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Protein

Glutamine synthetase

Gene

glnA

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probably involved in nitrogen metabolism via ammonium assimilation. Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia. Beta-glutamate is a much poorer substrate than alpha-glutamate.1 Publication

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.1 Publication

Cofactori

Mg2+By similarityNote: Binds 2 Mg2+ ions per subunit.By similarity

Kineticsi

  1. KM=0.56 mM for ATP1 Publication
  2. KM=3 mM for alpha-glutamate1 Publication

    Temperature dependencei

    Optimum temperature is 83 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi143Magnesium 1By similarity1
    Metal bindingi145Magnesium 2By similarity1
    Binding sitei225ATPBy similarity1
    Metal bindingi230Magnesium 2By similarity1
    Metal bindingi238Magnesium 2By similarity1
    Binding sitei283L-glutamate; via carbonyl oxygenBy similarity1
    Metal bindingi287Magnesium 1; via pros nitrogenBy similarity1
    Binding sitei291ATPBy similarity1
    Binding sitei344L-glutamateBy similarity1
    Binding sitei350L-glutamateBy similarity1
    Binding sitei362ATPBy similarity1
    Binding sitei362L-glutamateBy similarity1
    Binding sitei367ATPBy similarity1
    Metal bindingi381Magnesium 1By similarity1
    Binding sitei383L-glutamateBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi289 – 291ATPBy similarity3

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionLigase
    LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi6.3.1.2. 414.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine synthetase1 Publication (EC:6.3.1.21 Publication)
    Short name:
    GS1 Publication
    Alternative name(s):
    Glutamate--ammonia ligaseBy similarity
    Glutamine synthetase I alpha1 Publication
    Short name:
    GSI alpha1 Publication
    Gene namesi
    Name:glnABy similarity
    Ordered Locus Names:AF_0949
    OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
    Taxonomic identifieri224325 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
    Proteomesi
    • UP000002199 Componenti: Chromosome

    Subcellular locationi

    • Cytoplasm By similarity

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001532011 – 491Glutamine synthetaseAdd BLAST491

    Interactioni

    Subunit structurei

    Oligomer of 12 subunits arranged in the form of two hexagons.By similarity

    Protein-protein interaction databases

    STRINGi224325.AF0949.

    Structurei

    3D structure databases

    ProteinModelPortaliO29313.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni282 – 283L-glutamate bindingBy similarity2

    Sequence similaritiesi

    Belongs to the glutamine synthetase family.By similarity

    Phylogenomic databases

    eggNOGiarCOG01909. Archaea.
    COG0174. LUCA.
    KOiK01915.
    OMAiDTWIWYK.
    OrthoDBiPOG093Z00GE.

    Family and domain databases

    Gene3Di3.10.20.70. 1 hit.
    3.30.590.10. 1 hit.
    InterProiView protein in InterPro
    IPR008147. Gln_synt_b-grasp.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR004809. Gln_synth_I.
    PfamiView protein in Pfam
    PF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    SMARTiView protein in SMART
    SM01230. Gln-synt_C. 1 hit.
    SUPFAMiSSF54368. SSF54368. 1 hit.
    TIGRFAMsiTIGR00653. GlnA. 1 hit.
    PROSITEiView protein in PROSITE
    PS00181. GLNA_ATP. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O29313-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MVRRLRGDCM EEVERAKAVL KENNVRQVLC AFADVRGYLQ MFSIPAREFV
    60 70 80 90 100
    DGSAFENGIG FDGSSVRGFR TIEKSDMVWM PDASSLKIIP WIDDPIQKSA
    110 120 130 140 150
    IMFGNVYEAW GTEIADCDPR GYVAKRYEDM LKSEGMSAIF GPEIEFFLFE
    160 170 180 190 200
    GVDFTRLSWD MWVSPNGGAG DSWGPPRIMP ISSELESGYM IRPKEGYFRP
    210 220 230 240 250
    PPEDTTVEYR NELVYYLEQL GIDIEYHHHE VATAGQVELD FKPKQLVDVG
    260 270 280 290 300
    DAFYLYKFAA KNIAAMHGLY ATFMPKPLYL DNASGMHTHQ SLWKGEPFSG
    310 320 330 340 350
    EAVFADPDDE YMLSQKARYY IGGLLEHAKA LTALCAPTVN SYKRLVPGFE
    360 370 380 390 400
    APIYICWSPR NRSALVRVPM YVKKPSAIRV EYRGVDPSCN PYLAITAQLA
    410 420 430 440 450
    AGLDGIKKKI DPGDPLLEDV YELTPAQKRE LGVGELPTTL RDAIDHLASD
    460 470 480 490
    ELMQEVLGSH IFDAFMELKI DEWNQYCLYI TPWEFMKYFD I
    Length:491
    Mass (Da):55,723
    Last modified:January 1, 1998 - v1
    Checksum:i5AADF719AF23F25D
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE000782 Genomic DNA. Translation: AAB90294.1.
    PIRiE69368.
    RefSeqiWP_010878449.1. NC_000917.1.

    Genome annotation databases

    EnsemblBacteriaiAAB90294; AAB90294; AF_0949.
    GeneIDi1484172.
    KEGGiafu:AF_0949.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE000782 Genomic DNA. Translation: AAB90294.1.
    PIRiE69368.
    RefSeqiWP_010878449.1. NC_000917.1.

    3D structure databases

    ProteinModelPortaliO29313.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi224325.AF0949.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAB90294; AAB90294; AF_0949.
    GeneIDi1484172.
    KEGGiafu:AF_0949.

    Phylogenomic databases

    eggNOGiarCOG01909. Archaea.
    COG0174. LUCA.
    KOiK01915.
    OMAiDTWIWYK.
    OrthoDBiPOG093Z00GE.

    Enzyme and pathway databases

    BRENDAi6.3.1.2. 414.

    Family and domain databases

    Gene3Di3.10.20.70. 1 hit.
    3.30.590.10. 1 hit.
    InterProiView protein in InterPro
    IPR008147. Gln_synt_b-grasp.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR004809. Gln_synth_I.
    PfamiView protein in Pfam
    PF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    SMARTiView protein in SMART
    SM01230. Gln-synt_C. 1 hit.
    SUPFAMiSSF54368. SSF54368. 1 hit.
    TIGRFAMsiTIGR00653. GlnA. 1 hit.
    PROSITEiView protein in PROSITE
    PS00181. GLNA_ATP. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiGLN1A_ARCFU
    AccessioniPrimary (citable) accession number: O29313
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: January 1, 1998
    Last modified: April 12, 2017
    This is version 103 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.