Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Piwi protein AF_1318

Gene

AF_1318

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as short interfering RNAs (siRNAs), and may cleave or repress the translation of mRNAs which are complementary to them (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi159Divalent metal cation1
Metal bindingi427Divalent metal cation; via carboxylate1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

RNA-mediated gene silencing

Keywords - Ligandi

Metal-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Piwi protein AF_1318
Alternative name(s):
AfPiwi
Gene namesi
Ordered Locus Names:AF_1318
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi123Y → A: Reduced binding affinity for siRNA. 1 Publication1
Mutagenesisi127K → A: Reduced binding affinity for siRNA. 1 Publication1
Mutagenesisi137Q → A: Reduced binding affinity for siRNA. 1 Publication1
Mutagenesisi163K → A: Reduced binding affinity for siRNA. 1 Publication1
Mutagenesisi427L → LG: Reduced binding to siRNA. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003781461 – 427Piwi protein AF_1318Add BLAST427

Interactioni

Protein-protein interaction databases

STRINGi224325.AF1318.

Structurei

Secondary structure

1427
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 6Combined sources4
Beta strandi12 – 14Combined sources3
Helixi16 – 18Combined sources3
Beta strandi20 – 24Combined sources5
Helixi26 – 36Combined sources11
Beta strandi45 – 54Combined sources10
Helixi59 – 74Combined sources16
Beta strandi82 – 87Combined sources6
Beta strandi89 – 92Combined sources4
Helixi93 – 106Combined sources14
Beta strandi109 – 117Combined sources9
Helixi120 – 131Combined sources12
Beta strandi136 – 140Combined sources5
Helixi141 – 143Combined sources3
Turni146 – 148Combined sources3
Helixi149 – 163Combined sources15
Helixi175 – 177Combined sources3
Beta strandi180 – 202Combined sources23
Beta strandi208 – 213Combined sources6
Helixi219 – 221Combined sources3
Helixi222 – 240Combined sources19
Beta strandi247 – 256Combined sources10
Helixi261 – 277Combined sources17
Beta strandi286 – 294Combined sources9
Beta strandi298 – 301Combined sources4
Turni304 – 307Combined sources4
Beta strandi313 – 328Combined sources16
Beta strandi332 – 334Combined sources3
Beta strandi340 – 353Combined sources14
Turni357 – 359Combined sources3
Helixi360 – 375Combined sources16
Beta strandi381 – 383Combined sources3
Helixi389 – 406Combined sources18
Turni414 – 416Combined sources3
Helixi419 – 422Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1W9HX-ray1.95A1-427[»]
1YTUX-ray2.50A/B1-427[»]
2BGGX-ray2.20A/B1-427[»]
2W42X-ray1.90A/B1-427[»]
ProteinModelPortaliO28951.
SMRiO28951.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO28951.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini110 – 406PiwiPROSITE-ProRule annotationAdd BLAST297

Sequence similaritiesi

Belongs to the argonaute family.Curated
Contains 1 Piwi domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiarCOG03890. Archaea.
COG1431. LUCA.
KOiK07578.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR003165. Piwi.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF02171. Piwi. 1 hit.
[Graphical view]
SMARTiSM00950. Piwi. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
PROSITEiPS50822. PIWI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O28951-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMEYKIVENG LTYRIGNGAS VPISNTGELI KGLRNYGPYE VPSLKYNQIA
60 70 80 90 100
LIHNNQFSSL INQLKSQISS KIDEVWHIHN INISEFIYDS PHFDSIKSQV
110 120 130 140 150
DNAIDTGVDG IMLVLPEYNT PLYYKLKSYL INSIPSQFMR YDILSNRNLT
160 170 180 190 200
FYVDNLLVQF VSKLGGKPWI LNVDPEKGSD IIIGTGATRI DNVNLFCFAM
210 220 230 240 250
VFKKDGTMLW NEISPIVTSS EYLTYLKSTI KKVVYGFKKS NPDWDVEKLT
260 270 280 290 300
LHVSGKRPKM KDGETKILKE TVEELKKQEM VSRDVKYAIL HLNETHPFWV
310 320 330 340 350
MGDPNNRFHP YEGTKVKLSS KRYLLTLLQP YLKRNGLEMV TPIKPLSVEI
360 370 380 390 400
VSDNWTSEEY YHNVHEILDE IYYLSKMNWR GFRSRNLPVT VNYPKLVAGI
410 420
IANVNRYGGY PINPEGNRSL QTNPWFL
Length:427
Mass (Da):49,244
Last modified:January 1, 1998 - v1
Checksum:iF925081F3AFB47CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB89939.1.
PIRiE69414.

Genome annotation databases

EnsemblBacteriaiAAB89939; AAB89939; AF_1318.
KEGGiafu:AF_1318.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB89939.1.
PIRiE69414.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1W9HX-ray1.95A1-427[»]
1YTUX-ray2.50A/B1-427[»]
2BGGX-ray2.20A/B1-427[»]
2W42X-ray1.90A/B1-427[»]
ProteinModelPortaliO28951.
SMRiO28951.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224325.AF1318.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB89939; AAB89939; AF_1318.
KEGGiafu:AF_1318.

Phylogenomic databases

eggNOGiarCOG03890. Archaea.
COG1431. LUCA.
KOiK07578.

Miscellaneous databases

EvolutionaryTraceiO28951.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR003165. Piwi.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF02171. Piwi. 1 hit.
[Graphical view]
SMARTiSM00950. Piwi. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
PROSITEiPS50822. PIWI. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPIWI_ARCFU
AccessioniPrimary (citable) accession number: O28951
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.