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O29206

- TRMY_ARCFU

UniProt

O29206 - TRMY_ARCFU

Protein

tRNA (pseudouridine(54)-N(1))-methyltransferase

Gene

trmY

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Specifically catalyzes the N1-methylation of pseudouridine at position 54 (Psi54) in tRNAs.By similarity

    Catalytic activityi

    S-adenosyl-L-methionine + pseudouridine(54) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylpseudouridine(54) in tRNA.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei242 – 2421S-adenosyl-L-methionine; via amide nitrogen1 Publication
    Binding sitei275 – 2751S-adenosyl-L-methionine1 Publication

    GO - Molecular functioni

    1. S-adenosylmethionine-dependent methyltransferase activity Source: UniProtKB-HAMAP
    2. tRNA methyltransferase activity Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciAFUL224325:GJBC-1078-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (pseudouridine(54)-N(1))-methyltransferase (EC:2.1.1.257)
    Gene namesi
    Name:trmY
    Ordered Locus Names:AF_1056
    OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
    Taxonomic identifieri224325 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
    ProteomesiUP000002199: Chromosome

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 288288tRNA (pseudouridine(54)-N(1))-methyltransferasePRO_0000157945Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi224325.AF1056.

    Structurei

    Secondary structure

    1
    288
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi96 – 1049
    Turni115 – 1195
    Helixi121 – 13212
    Beta strandi142 – 1487
    Beta strandi151 – 1533
    Beta strandi156 – 1616
    Turni162 – 1643
    Helixi172 – 18413
    Beta strandi192 – 1954
    Beta strandi198 – 2014
    Helixi205 – 21511
    Beta strandi216 – 2216
    Beta strandi225 – 2273
    Helixi228 – 2303
    Beta strandi235 – 2428
    Helixi249 – 2568
    Beta strandi260 – 2645
    Helixi272 – 28716

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2QMMX-ray1.85A/B95-288[»]
    ProteinModelPortaliO29206.
    SMRiO29206. Positions 95-288.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO29206.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 9494UnknownAdd
    BLAST
    Regioni95 – 288194MethyltransferaseAdd
    BLAST
    Regioni221 – 2233S-adenosyl-L-methionine binding
    Regioni265 – 2706S-adenosyl-L-methionine binding

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1901.
    KOiK16317.
    OMAiLGDHTGM.

    Family and domain databases

    Gene3Di3.40.1280.10. 1 hit.
    HAMAPiMF_00587. tRNA_methyltr_TrmY.
    InterProiIPR029028. Alpha/beta_knot_MTases.
    IPR007158. TrmY.
    IPR029026. tRNA_m1G_MTases_N.
    [Graphical view]
    PfamiPF04013. Methyltrn_RNA_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF75217. SSF75217. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O29206-1 [UniParc]FASTAAdd to Basket

    « Hide

    MENPDSAFLL FRNAPRLPLA TMHTPLCGDS HQPDFAFRKP ARDALLRPWN    50
    GRNHRCGLQD GREICQGQLP AHQKSYWSDC AALPRLFRAY GGAVVRGFLI 100
    VGNKAFTQPF SLNDLPGAGR MDVLCRCTSQ ALFISHGIRR DVEVYLLLLG 150
    PPSPPKSILI KGDEVRRMSP DERNVAGHIK KALAVECGKS WKKVHSGVYV 200
    SRKGLEELIE ELSEKYSIIY LKEDGVDISN AQLPPNPLFV IGDHEGLTEE 250
    QEKVVERYAA LKLSLSPLSL LAEQCVVIAH HHLDRLQF 288
    Length:288
    Mass (Da):32,192
    Last modified:January 1, 1998 - v1
    Checksum:i02CD364BC26A45A8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000782 Genomic DNA. Translation: AAB90184.1.
    PIRiH69381.
    RefSeqiNP_069889.1. NC_000917.1.

    Genome annotation databases

    EnsemblBacteriaiAAB90184; AAB90184; AF_1056.
    GeneIDi1484279.
    KEGGiafu:AF1056.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000782 Genomic DNA. Translation: AAB90184.1 .
    PIRi H69381.
    RefSeqi NP_069889.1. NC_000917.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2QMM X-ray 1.85 A/B 95-288 [» ]
    ProteinModelPortali O29206.
    SMRi O29206. Positions 95-288.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224325.AF1056.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB90184 ; AAB90184 ; AF_1056 .
    GeneIDi 1484279.
    KEGGi afu:AF1056.

    Phylogenomic databases

    eggNOGi COG1901.
    KOi K16317.
    OMAi LGDHTGM.

    Enzyme and pathway databases

    BioCyci AFUL224325:GJBC-1078-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei O29206.

    Family and domain databases

    Gene3Di 3.40.1280.10. 1 hit.
    HAMAPi MF_00587. tRNA_methyltr_TrmY.
    InterProi IPR029028. Alpha/beta_knot_MTases.
    IPR007158. TrmY.
    IPR029026. tRNA_m1G_MTases_N.
    [Graphical view ]
    Pfami PF04013. Methyltrn_RNA_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF75217. SSF75217. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus."
      Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G.
      , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C.
      Nature 390:364-370(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.
    2. "Crystal structure of APC86534.1."
      Midwest center for structural genomics (MCSG)
      Submitted (FEB-2009) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) OF 95-288 IN COMPLEX WITH S-ADENOSYL-L-METHIONINE.

    Entry informationi

    Entry nameiTRMY_ARCFU
    AccessioniPrimary (citable) accession number: O29206
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 25, 2002
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3