Reviewed, UniProtKB/Swiss-Prot O29248 (ILVD_ARCFU)

Names and origin

Protein names

Recommended name:
    Dihydroxy-acid dehydratase
      Short name=DAD
    EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	AF_1014

Organism

Archaeoglobus fulgidus [Complete proteome] [HAMAP]

Taxonomic identifier

2234 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	547 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the ilvD/edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 547	547	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_0000103537
Sites
	Metal binding	119	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	191	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	O29248-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 736A640D09A8C535	FASTA	547	58,742
	10 20 30 40 50 60 MRSDEVKKGI DRVAHRALLK ALGVTDDEMD KPFIGVANAY NTIVPGHMTL DKLTQAVKEG 70 80 90 100 110 120 VYAAGGVPFE FGIIGICDGI AMGHEGMCFS LPSRELVADT IEAMVEAHRF DGLVVVASCD 130 140 150 160 170 180 KIIPGMLMAM LRLNIPAIAV TGGPMPYERV GGEKVSIKDA FEAAGMYKAG KLDDAGLKLY 190 200 210 220 230 240 EDYCAPYCGS CQGLYTANSM QILTETLGLS LPYCSTSPCP SSRKLRIAKQ SGKRVVELVM 250 260 270 280 290 300 QNIKPLDFVN ERSFENAITM DMLVGGSTNT VLHLPAIAKE AGIRLSLDLF DEISRRTPHI 310 320 330 340 350 360 VSIDPASKEM VVDLDESGGV PMLIKKARKY FHDEMTVSGK TLYEIAEMAV LRGRDIIASP 370 380 390 400 410 420 DNPLHKEGGI AILKGNLAEN GAVIKAAAVS EDMMRFEGTA KVYDSEKEAL NAILDGKVEE 430 440 450 460 470 480 GDVVVIRYMG PKGAPGMPEM LLPTAAISGL GLQKVALITD GRFSGATRGP CIGHVSPEAA 490 500 510 520 530 540 VGGNIALVED GDKISIDIPA RKLEVKLSDE ELAERRAKWK PKEKELKGYL AKYAKLVRGA EEGAALL

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000782 Genomic DNA. Translation: AAB90229.1.
PIR	F69376.
RefSeq	NP_069847.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1484237.
GenomeReviews	Gene locus AF_1014 in contig AE000782_GR.
KEGG	afu:AF1014.
NMPDR	fig|224325.1.peg.1006.
TIGR	AF_1014.
Phylogenomic databases
HOGENOM	O29248.
OMA	QGLYTAN.
Enzyme and pathway databases
BioCyc	AFUL224325:AF_1014-MON.
BRENDA	4.2.1.9. 7576.
Family and domain databases
HAMAP	MF_00012. [Tree]
InterPro	IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
ProDom	PD002691. ILVD_EDD_family. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00110. ilvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_ARCFU

Accession

Primary (citable) accession number: O29248

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	November 3, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)