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Protein

tRNA-splicing endonuclease

Gene

endA

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei246UniRule annotation1
Active sitei257UniRule annotation1
Active sitei287UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processtRNA processing

Enzyme and pathway databases

BRENDAi4.6.1.16 414

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonucleaseUniRule annotation (EC:4.6.1.16UniRule annotation)
Alternative name(s):
tRNA-intron endonucleaseUniRule annotation
Gene namesi
Name:endAUniRule annotation
Ordered Locus Names:AF_0900
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001094841 – 305tRNA-splicing endonucleaseAdd BLAST305

Proteomic databases

PRIDEiO29362

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi224325.AF0900

Structurei

Secondary structure

1305
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 10Combined sources7
Helixi13 – 18Combined sources6
Beta strandi21 – 24Combined sources4
Beta strandi27 – 30Combined sources4
Helixi32 – 40Combined sources9
Helixi49 – 59Combined sources11
Beta strandi60 – 62Combined sources3
Helixi63 – 75Combined sources13
Beta strandi81 – 83Combined sources3
Beta strandi86 – 97Combined sources12
Helixi104 – 110Combined sources7
Turni111 – 113Combined sources3
Beta strandi118 – 122Combined sources5
Beta strandi128 – 134Combined sources7
Beta strandi149 – 154Combined sources6
Beta strandi157 – 161Combined sources5
Helixi164 – 167Combined sources4
Beta strandi172 – 175Combined sources4
Beta strandi178 – 181Combined sources4
Helixi183 – 191Combined sources9
Beta strandi195 – 197Combined sources3
Helixi202 – 212Combined sources11
Helixi216 – 228Combined sources13
Beta strandi232 – 235Combined sources4
Helixi237 – 239Combined sources3
Beta strandi241 – 247Combined sources7
Helixi252 – 257Combined sources6
Beta strandi259 – 264Combined sources6
Helixi272 – 284Combined sources13
Beta strandi288 – 293Combined sources6
Beta strandi296 – 303Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R0VX-ray2.00A/B/C/D1-305[»]
1R11X-ray2.70A/B1-305[»]
1RLVX-ray3.00A/B1-305[»]
2GJWX-ray2.85A/B/C/D2-305[»]
3P1YX-ray2.05A/B/C/D1-305[»]
ProteinModelPortaliO29362
SMRiO29362
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO29362

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01701 Archaea
COG1676 LUCA
KOiK01170
OMAiGADFRTY
OrthoDBiPOG093Z0DMC

Family and domain databases

Gene3Di3.40.1350.10, 1 hit
HAMAPiMF_01834 EndA_long, 1 hit
InterProiView protein in InterPro
IPR011856 tRNA_endonuc-like_dom_sf
IPR036167 tRNA_intron_Endo_cat-like_sf
IPR006677 tRNA_intron_Endonuc_cat-like
IPR006678 tRNA_intron_Endonuc_N
IPR036740 tRNA_intron_Endonuc_N_sf
IPR006676 tRNA_splic
IPR023516 tRNA_splic_arch_long
PfamiView protein in Pfam
PF01974 tRNA_int_endo, 1 hit
PF02778 tRNA_int_endo_N, 1 hit
SUPFAMiSSF53032 SSF53032, 2 hits
SSF55267 SSF55267, 2 hits
TIGRFAMsiTIGR00324 endA, 1 hit

Sequencei

Sequence statusi: Complete.

O29362-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIGGDFAVVK AKKSLERRGF GVKRGDKIYL HPLEVVYLQI KGIESFGELE
60 70 80 90 100
DVLSWAESRM EDFSTYYFVY EDLRDRGNKV KIQGEFLLTK KPYLPISERK
110 120 130 140 150
TIRMEEIAEK ARNFDELRLA VVDEESEITY FRVYEPDMMG EQKEELPEIA
160 170 180 190 200
GILSDEYVIT KQTEIFSRYF YGSEKGDLVT LSLIESLYLL DLGKLNLLNA
210 220 230 240 250
DREELVKRAR EVERNFDRRY EVYRNLKERG FVVKTGFKFG SEFRVYRKVE
260 270 280 290 300
SVDDLPHSEY LVDIADSREI RLIDLARAVR LAQNVRKRMV FAYGKNYLCF

ERVKV
Length:305
Mass (Da):35,959
Last modified:January 1, 1998 - v1
Checksum:iDC0B5A5DEBD99E35
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA Translation: AAB90338.1
PIRiD69362

Genome annotation databases

EnsemblBacteriaiAAB90338; AAB90338; AF_0900
KEGGiafu:AF_0900

Similar proteinsi

Entry informationi

Entry nameiENDA_ARCFU
AccessioniPrimary (citable) accession number: O29362
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health