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Protein

Glutamine synthetase

Gene

glnA

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Probably involved in nitrogen metabolism via ammonium assimilation. Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia.By similarity

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.By similarity

Cofactori

Mg2+By similarityNote: Binds 2 Mg2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi143Magnesium 1By similarity1
Metal bindingi145Magnesium 2By similarity1
Binding sitei225ATPBy similarity1
Metal bindingi230Magnesium 2By similarity1
Metal bindingi238Magnesium 2By similarity1
Binding sitei283L-glutamate; via carbonyl oxygenBy similarity1
Metal bindingi287Magnesium 1; via pros nitrogenBy similarity1
Binding sitei291ATPBy similarity1
Binding sitei344L-glutamateBy similarity1
Binding sitei350L-glutamateBy similarity1
Binding sitei362ATPBy similarity1
Binding sitei362L-glutamateBy similarity1
Binding sitei367ATPBy similarity1
Metal bindingi381Magnesium 1By similarity1
Binding sitei383L-glutamateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi289 – 291ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.1.2. 414.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetaseBy similarity (EC:6.3.1.2By similarity)
Short name:
GSBy similarity
Alternative name(s):
Glutamate--ammonia ligaseBy similarity
Glutamine synthetase I alphaBy similarity
Short name:
GSI alphaBy similarity
Gene namesi
Name:glnABy similarity
Ordered Locus Names:AF_0949
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001532011 – 491Glutamine synthetaseAdd BLAST491

Interactioni

Subunit structurei

Oligomer of 12 subunits arranged in the form of two hexagons.By similarity

Protein-protein interaction databases

STRINGi224325.AF0949.

Structurei

3D structure databases

ProteinModelPortaliO29313.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni282 – 283L-glutamate bindingBy similarity2

Sequence similaritiesi

Belongs to the glutamine synthetase family.By similarity

Phylogenomic databases

eggNOGiarCOG01909. Archaea.
COG0174. LUCA.
KOiK01915.
OMAiDTWIWYK.
OrthoDBiPOG093Z00GE.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiView protein in PROSITE
PS00181. GLNA_ATP. 1 hit.

Sequencei

Sequence statusi: Complete.

O29313-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRRLRGDCM EEVERAKAVL KENNVRQVLC AFADVRGYLQ MFSIPAREFV
60 70 80 90 100
DGSAFENGIG FDGSSVRGFR TIEKSDMVWM PDASSLKIIP WIDDPIQKSA
110 120 130 140 150
IMFGNVYEAW GTEIADCDPR GYVAKRYEDM LKSEGMSAIF GPEIEFFLFE
160 170 180 190 200
GVDFTRLSWD MWVSPNGGAG DSWGPPRIMP ISSELESGYM IRPKEGYFRP
210 220 230 240 250
PPEDTTVEYR NELVYYLEQL GIDIEYHHHE VATAGQVELD FKPKQLVDVG
260 270 280 290 300
DAFYLYKFAA KNIAAMHGLY ATFMPKPLYL DNASGMHTHQ SLWKGEPFSG
310 320 330 340 350
EAVFADPDDE YMLSQKARYY IGGLLEHAKA LTALCAPTVN SYKRLVPGFE
360 370 380 390 400
APIYICWSPR NRSALVRVPM YVKKPSAIRV EYRGVDPSCN PYLAITAQLA
410 420 430 440 450
AGLDGIKKKI DPGDPLLEDV YELTPAQKRE LGVGELPTTL RDAIDHLASD
460 470 480 490
ELMQEVLGSH IFDAFMELKI DEWNQYCLYI TPWEFMKYFD I
Length:491
Mass (Da):55,723
Last modified:January 1, 1998 - v1
Checksum:i5AADF719AF23F25D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB90294.1.
PIRiE69368.
RefSeqiWP_010878449.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB90294; AAB90294; AF_0949.
GeneIDi1484172.
KEGGiafu:AF_0949.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB90294.1.
PIRiE69368.
RefSeqiWP_010878449.1. NC_000917.1.

3D structure databases

ProteinModelPortaliO29313.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224325.AF0949.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB90294; AAB90294; AF_0949.
GeneIDi1484172.
KEGGiafu:AF_0949.

Phylogenomic databases

eggNOGiarCOG01909. Archaea.
COG0174. LUCA.
KOiK01915.
OMAiDTWIWYK.
OrthoDBiPOG093Z00GE.

Enzyme and pathway databases

BRENDAi6.3.1.2. 414.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiView protein in PROSITE
PS00181. GLNA_ATP. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLN1A_ARCFU
AccessioniPrimary (citable) accession number: O29313
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: March 15, 2017
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.