Reviewed, UniProtKB/Swiss-Prot O29388 (PUR1_ARCFU)

Names and origin

Protein names

Recommended name:
    Probable amidophosphoribosyltransferase
      Short name=ATase
    EC=2.4.2.14
Alternative name(s):
    Glutamine phosphoribosylpyrophosphate amidotransferase
      Short name=GPATase

Gene names

Name:	purF
Ordered Locus Names:	AF_0873

Organism

Archaeoglobus fulgidus [Complete proteome] [HAMAP]

Taxonomic identifier

2234 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	457 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.
Cofactor	Binds 1 magnesium ion per subunit By similarity. Binds 1 4Fe-4S cluster per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.
Sequence similarities	In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. Contains 1 glutamine amidotransferase type-2 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	4Fe-4S Iron Iron-sulfur Magnesium Metal-binding
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW nucleoside metabolic process Inferred from electronic annotation. Source: InterPro purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW amidophosphoribosyltransferase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 457	456	Probable amidophosphoribosyltransferase		PRO_0000139643
Regions
	Domain	2 – 223	222	Glutamine amidotransferase type-2
Sites
	Active site	2	1	For GATase activity By similarity
	Metal binding	239	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	286	1	Magnesium By similarity
	Metal binding	348	1	Magnesium By similarity
	Metal binding	349	1	Magnesium By similarity
	Metal binding	385	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	438	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	441	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	O29388-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 8F069D6778257154	FASTA	457	50,342
	10 20 30 40 50 60 MCGVVGIYHP DGELAPRLAY YSLFSLQHRG QESAGIASFD NHIRQKRGMG LVTEVFNDED 70 80 90 100 110 120 FELLAGKSVI GHVRYSTTGR SRLENAQPFV VKSKAGYIAV AHNGNLVNYS QLRNELENEG 130 140 150 160 170 180 RVFTTDSDTE VISQLLSKFL IEEGDIINAL ERLNESLVGS YTMTMLVDDA VIGYRDPLGF 190 200 210 220 230 240 KPLCVGRIDD GYVICSESCA IDALGGEFIR DVQPGKAAII KDGELEFVKI AKSERRAVCI 250 260 270 280 290 300 FEYIYFARPD SIIDGISVYK ARSEMGKVLA RESPVEADFV SAVPDSGITA AIGYAQESGL 310 320 330 340 350 360 PYFEGLIKNR YVGRTFIMPV QSLRETSVRL KVNVVRENVR GRRVVLVDDS IVRGTTSRRI 370 380 390 400 410 420 VQMIKDAGAK EVHMRIGSPP IIAPCYFGID MKSREELIAA SHTVEEIGRI FGTDSLAYLS 430 440 450 LEGLLEAVRR AGGKRGYCLA CLTSKYPVSV PGEECEC

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000782 Genomic DNA. Translation: AAB90366.1.
PIR	A69359.
RefSeq	NP_069707.1.
3D structure databases
HSSP	HSSP built from PDB template 1AO0 based on UniProtKB P00497.
ModBase	Search...
Genome annotation databases
GeneID	1484093.
GenomeReviews	Gene locus AF_0873 in contig AE000782_GR.
KEGG	afu:AF0873.
NMPDR	fig|224325.1.peg.866.
TIGR	AF_0873.
Phylogenomic databases
HOGENOM	O29388.
OMA	IVPINAG.
Enzyme and pathway databases
BioCyc	AFUL224325:AF_0873-MON.
BRENDA	2.4.2.14. 7576.
Family and domain databases
InterPro	IPR005854. Amd_phspho_trans. IPR000583. GATase_2. IPR017932. GATase_II. IPR002375. Pr/py_Pribosyl_transf_CS. IPR000836. PRibTrfase. [Graphical view]
PANTHER	PTHR11907. Amd_phspho_trans. 1 hit.
Pfam	PF00310. GATase_2. 1 hit. PF00156. Pribosyltran. 1 hit. [Graphical view]
PIRSF	PIRSF000485. Amd_phspho_trans. 1 hit.
TIGRFAMs	TIGR01134. purF. 1 hit.
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR1_ARCFU

Accession

Primary (citable) accession number: O29388

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 74 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)