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O29388 (PUR1_ARCFU) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable amidophosphoribosyltransferase

Short name=ATase
EC=2.4.2.14
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Short name=GPATase
Gene names
Name:purF
Ordered Locus Names:AF_0873
OrganismArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) [Reference proteome] [HAMAP]
Taxonomic identifier224325 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus

Protein attributes

Sequence length457 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Binds 1 4Fe-4S cluster per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.

Sequence similarities

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

Contains 1 glutamine amidotransferase type-2 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 457456Probable amidophosphoribosyltransferase
PRO_0000139643

Regions

Domain2 – 223222Glutamine amidotransferase type-2

Sites

Active site21For GATase activity By similarity
Metal binding2391Iron-sulfur (4Fe-4S) By similarity
Metal binding2861Magnesium By similarity
Metal binding3481Magnesium By similarity
Metal binding3491Magnesium By similarity
Metal binding3851Iron-sulfur (4Fe-4S) By similarity
Metal binding4381Iron-sulfur (4Fe-4S) By similarity
Metal binding4411Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
O29388 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 8F069D6778257154

FASTA45750,342
        10         20         30         40         50         60 
MCGVVGIYHP DGELAPRLAY YSLFSLQHRG QESAGIASFD NHIRQKRGMG LVTEVFNDED 

        70         80         90        100        110        120 
FELLAGKSVI GHVRYSTTGR SRLENAQPFV VKSKAGYIAV AHNGNLVNYS QLRNELENEG 

       130        140        150        160        170        180 
RVFTTDSDTE VISQLLSKFL IEEGDIINAL ERLNESLVGS YTMTMLVDDA VIGYRDPLGF 

       190        200        210        220        230        240 
KPLCVGRIDD GYVICSESCA IDALGGEFIR DVQPGKAAII KDGELEFVKI AKSERRAVCI 

       250        260        270        280        290        300 
FEYIYFARPD SIIDGISVYK ARSEMGKVLA RESPVEADFV SAVPDSGITA AIGYAQESGL 

       310        320        330        340        350        360 
PYFEGLIKNR YVGRTFIMPV QSLRETSVRL KVNVVRENVR GRRVVLVDDS IVRGTTSRRI 

       370        380        390        400        410        420 
VQMIKDAGAK EVHMRIGSPP IIAPCYFGID MKSREELIAA SHTVEEIGRI FGTDSLAYLS 

       430        440        450 
LEGLLEAVRR AGGKRGYCLA CLTSKYPVSV PGEECEC 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000782 Genomic DNA. Translation: AAB90366.1.
PIRA69359.
RefSeqNP_069707.1. NC_000917.1.

3D structure databases

ProteinModelPortalO29388.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224325.AF0873.

Protein family/group databases

MEROPSC44.001.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB90366; AAB90366; AF_0873.
GeneID1484093.
KEGGafu:AF0873.

Phylogenomic databases

eggNOGCOG0034.
KOK00764.
OMACDACFTG.
ProtClustDBCLSK2746637.

Enzyme and pathway databases

BioCycAFUL224325:GJBC-892-MONOMER.
UniPathwayUPA00074; UER00124.

Family and domain databases

InterProIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000836. PRibTrfase_dom.
[Graphical view]
PfamPF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFPIRSF000485. Amd_phspho_trans. 1 hit.
TIGRFAMsTIGR01134. purF. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR1_ARCFU
AccessionPrimary (citable) accession number: O29388
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: October 16, 2013
This is version 101 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways