O29108 (PUR2_ARCFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylamine--glycine ligase
EC=6.3.4.13

Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	AF_1157

Organism

Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)

Taxonomic identifier

224325 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	470 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 470	470	Phosphoribosylamine--glycine ligase HAMAP MF_00138		PRO_0000151508
Regions
	Domain	115 – 354	240	ATP-grasp
	Nucleotide binding	142 – 203	62	ATP By similarity
Sites
	Metal binding	324	1	Magnesium or manganese By similarity
	Metal binding	326	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	O29108 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: C3B90CED22DCD353	FASTA	470	51,750
	10 20 30 40 50 60 MKVLVVDAGG RGNAIAHAFS RSEQVKEIYI APGNGGSEFF EKCKIAELDG KKIPSIRAID 70 80 90 100 110 120 EIVRFAKKCE VDLAYIGPEE PLSLGLVDRL EEEGIPAVGP KKEATILEAS KCWAKDFLKR 130 140 150 160 170 180 IGVPIPEYAN FDNPEEAKEY IREKFNNGIV VKADGLAAGK GVYVCDSVEE ALRAVDEIMV 190 200 210 220 230 240 QKKFGEAGNR IVVEERLRGI EVAFTAMTDG KTVKPFGHAR DYKRAFDSDD IEGLRDFYIG 250 260 270 280 290 300 LTKKFYTKAQ IEQLYREGKL INPNTGGMGA VSPHPAVTEE VEQRIMEMVV EPIIENFDKE 310 320 330 340 350 360 FKGVLYPVIM LVEENGELIP KVLEINVRDC DPGAEAKLPR LKSDMAEISM AVVEGRLDEV 370 380 390 400 410 420 EMRFSSDYCV AVCAVSGALK GREGLKPGYP ADHYTSQPIT GIEEAMKEAI IYANGIAKTN 430 440 450 460 470 GYVTTGGRVL TVVGMGQSIE EARSKAYSAL EKISFPGMRY RRTIGLDVPE

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000782 Genomic DNA. Translation: AAB90089.1.
PIR	D69394.
RefSeq	NP_069986.1. NC_000917.1.
3D structure databases
ProteinModelPortal	O29108.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1484381.
GenomeReviews	Gene locus AF_1157 in contig AE000782_GR.
KEGG	afu:AF1157.
NMPDR	fig|224325.1.peg.1146.
TIGR	AF_1157.
Phylogenomic databases
HOGENOM	HBG404060.
OMA	MGAYTPL.
PhylomeDB	O29108.
ProtClustDB	CLSK541657.
Enzyme and pathway databases
BioCyc	AFUL224325:AF_1157-MONOMER.
Family and domain databases
HAMAP	MF_00138. GARS. Divergent sequence. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020562. PRibGlycinamide_synth_N. IPR011054. Rudment_hybrid_motif. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 2 hits. G3DSA:3.90.600.10. Gars. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
KO	K01945.
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit. SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMs	TIGR00877. PurD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_ARCFU

Accession

Primary (citable) accession number: O29108

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	December 14, 2011
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program