O29153 (KAE1_ARCFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable tRNA threonylcarbamoyladenosine biosynthesis protein KAE1 homolog

Alternative name(s):
t(6)A37 threonylcarbamoyladenosine biosynthesis protein KAE1 homolog

Gene names

Ordered Locus Names:

AF_1112

Organism

Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)

Taxonomic identifier

224325 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine By similarity. HAMAP MF_01446
Subcellular location	Cytoplasm Potential HAMAP MF_01446.
Sequence similarities	Belongs to the KAE1 / YgjD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 323	323	Probable tRNA threonylcarbamoyladenosine biosynthesis protein KAE1 homolog HAMAP MF_01446		PRO_0000303631
Sites
	Metal binding	105	1	Iron By similarity
	Metal binding	109	1	Iron By similarity
	Metal binding	126	1	Iron By similarity
	Metal binding	283	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	O29153 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 57373492D614002D	FASTA	323	35,604
	10 20 30 40 50 60 MIALGIEGTA WSLSIGVVDE EGVIALENDP YIPKEGGIHP REASQHHSER LPSLLSRVFE 70 80 90 100 110 120 KVDKNSIDVV AFSQGPGMGP CLRVVATAAR LLAIKLEKPL VGVNHCLAHV EVGRWQTGAR 130 140 150 160 170 180 KPVSLYVSGG NSQVIARRGN RYRVFGETLD IGIGNALDKL ARHMGLKHPG GPKIEELAKK 190 200 210 220 230 240 GQKYHFLPYV VKGMDFSFSG MVTAAQRLFD SGVRMEDVAF SFQETAFAML TEVTERALAY 250 260 270 280 290 300 LDLNEVLLVG GVAANKRLQE MLRIMCEDRG AKFYVPPKEL AGDNGAMIAY TGLLMYKHGH 310 320 QTPVEKSYVR PDFRIEDVEV NWD

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000782 Genomic DNA. Translation: AAB90129.1.
PIR	G69388.
RefSeq	NP_069941.1. NC_000917.1.
3D structure databases
ProteinModelPortal	O29153.
SMR	O29153. Positions 1-322.
ModBase	Search...
Protein family/group databases
MEROPS	M22.950.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1484334.
GenomeReviews	Gene locus AF_1112 in contig AE000782_GR.
KEGG	afu:AF1112.
NMPDR	fig|224325.1.peg.1100.
TIGR	AF_1112.
Phylogenomic databases
HOGENOM	HBG304663.
OMA	RRTYITP.
PhylomeDB	O29153.
ProtClustDB	PRK14878.
Enzyme and pathway databases
BioCyc	AFUL224325:AF_1112-MONOMER.
Family and domain databases
HAMAP	MF_01446. Kae1_arch. [Tree]
InterPro	IPR022449. Kae1_pept_M22-rel. IPR000905. Peptidase_M22. IPR017861. Peptidase_M22_subgr. [Graphical view]
KO	K01409.
Pfam	PF00814. Peptidase_M22. 1 hit. [Graphical view]
PRINTS	PR00789. OSIALOPTASE.
TIGRFAMs	TIGR03722. Arch_KAE1. 1 hit. TIGR00329. Gcp_kae1. 1 hit.
PROSITE	PS01016. GLYCOPROTEASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KAE1_ARCFU

Accession

Primary (citable) accession number: O29153

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	January 1, 1998
Last modified:	December 14, 2011
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program