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Protein

Glutamyl-tRNA(Gln) amidotransferase subunit D

Gene

gatD

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The GatDE system is specific for glutamate and does not act on aspartate.UniRule annotation

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei91UniRule annotation1
Active sitei166UniRule annotation1
Active sitei167UniRule annotation1
Active sitei243UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit DUniRule annotation (EC:6.3.5.-UniRule annotation)
Short name:
Glu-ADT subunit DUniRule annotation
Gene namesi
Name:gatDUniRule annotation
Ordered Locus Names:AF_0882
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001400491 – 418Glutamyl-tRNA(Gln) amidotransferase subunit DAdd BLAST418

Interactioni

Subunit structurei

Heterodimer of GatD and GatE.UniRule annotation

Protein-protein interaction databases

STRINGi224325.AF0882.

Structurei

3D structure databases

ProteinModelPortaliO29380.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini81 – 407Asparaginase/glutaminasePROSITE-ProRule annotationAdd BLAST327

Sequence similaritiesi

Belongs to the asparaginase 1 family. GatD subfamily.UniRule annotation
Contains 1 asparaginase/glutaminase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiarCOG01924. Archaea.
COG0252. LUCA.
KOiK09482.
OMAiMHGETGD.

Family and domain databases

CDDicd08962. GatD. 1 hit.
Gene3Di3.40.50.1170. 1 hit.
3.40.50.40. 1 hit.
HAMAPiMF_00586. GatD. 1 hit.
InterProiIPR006033. AsnASEI.
IPR006034. Asparaginase/glutaminase.
IPR020827. Asparaginase/glutaminase_AS1.
IPR027475. Asparaginase/glutaminase_AS2.
IPR011878. GatD.
IPR027473. L-asparaginase_C.
IPR027474. L-asparaginase_N.
[Graphical view]
PfamiPF00710. Asparaginase. 1 hit.
[Graphical view]
PIRSFiPIRSF500175. Glu_ADT_D. 1 hit.
PIRSF001220. L-ASNase_gatD. 1 hit.
PRINTSiPR00139. ASNGLNASE.
SMARTiSM00870. Asparaginase. 1 hit.
[Graphical view]
SUPFAMiSSF53774. SSF53774. 1 hit.
TIGRFAMsiTIGR00519. asnASE_I. 1 hit.
TIGR02153. gatD_arch. 1 hit.
PROSITEiPS00144. ASN_GLN_ASE_1. 1 hit.
PS00917. ASN_GLN_ASE_2. 1 hit.
PS51732. ASN_GLN_ASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O29380-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPKSWGWIS MSERLEGKRV RIKAKGKIFE GIVMPSFTGN FVLKLDNGYN
60 70 80 90 100
VGFKEYELLE VLEVEPFEPH LPELVKREGL PDVKIISTGG TIASKVDYRT
110 120 130 140 150
GAVTSQFTAE EIASEVPELT EICNVDAELL YNILSENMKP ENWIELARHV
160 170 180 190 200
YKALKDHEGV IITHGTDTMH FSAAALSFML STPKPVVFVG AQRSSDRPSS
210 220 230 240 250
DAAMNLLCAA KAATEDIGEV VVCMHGSTSD DYCLVHRGVK VRKNHTSRRD
260 270 280 290 300
AFQSVNAKPI GRIDYPSLSV EWLSWRYRRG ERELKLTDRL ERKVVLIKFF
310 320 330 340 350
PGLSSDILEY YHSKGYRGFV IEGTGLGHVS TDWIDTLRRV CEDSVVVMTS
360 370 380 390 400
QCLWGRVCDR VYDTGRDILR AGVIEGEDML PEVALIKLMW LLGNYSIEEA
410
KEMVKKSVAG EIEPTTQY
Length:418
Mass (Da):47,091
Last modified:January 1, 1998 - v1
Checksum:iC3F4A4AE831DD05D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB90360.1.
PIRiB69360.
RefSeqiWP_010878382.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB90360; AAB90360; AF_0882.
GeneIDi1484104.
KEGGiafu:AF_0882.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB90360.1.
PIRiB69360.
RefSeqiWP_010878382.1. NC_000917.1.

3D structure databases

ProteinModelPortaliO29380.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224325.AF0882.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB90360; AAB90360; AF_0882.
GeneIDi1484104.
KEGGiafu:AF_0882.

Phylogenomic databases

eggNOGiarCOG01924. Archaea.
COG0252. LUCA.
KOiK09482.
OMAiMHGETGD.

Family and domain databases

CDDicd08962. GatD. 1 hit.
Gene3Di3.40.50.1170. 1 hit.
3.40.50.40. 1 hit.
HAMAPiMF_00586. GatD. 1 hit.
InterProiIPR006033. AsnASEI.
IPR006034. Asparaginase/glutaminase.
IPR020827. Asparaginase/glutaminase_AS1.
IPR027475. Asparaginase/glutaminase_AS2.
IPR011878. GatD.
IPR027473. L-asparaginase_C.
IPR027474. L-asparaginase_N.
[Graphical view]
PfamiPF00710. Asparaginase. 1 hit.
[Graphical view]
PIRSFiPIRSF500175. Glu_ADT_D. 1 hit.
PIRSF001220. L-ASNase_gatD. 1 hit.
PRINTSiPR00139. ASNGLNASE.
SMARTiSM00870. Asparaginase. 1 hit.
[Graphical view]
SUPFAMiSSF53774. SSF53774. 1 hit.
TIGRFAMsiTIGR00519. asnASE_I. 1 hit.
TIGR02153. gatD_arch. 1 hit.
PROSITEiPS00144. ASN_GLN_ASE_1. 1 hit.
PS00917. ASN_GLN_ASE_2. 1 hit.
PS51732. ASN_GLN_ASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGATD_ARCFU
AccessioniPrimary (citable) accession number: O29380
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2002
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.