Reviewed, UniProtKB/Swiss-Prot O28965 (TPIS_ARCFU)

Names and origin

Protein names

Recommended name:
    Triosephosphate isomerase
      Short name=TIM
    EC=5.3.1.1
Alternative name(s):
    Triose-phosphate isomerase

Gene names

Name:	tpiA
Ordered Locus Names:	AF_1304

Organism

Archaeoglobus fulgidus [Complete proteome] [HAMAP]

Taxonomic identifier

2234 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	223 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate = glycerone phosphate. HAMAP MF_00147
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00147 Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1. HAMAP MF_00147
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
Biological process	Gluconeogenesis Glycolysis Pentose shunt
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: HAMAP glycolysis Inferred from electronic annotation. Source: HAMAP pentose-phosphate shunt Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triose-phosphate isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 223	223	Triosephosphate isomerase HAMAP MF_00147		PRO_0000090328
Sites
	Active site	94	1	Electrophile By similarity
	Active site	142	1	Proton acceptor By similarity
	Binding site	10	1	Substrate By similarity
	Binding site	12	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O28965-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: CB86A228031A80E9	FASTA	223	23,771
	10 20 30 40 50 60 MRKMGVVIIN FKAYKEGFGK RALELAKIAE NVASRCDEYV GIAVSFLDLP VIAREVSIDV 70 80 90 100 110 120 YAQHVDAVGF GSHTGRINAD MIAEYGAKGS LVNHSERRLK LADIEFNVSR LRELGLTSVV 130 140 150 160 170 180 CTNNVPTTAA AAALNPDFVA VEPPELIGSG IPVSKAEPEV VENSVKAAKE VNKSVRVLCG 190 200 210 220 AGITTHEDYV KALELGAEGV LLASGVVKAE DQKRALEELV GLL

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000782 Genomic DNA. Translation: AAB89944.1.
PIR	G69412.
RefSeq	NP_070133.1.
3D structure databases
HSSP	HSSP built from PDB template 1HG3 based on UniProtKB P95583.
ModBase	Search...
Genome annotation databases
GeneID	1484530.
GenomeReviews	Gene locus AF_1304 in contig AE000782_GR.
KEGG	afu:AF1304.
NMPDR	fig|224325.1.peg.1294.
TIGR	AF_1304.
Phylogenomic databases
HOGENOM	O28965.
OMA	VEPPELI.
Enzyme and pathway databases
BioCyc	AFUL224325:AF_1304-MON.
BRENDA	5.3.1.1. 7576.
Family and domain databases
HAMAP	MF_00147. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR000652. Triosephosphate_isomerase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR21139. Triophos_ismrse. 1 hit.
Pfam	PF00121. TIM. 1 hit. [Graphical view]
ProDom	PD001005. Triophos_ismrse. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00419. tim. 1 hit.
PROSITE	PS00171. TIM_1. 1 hit. PS51440. TIM_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPIS_ARCFU

Accession

Primary (citable) accession number: O28965

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	November 3, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)