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O29362 (ENDA_ARCFU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA-splicing endonuclease

EC=4.6.1.16
Alternative name(s):
tRNA-intron endonuclease
Gene names
Name:endA
Ordered Locus Names:AF_0900
OrganismArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) [Reference proteome] [HAMAP]
Taxonomic identifier224325 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus

Protein attributes

Sequence length305 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp. HAMAP-Rule MF_01834

Catalytic activity

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus. HAMAP-Rule MF_01834

Subunit structure

Homodimer.

Sequence similarities

Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.

Ontologies

Keywords
   Biological processtRNA processing
   Molecular functionLyase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtRNA splicing, via endonucleolytic cleavage and ligation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionnucleic acid binding

Inferred from electronic annotation. Source: InterPro

tRNA-intron endonuclease activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 305305tRNA-splicing endonuclease HAMAP-Rule MF_01834
PRO_0000109484

Sites

Active site2461 By similarity
Active site2571 By similarity
Active site2871 By similarity

Secondary structure

............................................................ 305
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O29362 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: DC0B5A5DEBD99E35

FASTA30535,959
        10         20         30         40         50         60 
MIGGDFAVVK AKKSLERRGF GVKRGDKIYL HPLEVVYLQI KGIESFGELE DVLSWAESRM 

        70         80         90        100        110        120 
EDFSTYYFVY EDLRDRGNKV KIQGEFLLTK KPYLPISERK TIRMEEIAEK ARNFDELRLA 

       130        140        150        160        170        180 
VVDEESEITY FRVYEPDMMG EQKEELPEIA GILSDEYVIT KQTEIFSRYF YGSEKGDLVT 

       190        200        210        220        230        240 
LSLIESLYLL DLGKLNLLNA DREELVKRAR EVERNFDRRY EVYRNLKERG FVVKTGFKFG 

       250        260        270        280        290        300 
SEFRVYRKVE SVDDLPHSEY LVDIADSREI RLIDLARAVR LAQNVRKRMV FAYGKNYLCF 


ERVKV 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000782 Genomic DNA. Translation: AAB90338.1.
PIRD69362.
RefSeqNP_069733.1. NC_000917.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1R0VX-ray2.00A/B/C/D1-305[»]
1R11X-ray2.70A/B1-305[»]
1RLVX-ray3.00A/B1-305[»]
2GJWX-ray2.85A/B/C/D2-305[»]
3P1YX-ray2.05A/B/C/D1-305[»]
ProteinModelPortalO29362.
SMRO29362. Positions 2-305.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224325.AF0900.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB90338; AAB90338; AF_0900.
GeneID1484123.
KEGGafu:AF0900.

Phylogenomic databases

eggNOGCOG1676.
KOK01170.
OMARGFVVKT.
ProtClustDBPRK09300.

Enzyme and pathway databases

BioCycAFUL224325:GJBC-922-MONOMER.

Family and domain databases

Gene3D3.40.1350.10. 2 hits.
HAMAPMF_01834. EndA_long.
InterProIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR023516. tRNA_splic_arch_long.
[Graphical view]
PfamPF01974. tRNA_int_endo. 1 hit.
PF02778. tRNA_int_endo_N. 1 hit.
[Graphical view]
SUPFAMSSF53032. SSF53032. 2 hits.
SSF55267. SSF55267. 2 hits.
TIGRFAMsTIGR00324. endA. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceO29362.

Entry information

Entry nameENDA_ARCFU
AccessionPrimary (citable) accession number: O29362
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: April 16, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references