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Protein

tRNA N6-adenosine threonylcarbamoyltransferase

Gene

kae1

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Kae1 likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity.UniRule annotation

Catalytic activityi

L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 1 Fe2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi105 – 1051IronUniRule annotation
Metal bindingi109 – 1091IronUniRule annotation
Metal bindingi126 – 1261IronUniRule annotation
Binding sitei158 – 1581SubstrateUniRule annotation
Binding sitei171 – 1711Substrate; via amide nitrogenUniRule annotation
Binding sitei175 – 1751SubstrateUniRule annotation
Binding sitei255 – 2551SubstrateUniRule annotation
Metal bindingi283 – 2831IronUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-1134-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.3.1.234UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthaseUniRule annotation
Short name:
t(6)A synthaseUniRule annotation
t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
Gene namesi
Name:kae1UniRule annotation
Ordered Locus Names:AF_1112
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 323323tRNA N6-adenosine threonylcarbamoyltransferasePRO_0000303631Add
BLAST

Interactioni

Subunit structurei

Monomer. Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.UniRule annotation

Protein-protein interaction databases

STRINGi224325.AF1112.

Structurei

3D structure databases

ProteinModelPortaliO29153.
SMRiO29153. Positions 1-322.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni126 – 1305Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the KAE1 / TsaD family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01183. Archaea.
COG0533. LUCA.
KOiK01409.
OMAiLMIAHAG.

Family and domain databases

HAMAPiMF_01446. Kae1. 1 hit.
InterProiIPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/TsaD.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.

Sequencei

Sequence statusi: Complete.

O29153-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIALGIEGTA WSLSIGVVDE EGVIALENDP YIPKEGGIHP REASQHHSER
60 70 80 90 100
LPSLLSRVFE KVDKNSIDVV AFSQGPGMGP CLRVVATAAR LLAIKLEKPL
110 120 130 140 150
VGVNHCLAHV EVGRWQTGAR KPVSLYVSGG NSQVIARRGN RYRVFGETLD
160 170 180 190 200
IGIGNALDKL ARHMGLKHPG GPKIEELAKK GQKYHFLPYV VKGMDFSFSG
210 220 230 240 250
MVTAAQRLFD SGVRMEDVAF SFQETAFAML TEVTERALAY LDLNEVLLVG
260 270 280 290 300
GVAANKRLQE MLRIMCEDRG AKFYVPPKEL AGDNGAMIAY TGLLMYKHGH
310 320
QTPVEKSYVR PDFRIEDVEV NWD
Length:323
Mass (Da):35,604
Last modified:January 1, 1998 - v1
Checksum:i57373492D614002D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB90129.1.
PIRiG69388.
RefSeqiWP_010878608.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB90129; AAB90129; AF_1112.
GeneIDi24794717.
KEGGiafu:AF_1112.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB90129.1.
PIRiG69388.
RefSeqiWP_010878608.1. NC_000917.1.

3D structure databases

ProteinModelPortaliO29153.
SMRiO29153. Positions 1-322.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224325.AF1112.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB90129; AAB90129; AF_1112.
GeneIDi24794717.
KEGGiafu:AF_1112.

Phylogenomic databases

eggNOGiarCOG01183. Archaea.
COG0533. LUCA.
KOiK01409.
OMAiLMIAHAG.

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-1134-MONOMER.

Family and domain databases

HAMAPiMF_01446. Kae1. 1 hit.
InterProiIPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/TsaD.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKAE1_ARCFU
AccessioniPrimary (citable) accession number: O29153
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.