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Protein

Glutamyl-tRNA(Gln) amidotransferase subunit D

Gene

gatD

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The GatDE system is specific for glutamate and does not act on aspartate.UniRule annotation

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei91UniRule annotation1
Active sitei166UniRule annotation1
Active sitei167UniRule annotation1
Active sitei243UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAFUL224325:G1G17-910-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit DUniRule annotation (EC:6.3.5.-UniRule annotation)
Short name:
Glu-ADT subunit DUniRule annotation
Gene namesi
Name:gatDUniRule annotation
Ordered Locus Names:AF_0882
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001400491 – 418Glutamyl-tRNA(Gln) amidotransferase subunit DAdd BLAST418

Interactioni

Subunit structurei

Heterodimer of GatD and GatE.UniRule annotation

Protein-protein interaction databases

STRINGi224325.AF0882

Structurei

3D structure databases

ProteinModelPortaliO29380
SMRiO29380
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini81 – 407Asparaginase/glutaminasePROSITE-ProRule annotationAdd BLAST327

Sequence similaritiesi

Belongs to the asparaginase 1 family. GatD subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01924 Archaea
COG0252 LUCA
KOiK09482
OMAiNVYSTGR
OrthoDBiPOG093Z07VD

Family and domain databases

CDDicd08962 GatD, 1 hit
Gene3Di2.30.30.520, 1 hit
3.40.50.1170, 1 hit
3.40.50.40, 1 hit
HAMAPiMF_00586 GatD, 1 hit
InterProiView protein in InterPro
IPR006033 AsnASEI
IPR036152 Asp/glu_Ase-like_sf
IPR006034 Asparaginase/glutaminase-like
IPR020827 Asparaginase/glutaminase_AS1
IPR027475 Asparaginase/glutaminase_AS2
IPR011878 GatD
IPR037222 GatD_N
IPR027473 L-asparaginase_C
IPR027474 L-asparaginase_N
IPR037152 L-asparaginase_N_sf
PfamiView protein in Pfam
PF00710 Asparaginase, 1 hit
PIRSFiPIRSF500175 Glu_ADT_D, 1 hit
PIRSF001220 L-ASNase_gatD, 1 hit
PRINTSiPR00139 ASNGLNASE
SMARTiView protein in SMART
SM00870 Asparaginase, 1 hit
SUPFAMiSSF141300 SSF141300, 1 hit
SSF53774 SSF53774, 1 hit
TIGRFAMsiTIGR00519 asnASE_I, 1 hit
TIGR02153 gatD_arch, 1 hit
PROSITEiView protein in PROSITE
PS00144 ASN_GLN_ASE_1, 1 hit
PS00917 ASN_GLN_ASE_2, 1 hit
PS51732 ASN_GLN_ASE_3, 1 hit

Sequencei

Sequence statusi: Complete.

O29380-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPKSWGWIS MSERLEGKRV RIKAKGKIFE GIVMPSFTGN FVLKLDNGYN
60 70 80 90 100
VGFKEYELLE VLEVEPFEPH LPELVKREGL PDVKIISTGG TIASKVDYRT
110 120 130 140 150
GAVTSQFTAE EIASEVPELT EICNVDAELL YNILSENMKP ENWIELARHV
160 170 180 190 200
YKALKDHEGV IITHGTDTMH FSAAALSFML STPKPVVFVG AQRSSDRPSS
210 220 230 240 250
DAAMNLLCAA KAATEDIGEV VVCMHGSTSD DYCLVHRGVK VRKNHTSRRD
260 270 280 290 300
AFQSVNAKPI GRIDYPSLSV EWLSWRYRRG ERELKLTDRL ERKVVLIKFF
310 320 330 340 350
PGLSSDILEY YHSKGYRGFV IEGTGLGHVS TDWIDTLRRV CEDSVVVMTS
360 370 380 390 400
QCLWGRVCDR VYDTGRDILR AGVIEGEDML PEVALIKLMW LLGNYSIEEA
410
KEMVKKSVAG EIEPTTQY
Length:418
Mass (Da):47,091
Last modified:January 1, 1998 - v1
Checksum:iC3F4A4AE831DD05D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA Translation: AAB90360.1
PIRiB69360
RefSeqiWP_010878382.1, NC_000917.1

Genome annotation databases

EnsemblBacteriaiAAB90360; AAB90360; AF_0882
GeneIDi1484104
KEGGiafu:AF_0882

Similar proteinsi

Entry informationi

Entry nameiGATD_ARCFU
AccessioniPrimary (citable) accession number: O29380
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2002
Last sequence update: January 1, 1998
Last modified: March 28, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health