O29417 (PURA_ARCFU) Reviewed, UniProtKB/Swiss-Prot
Last modified
November 16, 2011.
Version 84.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Adenylosuccinate synthetase Short name=AMPSase Short name=AdSS EC=6.3.4.4 Alternative name(s): IMP--aspartate ligase | ||||
| Gene names |
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| Organism | Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) | ||||
| Taxonomic identifier | 224325 [NCBI] | ||||
| Taxonomic lineage | Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus |
Protein attributes
| Sequence length | 337 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP By similarity. HAMAP MF_00011 |
| Catalytic activity | GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. HAMAP MF_00011 |
| Cofactor | Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00011 |
| Pathway | Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. HAMAP MF_00011 |
| Subunit structure | Homodimer By similarity. HAMAP MF_00011 |
| Subcellular location | Cytoplasm By similarity HAMAP MF_00011. |
| Sequence similarities | Belongs to the adenylosuccinate synthetase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Purine biosynthesis |
| Cellular component | Cytoplasm |
| Ligand | GTP-binding Magnesium Metal-binding Nucleotide-binding |
| Molecular function | Ligase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | GTP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylosuccinate synthase activityInferred from electronic annotation. Source: EC magnesium ion bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 337 | 337 | Adenylosuccinate synthetase HAMAP MF_00011 | PRO_0000095266 | |||||
Regions | |||||||||
| Nucleotide binding | 12 – 18 | 7 | GTP By similarity | ||||||
| Nucleotide binding | 42 – 44 | 3 | GTP By similarity | ||||||
| Nucleotide binding | 281 – 283 | 3 | GTP By similarity | ||||||
| Nucleotide binding | 321 – 323 | 3 | GTP By similarity | ||||||
| Region | 13 – 16 | 4 | IMP binding By similarity | ||||||
| Region | 40 – 43 | 4 | IMP binding By similarity | ||||||
| Region | 249 – 255 | 7 | Substrate binding By similarity | ||||||
Sites | |||||||||
| Active site | 13 | 1 | Proton acceptor By similarity | ||||||
| Active site | 43 | 1 | Proton donor By similarity | ||||||
| Metal binding | 13 | 1 | Magnesium By similarity | ||||||
| Metal binding | 42 | 1 | Magnesium; via carbonyl oxygen By similarity | ||||||
| Binding site | 124 | 1 | IMP By similarity | ||||||
| Binding site | 138 | 1 | IMP; shared with dimeric partner By similarity | ||||||
| Binding site | 176 | 1 | IMP By similarity | ||||||
| Binding site | 191 | 1 | IMP By similarity | ||||||
| Binding site | 253 | 1 | IMP By similarity | ||||||
| Binding site | 255 | 1 | GTP By similarity | ||||||
Sequences
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References
| [1] | "The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. Venter J.C.Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AE000782 Genomic DNA. Translation: AAB90398.1. |
| PIR | A69355. |
| RefSeq | NP_069675.1. NC_000917.1. |
3D structure databases | |
| ProteinModelPortal | O29417. |
| SMR | O29417. Positions 1-333. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 1484060. |
| GenomeReviews | Gene locus AF_0841 in contig AE000782_GR. |
| KEGG | afu:AF0841. |
| NMPDR | fig|224325.1.peg.834. |
| TIGR | AF_0841. |
Phylogenomic databases | |
| HOGENOM | HBG658237. |
| OMA | IDPHVGI. |
| ProtClustDB | PRK04293. |
Enzyme and pathway databases | |
| BioCyc | AFUL224325:AF_0841-MONOMER. |
Family and domain databases | |
| HAMAP | MF_00011. Adenylosucc_synth. [Tree] |
| InterPro | IPR018220. Adenylosuccinate_synthase_AS. IPR001114. Adenylosuccinate_synthetase. [Graphical view] |
| KO | K01939. |
| PANTHER | PTHR11846. Asucc_synthtase. 1 hit. |
| Pfam | PF00709. Adenylsucc_synt. 3 hits. [Graphical view] |
| SMART | SM00788. Adenylsucc_synt. 1 hit. [Graphical view] |
| PROSITE | PS01266. ADENYLOSUCCIN_SYN_1. 1 hit. PS00513. ADENYLOSUCCIN_SYN_2. False negative. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | PURA_ARCFU | ||||||||
| Accession | Primary (citable) accession number: O29417 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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