O29342 (SYD_ARCFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--tRNA ligase
EC=6.1.1.12

Alternative name(s):
Aspartyl-tRNA synthetase
Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	AF_0920

Organism

Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)

Taxonomic identifier

224325 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	430 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044_A
Cofactor	Binds 3 magnesium ions per subunit By similarity. HAMAP MF_00044_A
Subcellular location	Cytoplasm HAMAP MF_00044_A.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aspartate-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 430	430	Aspartate--tRNA ligase HAMAP MF_00044_A		PRO_0000110990
Sites
	Metal binding	353	1	Magnesium 2 By similarity
	Metal binding	353	1	Magnesium 3 By similarity
	Metal binding	356	1	Magnesium 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	O29342 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: ECC9AFE7564D65C8	FASTA	430	49,499
	10 20 30 40 50 60 MRVYTADVKP EMEGQKVTLY GWVHEVRDLG GLVFILLRDR EGIVQITLPR KFVSKQVFKL 70 80 90 100 110 120 AKKIRRESVI AVTGEVRREE KAPGGVEIIP ESIEVLNEAD APLPLEVTEK VPAELDTRLD 130 140 150 160 170 180 HRFMDLRRPR VQAIFRIRHQ VMQSVREFLS EEGFIEVHTP KIVSTATEGG TELFPISYFE 190 200 210 220 230 240 KEAFLNQSPQ LYKQVLMAAG FEKVFEIGPI FRAEEHNTTR HLNEAISIDI EMSFTDHNGV 250 260 270 280 290 300 MDVLERLVQR VYEDVAEKCE RYLGWLEVSL EIPELPFPRI TYDEAREIAA RKGEEIPWGE 310 320 330 340 350 360 DLSTNALKLV GEEMGGLYFI TDWPTESKPF YAMPYEDRPE ISKSFDLMHG WLELSSGAQR 370 380 390 400 410 420 IHLYDMLVES IKAKGMEPES FGFYLEAFRY GMPPHAGWGL GAERLIMSML GLKNVREAVL 430 FPRDRHRLVP

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000782 Genomic DNA. Translation: AAB90318.1.
PIR	H69364.
RefSeq	NP_069753.1. NC_000917.1.
3D structure databases
ProteinModelPortal	O29342.
SMR	O29342. Positions 4-430.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1484143.
GenomeReviews	Gene locus AF_0920 in contig AE000782_GR.
KEGG	afu:AF0920.
NMPDR	fig|224325.1.peg.912.
TIGR	AF_0920.
Phylogenomic databases
HOGENOM	HBG745843.
OMA	VAPKQKV.
PhylomeDB	O29342.
ProtClustDB	PRK05159.
Enzyme and pathway databases
BioCyc	AFUL224325:AF_0920-MONOMER.
Family and domain databases
HAMAP	MF_00044_A. Asp_tRNA_synth_A. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR020780. Asp-tRNA-synth_IIb_arc. IPR004523. Asp-tRNA-synth_IIb_arc/euk. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01876.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF10. PTHR22594:SF10. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00458. AspS_arch. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_ARCFU

Accession

Primary (citable) accession number: O29342

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	January 25, 2012
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program