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O29388

- PUR1_ARCFU

UniProt

O29388 - PUR1_ARCFU

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Protein

Probable amidophosphoribosyltransferase

Gene
purF, AF_0873
Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactori

Binds 1 magnesium ion per subunit By similarity.
Binds 1 4Fe-4S cluster per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21For GATase activity By similarity
Metal bindingi239 – 2391Iron-sulfur (4Fe-4S) By similarity
Metal bindingi286 – 2861Magnesium By similarity
Metal bindingi348 – 3481Magnesium By similarity
Metal bindingi349 – 3491Magnesium By similarity
Metal bindingi385 – 3851Iron-sulfur (4Fe-4S) By similarity
Metal bindingi438 – 4381Iron-sulfur (4Fe-4S) By similarity
Metal bindingi441 – 4411Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. amidophosphoribosyltransferase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. glutamine metabolic process Source: UniProtKB-KW
  3. nucleoside metabolic process Source: InterPro
  4. purine nucleobase biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-892-MONOMER.
UniPathwayiUPA00074; UER00124.

Protein family/group databases

MEROPSiC44.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable amidophosphoribosyltransferase (EC:2.4.2.14)
Short name:
ATase
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Short name:
GPATase
Gene namesi
Name:purF
Ordered Locus Names:AF_0873
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
ProteomesiUP000002199: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 457456Probable amidophosphoribosyltransferasePRO_0000139643Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi224325.AF0873.

Structurei

3D structure databases

ProteinModelPortaliO29388.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 223222Glutamine amidotransferase type-2Add
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0034.
KOiK00764.
OMAiSGIPYEM.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O29388-1 [UniParc]FASTAAdd to Basket

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MCGVVGIYHP DGELAPRLAY YSLFSLQHRG QESAGIASFD NHIRQKRGMG    50
LVTEVFNDED FELLAGKSVI GHVRYSTTGR SRLENAQPFV VKSKAGYIAV 100
AHNGNLVNYS QLRNELENEG RVFTTDSDTE VISQLLSKFL IEEGDIINAL 150
ERLNESLVGS YTMTMLVDDA VIGYRDPLGF KPLCVGRIDD GYVICSESCA 200
IDALGGEFIR DVQPGKAAII KDGELEFVKI AKSERRAVCI FEYIYFARPD 250
SIIDGISVYK ARSEMGKVLA RESPVEADFV SAVPDSGITA AIGYAQESGL 300
PYFEGLIKNR YVGRTFIMPV QSLRETSVRL KVNVVRENVR GRRVVLVDDS 350
IVRGTTSRRI VQMIKDAGAK EVHMRIGSPP IIAPCYFGID MKSREELIAA 400
SHTVEEIGRI FGTDSLAYLS LEGLLEAVRR AGGKRGYCLA CLTSKYPVSV 450
PGEECEC 457
Length:457
Mass (Da):50,342
Last modified:January 23, 2007 - v3
Checksum:i8F069D6778257154
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000782 Genomic DNA. Translation: AAB90366.1.
PIRiA69359.
RefSeqiNP_069707.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB90366; AAB90366; AF_0873.
GeneIDi1484093.
KEGGiafu:AF0873.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000782 Genomic DNA. Translation: AAB90366.1 .
PIRi A69359.
RefSeqi NP_069707.1. NC_000917.1.

3D structure databases

ProteinModelPortali O29388.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224325.AF0873.

Protein family/group databases

MEROPSi C44.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB90366 ; AAB90366 ; AF_0873 .
GeneIDi 1484093.
KEGGi afu:AF0873.

Phylogenomic databases

eggNOGi COG0034.
KOi K00764.
OMAi SGIPYEM.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00124 .
BioCyci AFUL224325:GJBC-892-MONOMER.

Family and domain databases

Gene3Di 3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
InterProi IPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view ]
Pfami PF00156. Pribosyltran. 1 hit.
[Graphical view ]
PIRSFi PIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMi SSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01134. purF. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus."
    Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G.
    , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C.
    Nature 390:364-370(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

Entry informationi

Entry nameiPUR1_ARCFU
AccessioniPrimary (citable) accession number: O29388
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 103 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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