O29196 (MER_ARCFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5,10-methylenetetrahydromethanopterin reductase
EC=1.5.99.11

Alternative name(s):
Coenzyme F420-dependent N(5),N(10)-methylenetetrahydromethanopterin reductase
Methylene-H(4)MPT reductase

Gene names

Name:	mer
Ordered Locus Names:	AF_1066

Organism

Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)

Taxonomic identifier

224325 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidation of methyl-H4MPT to methylene-H4MPT By similarity. HAMAP MF_01091
Catalytic activity	5-methyltetrahydromethanopterin + coenzyme F420 = 5,10-methylenetetrahydromethanopterin + reduced coenzyme F420. HAMAP MF_01091
Pathway	Metabolic intermediate metabolism; lactate oxidation. HAMAP MF_01091
Subcellular location	Cytoplasm By similarity HAMAP MF_01091.
Sequence similarities	Belongs to the mer family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	coenzyme F420-dependent N5,N10-methenyltetrahydromethanopterin reductase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 332	332	5,10-methylenetetrahydromethanopterin reductase HAMAP MF_01091		PRO_0000084805

Sequences

	Sequence		Length	Mass (Da)
	O29196 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: EA4264DF2727E4D9	FASTA	332	36,051
	10 20 30 40 50 60 MKFGIEFVPD MKYYELEYYV KLAEDSGFDY TWITDHYNNR NVYSMLTILA LKTRTIKLGP 70 80 90 100 110 120 GVTNPYHISP ALTASAIGTI NEISGGRAVL GIGAGDKVTF ERIGITWEKP LKRMREAVEI 130 140 150 160 170 180 IRQLTEGKAV KYDGEIFKFN GAKLGFKPGS IPIYIGAQGP KMLQLAAELG DGVLINASHP 190 200 210 220 230 240 KDFEVAKENI DAGLAKAGKS RDAFDTVAYA SMSVDKDRDK ARNAARIVVA FIVAGSPPTV 250 260 270 280 290 300 LERHGLSEDA VNAVREALNN AFTKGDWGGV AKSVTDEMID IFSISGTPDD VIERINELSK 310 320 330 AGVTQVVAGS PIGPDKKKSI QLIGKEIIPK LK

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000782 Genomic DNA. Translation: AAB90174.1.
PIR	B69383.
RefSeq	NP_069899.1. NC_000917.1.
3D structure databases
ProteinModelPortal	O29196.
SMR	O29196. Positions 1-330.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1484290.
GenomeReviews	Gene locus AF_1066 in contig AE000782_GR.
KEGG	afu:AF1066.
NMPDR	fig|224325.1.peg.1058.
TIGR	AF_1066.
Phylogenomic databases
HOGENOM	HBG497169.
OMA	GIEFVPG.
ProtClustDB	PRK02271.
Enzyme and pathway databases
BioCyc	AFUL224325:AF_1066-MONOMER.
Family and domain databases
HAMAP	MF_01091. F420_mer. [Tree]
InterPro	IPR011251. Luciferase-like_dom. IPR019946. MeH4methanopterin_reductase. [Graphical view]
Gene3D	G3DSA:3.20.20.30. Luciferase_like. 1 hit.
KO	K00320.
Pfam	PF00296. Bac_luciferase. 1 hit. [Graphical view]
SUPFAM	SSF51679. Luciferase_like. 1 hit.
TIGRFAMs	TIGR03555. F420_mer. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MER_ARCFU

Accession

Primary (citable) accession number: O29196

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	January 1, 1998
Last modified:	November 16, 2011
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program