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O29206

- TRMY_ARCFU

UniProt

O29206 - TRMY_ARCFU

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Protein

tRNA (pseudouridine(54)-N(1))-methyltransferase

Gene

trmY

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Specifically catalyzes the N1-methylation of pseudouridine at position 54 (Psi54) in tRNAs.By similarity

Catalytic activityi

S-adenosyl-L-methionine + pseudouridine(54) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylpseudouridine(54) in tRNA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei242 – 2421S-adenosyl-L-methionine; via amide nitrogen1 Publication
Binding sitei275 – 2751S-adenosyl-L-methionine1 Publication

GO - Molecular functioni

  1. S-adenosylmethionine-dependent methyltransferase activity Source: UniProtKB-HAMAP
  2. tRNA methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-1078-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (pseudouridine(54)-N(1))-methyltransferase (EC:2.1.1.257)
Gene namesi
Name:trmY
Ordered Locus Names:AF_1056
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
ProteomesiUP000002199: Chromosome

Subcellular locationi

Cytoplasm Curated

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 288288tRNA (pseudouridine(54)-N(1))-methyltransferasePRO_0000157945Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi224325.AF1056.

Structurei

Secondary structure

1
288
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi96 – 1049Combined sources
Turni115 – 1195Combined sources
Helixi121 – 13212Combined sources
Beta strandi142 – 1487Combined sources
Beta strandi151 – 1533Combined sources
Beta strandi156 – 1616Combined sources
Turni162 – 1643Combined sources
Helixi172 – 18413Combined sources
Beta strandi192 – 1954Combined sources
Beta strandi198 – 2014Combined sources
Helixi205 – 21511Combined sources
Beta strandi216 – 2216Combined sources
Beta strandi225 – 2273Combined sources
Helixi228 – 2303Combined sources
Beta strandi235 – 2428Combined sources
Helixi249 – 2568Combined sources
Beta strandi260 – 2645Combined sources
Helixi272 – 28716Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2QMMX-ray1.85A/B95-288[»]
ProteinModelPortaliO29206.
SMRiO29206. Positions 95-288.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO29206.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 9494UnknownAdd
BLAST
Regioni95 – 288194MethyltransferaseAdd
BLAST
Regioni221 – 2233S-adenosyl-L-methionine binding
Regioni265 – 2706S-adenosyl-L-methionine binding

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1901.
KOiK16317.
OMAiLGDHTGM.

Family and domain databases

Gene3Di3.40.1280.10. 1 hit.
HAMAPiMF_00587. tRNA_methyltr_TrmY.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR007158. TrmY.
IPR029026. tRNA_m1G_MTases_N.
[Graphical view]
PfamiPF04013. Methyltrn_RNA_2. 1 hit.
[Graphical view]
SUPFAMiSSF75217. SSF75217. 1 hit.

Sequencei

Sequence statusi: Complete.

O29206-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MENPDSAFLL FRNAPRLPLA TMHTPLCGDS HQPDFAFRKP ARDALLRPWN
60 70 80 90 100
GRNHRCGLQD GREICQGQLP AHQKSYWSDC AALPRLFRAY GGAVVRGFLI
110 120 130 140 150
VGNKAFTQPF SLNDLPGAGR MDVLCRCTSQ ALFISHGIRR DVEVYLLLLG
160 170 180 190 200
PPSPPKSILI KGDEVRRMSP DERNVAGHIK KALAVECGKS WKKVHSGVYV
210 220 230 240 250
SRKGLEELIE ELSEKYSIIY LKEDGVDISN AQLPPNPLFV IGDHEGLTEE
260 270 280
QEKVVERYAA LKLSLSPLSL LAEQCVVIAH HHLDRLQF
Length:288
Mass (Da):32,192
Last modified:January 1, 1998 - v1
Checksum:i02CD364BC26A45A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB90184.1.
PIRiH69381.
RefSeqiNP_069889.1. NC_000917.1.
WP_010878556.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB90184; AAB90184; AF_1056.
GeneIDi1484279.
KEGGiafu:AF1056.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB90184.1 .
PIRi H69381.
RefSeqi NP_069889.1. NC_000917.1.
WP_010878556.1. NC_000917.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2QMM X-ray 1.85 A/B 95-288 [» ]
ProteinModelPortali O29206.
SMRi O29206. Positions 95-288.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224325.AF1056.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB90184 ; AAB90184 ; AF_1056 .
GeneIDi 1484279.
KEGGi afu:AF1056.

Phylogenomic databases

eggNOGi COG1901.
KOi K16317.
OMAi LGDHTGM.

Enzyme and pathway databases

BioCyci AFUL224325:GJBC-1078-MONOMER.

Miscellaneous databases

EvolutionaryTracei O29206.

Family and domain databases

Gene3Di 3.40.1280.10. 1 hit.
HAMAPi MF_00587. tRNA_methyltr_TrmY.
InterProi IPR029028. Alpha/beta_knot_MTases.
IPR007158. TrmY.
IPR029026. tRNA_m1G_MTases_N.
[Graphical view ]
Pfami PF04013. Methyltrn_RNA_2. 1 hit.
[Graphical view ]
SUPFAMi SSF75217. SSF75217. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus."
    Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G.
    , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C.
    Nature 390:364-370(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.
  2. "Crystal structure of APC86534.1."
    Midwest center for structural genomics (MCSG)
    Submitted (FEB-2009) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) OF 95-288 IN COMPLEX WITH S-ADENOSYL-L-METHIONINE.

Entry informationi

Entry nameiTRMY_ARCFU
AccessioniPrimary (citable) accession number: O29206
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2002
Last sequence update: January 1, 1998
Last modified: November 26, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3