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Protein

F420-dependent NADP reductase

Gene

fno

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible reduction of NADP+ by F420H2. In this reaction the proS hydrogen at C5 of F420 is transferred into the proS position at C4 of NADPH.2 Publications

Catalytic activityi

Reduced coenzyme F420 + NADP+ = oxidized coenzyme F420 + NADPH.1 Publication

Kineticsi

  1. KM=20 µM for reduced coenzyme F420 (at pH 8.0 and 65 degrees Celsius)1 Publication
  2. KM=40 µM for NADP+ (at pH 8.0 and 65 degrees Celsius)1 Publication
  3. KM=10 µM for oxidized coenzyme F420 (at pH 5.5 and 65 degrees Celsius)1 Publication
  4. KM=40 µM for NADPH (at pH 5.5 and 65 degrees Celsius)1 Publication
  1. Vmax=660 µmol/min/mg enzyme for NADP reduction with F420H2 (at pH 5.5 and 80 degrees Celsius)1 Publication

pH dependencei

Optimum pH is 8.0 for NADP reduction with F420H2. Optimum pH is 5.5 for F420 reduction with NADPH.1 Publication

Temperature dependencei

Optimum temperature is 80 degrees Celsius. Is highly thermostable.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei36 – 361NADP1 Publication
Binding sitei72 – 721NADP; via carbonyl oxygen1 Publication
Binding sitei76 – 761NADP1 Publication
Binding sitei98 – 981NADP; via amide nitrogen1 Publication
Binding sitei137 – 1371NADP; via amide nitrogen1 Publication
Binding sitei207 – 2071F420; via amide nitrogen and carbonyl oxygen1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 124NADP1 Publication
Nucleotide bindingi31 – 322NADP1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-914-MONOMER.
BRENDAi1.5.1.40. 414.

Names & Taxonomyi

Protein namesi
Recommended name:
F420-dependent NADP reductase1 Publication (EC:1.5.1.401 Publication)
Alternative name(s):
F420H2:NADP+ oxidoreductase1 Publication
Gene namesi
Name:fno
Ordered Locus Names:AF_0892
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 212212F420-dependent NADP reductasePRO_0000419113Add
BLAST

Interactioni

Subunit structurei

Homodimer.2 Publications

Protein-protein interaction databases

STRINGi224325.AF0892.

Structurei

Secondary structure

1
212
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 65Combined sources
Turni7 – 93Combined sources
Helixi11 – 2111Combined sources
Turni22 – 243Combined sources
Beta strandi26 – 338Combined sources
Helixi34 – 4815Combined sources
Beta strandi53 – 575Combined sources
Helixi58 – 647Combined sources
Beta strandi66 – 705Combined sources
Helixi74 – 8310Combined sources
Helixi85 – 884Combined sources
Beta strandi91 – 955Combined sources
Beta strandi100 – 1034Combined sources
Beta strandi106 – 1094Combined sources
Helixi115 – 1239Combined sources
Beta strandi128 – 1303Combined sources
Helixi137 – 1415Combined sources
Beta strandi149 – 1568Combined sources
Helixi158 – 17013Combined sources
Beta strandi174 – 1818Combined sources
Helixi182 – 1843Combined sources
Helixi185 – 1895Combined sources
Helixi191 – 20212Combined sources
Beta strandi209 – 2113Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JAXX-ray1.80A/B1-212[»]
1JAYX-ray1.65A/B1-212[»]
ProteinModelPortaliO29370.
SMRiO29370. Positions 1-212.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO29370.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG00457. Archaea.
COG2085. LUCA.
KOiK06988.
OMAiRRYKAHA.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR010185. NpdG.
IPR028939. ProC_N.
[Graphical view]
PfamiPF03807. F420_oxidored. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01915. npdG. 1 hit.

Sequencei

Sequence statusi: Complete.

O29370-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVALLGGTG NLGKGLALRL ATLGHEIVVG SRREEKAEAK AAEYRRIAGD
60 70 80 90 100
ASITGMKNED AAEACDIAVL TIPWEHAIDT ARDLKNILRE KIVVSPLVPV
110 120 130 140 150
SRGAKGFTYS SERSAAEIVA EVLESEKVVS ALHTIPAARF ANLDEKFDWD
160 170 180 190 200
VPVCGDDDES KKVVMSLISE IDGLRPLDAG PLSNSRLVES LTPLILNIMR
210
FNGMGELGIK FL
Length:212
Mass (Da):22,865
Last modified:January 1, 1998 - v1
Checksum:i16E2F079C9F471CE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB90348.1.
PIRiD69361.
RefSeqiWP_010878392.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB90348; AAB90348; AF_0892.
GeneIDi1484115.
KEGGiafu:AF_0892.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB90348.1.
PIRiD69361.
RefSeqiWP_010878392.1. NC_000917.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JAXX-ray1.80A/B1-212[»]
1JAYX-ray1.65A/B1-212[»]
ProteinModelPortaliO29370.
SMRiO29370. Positions 1-212.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224325.AF0892.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB90348; AAB90348; AF_0892.
GeneIDi1484115.
KEGGiafu:AF_0892.

Phylogenomic databases

eggNOGiarCOG00457. Archaea.
COG2085. LUCA.
KOiK06988.
OMAiRRYKAHA.

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-914-MONOMER.
BRENDAi1.5.1.40. 414.

Miscellaneous databases

EvolutionaryTraceiO29370.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR010185. NpdG.
IPR028939. ProC_N.
[Graphical view]
PfamiPF03807. F420_oxidored. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01915. npdG. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFNO_ARCFU
AccessioniPrimary (citable) accession number: O29370
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: January 1, 1998
Last modified: December 9, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.