O28997 (PURE_ARCFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N5-carboxyaminoimidazole ribonucleotide mutase
Short name=N5-CAIR mutase
EC=5.4.99.18

Alternative name(s):
5-(carboxyamino)imidazole ribonucleotide mutase

Gene names

Name:	purE
Ordered Locus Names:	AF_1271

Organism

Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)

Taxonomic identifier

224325 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	180 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to 4-carboxy-5-aminoimidazole ribonucleotide (CAIR) By similarity.
Catalytic activity	5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 2/2.
Sequence similarities	Belongs to the AIR carboxylase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	5-(carboxyamino)imidazole ribonucleotide mutase activity Inferred from electronic annotation. Source: EC phosphoribosylaminoimidazole carboxylase activity Inferred from electronic annotation. Source: InterPro phosphoribosylaminoimidazolesuccinocarboxamide synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 180	180	N5-carboxyaminoimidazole ribonucleotide mutase		PRO_0000074986
Sites
	Binding site	35	1	Substrate By similarity
	Binding site	38	1	Substrate By similarity
	Binding site	65	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O28997 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 81F6152E6E14D69D	FASTA	180	20,015
	10 20 30 40 50 60 MSQFLSLRNC LRLTIRLLRQ FRRGEGMKAV IIMGSKSDLD YSKKIASKLA DFGIDAVMRI 70 80 90 100 110 120 ASAHKTPEKV LEIIKEYEKE DVVFVTVAGR SNALSGFVDA NTSKPVIASP PYSDKFGGAD 130 140 150 160 170 180 IFSSIRMPSG VAPMLVLEAE NAALAVAKIF ALKDEGVREK VVQFQENKRR EIYKADEELR

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000782 Genomic DNA. Translation: AAB89973.1.
PIR	F69408.
RefSeq	NP_070099.1. NC_000917.1.
3D structure databases
ProteinModelPortal	O28997.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1484495.
GenomeReviews	Gene locus AF_1271 in contig AE000782_GR.
KEGG	afu:AF1271.
NMPDR	fig|224325.1.peg.1259.
TIGR	AF_1271.
Phylogenomic databases
HOGENOM	HBG301965.
OMA	RMPSGVA.
ProtClustDB	CLSK541688.
Enzyme and pathway databases
BioCyc	AFUL224325:AF_1271-MONOMER.
Family and domain databases
InterPro	IPR024694. N5-CAIR_mutase_PurE. IPR000031. N5-CAIR_Mutase_PurE_dom. IPR001636. SAICAR_synth. [Graphical view]
Gene3D	G3DSA:3.40.50.7700. AIR_carboxyl. 1 hit.
KO	K01588.
PANTHER	PTHR11609. SAICAR_synt. 1 hit.
Pfam	PF00731. AIRC. 1 hit. [Graphical view]
PIRSF	PIRSF001338. AIR_carboxylase. 1 hit.
SMART	SM01001. AIRC. 1 hit. [Graphical view]
SUPFAM	SSF52255. AIR_carboxyl. 1 hit.
TIGRFAMs	TIGR01162. PurE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURE_ARCFU

Accession

Primary (citable) accession number: O28997

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	January 25, 2012
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program