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Protein

tRNA N6-adenosine threonylcarbamoyltransferase

Gene

kae1

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Kae1 likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity.UniRule annotation

Catalytic activityi

L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N6-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 1 Fe2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi105IronUniRule annotation1
Metal bindingi109IronUniRule annotation1
Metal bindingi126IronUniRule annotation1
Binding sitei158SubstrateUniRule annotation1
Binding sitei171Substrate; via amide nitrogenUniRule annotation1
Binding sitei175SubstrateUniRule annotation1
Binding sitei255SubstrateUniRule annotation1
Metal bindingi283IronUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processtRNA processing
LigandIron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.3.1.234UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthaseUniRule annotation
Short name:
t(6)A synthaseUniRule annotation
t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
Gene namesi
Name:kae1UniRule annotation
Ordered Locus Names:AF_1112
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003036311 – 323tRNA N6-adenosine threonylcarbamoyltransferaseAdd BLAST323

Interactioni

Subunit structurei

Monomer. Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.UniRule annotation

Protein-protein interaction databases

STRINGi224325.AF1112

Structurei

3D structure databases

ProteinModelPortaliO29153
SMRiO29153
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni126 – 130Substrate bindingUniRule annotation5

Sequence similaritiesi

Belongs to the KAE1 / TsaD family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01183 Archaea
COG0533 LUCA
KOiK01409
OMAiAHAGAEM
OrthoDBiPOG093Z05SV

Family and domain databases

HAMAPiMF_01446 Kae1, 1 hit
InterProiView protein in InterPro
IPR000905 Gcp-like_dom
IPR034680 Kae1/OSGEP
IPR017861 KAE1/TsaD
IPR022449 Kae1_arc
PANTHERiPTHR11735 PTHR11735, 1 hit
PTHR11735:SF14 PTHR11735:SF14, 1 hit
PfamiView protein in Pfam
PF00814 Peptidase_M22, 1 hit
PRINTSiPR00789 OSIALOPTASE
TIGRFAMsiTIGR03722 arch_KAE1, 1 hit
TIGR00329 gcp_kae1, 1 hit

Sequencei

Sequence statusi: Complete.

O29153-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIALGIEGTA WSLSIGVVDE EGVIALENDP YIPKEGGIHP REASQHHSER
60 70 80 90 100
LPSLLSRVFE KVDKNSIDVV AFSQGPGMGP CLRVVATAAR LLAIKLEKPL
110 120 130 140 150
VGVNHCLAHV EVGRWQTGAR KPVSLYVSGG NSQVIARRGN RYRVFGETLD
160 170 180 190 200
IGIGNALDKL ARHMGLKHPG GPKIEELAKK GQKYHFLPYV VKGMDFSFSG
210 220 230 240 250
MVTAAQRLFD SGVRMEDVAF SFQETAFAML TEVTERALAY LDLNEVLLVG
260 270 280 290 300
GVAANKRLQE MLRIMCEDRG AKFYVPPKEL AGDNGAMIAY TGLLMYKHGH
310 320
QTPVEKSYVR PDFRIEDVEV NWD
Length:323
Mass (Da):35,604
Last modified:January 1, 1998 - v1
Checksum:i57373492D614002D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA Translation: AAB90129.1
PIRiG69388
RefSeqiWP_010878608.1, NC_000917.1

Genome annotation databases

EnsemblBacteriaiAAB90129; AAB90129; AF_1112
GeneIDi24794717
KEGGiafu:AF_1112

Similar proteinsi

Entry informationi

Entry nameiKAE1_ARCFU
AccessioniPrimary (citable) accession number: O29153
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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