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Protein

tRNA (pseudouridine(54)-N(1))-methyltransferase

Gene

trmY

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Specifically catalyzes the N1-methylation of pseudouridine at position 54 (Psi54) in tRNAs.By similarity

Catalytic activityi

S-adenosyl-L-methionine + pseudouridine(54) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylpseudouridine(54) in tRNA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei242S-adenosyl-L-methionine; via amide nitrogen1 Publication1
Binding sitei275S-adenosyl-L-methionine1 Publication1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (pseudouridine(54)-N(1))-methyltransferase (EC:2.1.1.257)
Gene namesi
Name:trmY
Ordered Locus Names:AF_1056
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001579451 – 288tRNA (pseudouridine(54)-N(1))-methyltransferaseAdd BLAST288

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi224325.AF1056.

Structurei

Secondary structure

1288
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi96 – 104Combined sources9
Turni115 – 119Combined sources5
Helixi121 – 132Combined sources12
Beta strandi142 – 148Combined sources7
Beta strandi151 – 153Combined sources3
Beta strandi156 – 161Combined sources6
Turni162 – 164Combined sources3
Helixi172 – 184Combined sources13
Beta strandi192 – 195Combined sources4
Beta strandi198 – 201Combined sources4
Helixi205 – 215Combined sources11
Beta strandi216 – 221Combined sources6
Beta strandi225 – 227Combined sources3
Helixi228 – 230Combined sources3
Beta strandi235 – 242Combined sources8
Helixi249 – 256Combined sources8
Beta strandi260 – 264Combined sources5
Helixi272 – 287Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2QMMX-ray1.85A/B95-288[»]
ProteinModelPortaliO29206.
SMRiO29206.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO29206.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 94UnknownAdd BLAST94
Regioni95 – 288MethyltransferaseAdd BLAST194
Regioni221 – 223S-adenosyl-L-methionine binding3
Regioni265 – 270S-adenosyl-L-methionine binding6

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG01239. Archaea.
COG1901. LUCA.
KOiK16317.
OMAiLGDHTGM.

Family and domain databases

Gene3Di3.40.1280.10. 1 hit.
HAMAPiMF_00587. tRNA_methyltr_TrmY. 1 hit.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR007158. TrmY.
IPR029026. tRNA_m1G_MTases_N.
[Graphical view]
PfamiPF04013. Methyltrn_RNA_2. 1 hit.
[Graphical view]
SUPFAMiSSF75217. SSF75217. 1 hit.

Sequencei

Sequence statusi: Complete.

O29206-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENPDSAFLL FRNAPRLPLA TMHTPLCGDS HQPDFAFRKP ARDALLRPWN
60 70 80 90 100
GRNHRCGLQD GREICQGQLP AHQKSYWSDC AALPRLFRAY GGAVVRGFLI
110 120 130 140 150
VGNKAFTQPF SLNDLPGAGR MDVLCRCTSQ ALFISHGIRR DVEVYLLLLG
160 170 180 190 200
PPSPPKSILI KGDEVRRMSP DERNVAGHIK KALAVECGKS WKKVHSGVYV
210 220 230 240 250
SRKGLEELIE ELSEKYSIIY LKEDGVDISN AQLPPNPLFV IGDHEGLTEE
260 270 280
QEKVVERYAA LKLSLSPLSL LAEQCVVIAH HHLDRLQF
Length:288
Mass (Da):32,192
Last modified:January 1, 1998 - v1
Checksum:i02CD364BC26A45A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB90184.1.
PIRiH69381.

Genome annotation databases

EnsemblBacteriaiAAB90184; AAB90184; AF_1056.
KEGGiafu:AF_1056.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB90184.1.
PIRiH69381.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2QMMX-ray1.85A/B95-288[»]
ProteinModelPortaliO29206.
SMRiO29206.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224325.AF1056.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB90184; AAB90184; AF_1056.
KEGGiafu:AF_1056.

Phylogenomic databases

eggNOGiarCOG01239. Archaea.
COG1901. LUCA.
KOiK16317.
OMAiLGDHTGM.

Miscellaneous databases

EvolutionaryTraceiO29206.

Family and domain databases

Gene3Di3.40.1280.10. 1 hit.
HAMAPiMF_00587. tRNA_methyltr_TrmY. 1 hit.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR007158. TrmY.
IPR029026. tRNA_m1G_MTases_N.
[Graphical view]
PfamiPF04013. Methyltrn_RNA_2. 1 hit.
[Graphical view]
SUPFAMiSSF75217. SSF75217. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRMY_ARCFU
AccessioniPrimary (citable) accession number: O29206
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2002
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.