsGene locus AF_0776 in contig AE000782_GR.
KEGGafu:AF0776.
NMPDRfig|224325.1.peg.769.
TIGRAF_0776.

Phylogenomic databases

HOGENOMHBG330667.
OMAGMEQRKI.
PhylomeDBO29482.
ProtClustDBPRK08560.

Enzyme and pathway databases

BioCycAFUL224325:AF_0776-MONOMER.

Family and domain databases

HAMAPMF_02008. Tyr_tRNA_synth_type3.
[Tree]
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002307. Tyr-tRNA-synth.
IPR023684. Tyr-tRNA-synth_3.
IPR023617. Tyr-tRNA-synth_arc-type.
[Graphical view]
Gene3DG3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KOK01866.
PfamPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PIRSFPIRSF006588. TyrRS_arch_euk. 1 hit.
PRINTSPR01040. TRNASYNTHTYR.
TIGRFAMsTIGR00234. TyrS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYY_ARCFU
AccessionPrimary (citable) accession number: O29482
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: January 25, 2012
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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