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Protein

Piwi protein AF_1318

Gene

AF_1318

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as short interfering RNAs (siRNAs), and may cleave or repress the translation of mRNAs which are complementary to them (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi159 – 1591Divalent metal cation
Metal bindingi427 – 4271Divalent metal cation; via carboxylate

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

RNA-mediated gene silencing

Keywords - Ligandi

Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-1348-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Piwi protein AF_1318
Alternative name(s):
AfPiwi
Gene namesi
Ordered Locus Names:AF_1318
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi123 – 1231Y → A: Reduced binding affinity for siRNA. 1 Publication
Mutagenesisi127 – 1271K → A: Reduced binding affinity for siRNA. 1 Publication
Mutagenesisi137 – 1371Q → A: Reduced binding affinity for siRNA. 1 Publication
Mutagenesisi163 – 1631K → A: Reduced binding affinity for siRNA. 1 Publication
Mutagenesisi427 – 4271L → LG: Reduced binding to siRNA. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 427427Piwi protein AF_1318PRO_0000378146Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi224325.AF1318.

Structurei

Secondary structure

1
427
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 64Combined sources
Beta strandi12 – 143Combined sources
Helixi16 – 183Combined sources
Beta strandi20 – 245Combined sources
Helixi26 – 3611Combined sources
Beta strandi45 – 5410Combined sources
Helixi59 – 7416Combined sources
Beta strandi82 – 876Combined sources
Beta strandi89 – 924Combined sources
Helixi93 – 10614Combined sources
Beta strandi109 – 1179Combined sources
Helixi120 – 13112Combined sources
Beta strandi136 – 1405Combined sources
Helixi141 – 1433Combined sources
Turni146 – 1483Combined sources
Helixi149 – 16315Combined sources
Helixi175 – 1773Combined sources
Beta strandi180 – 20223Combined sources
Beta strandi208 – 2136Combined sources
Helixi219 – 2213Combined sources
Helixi222 – 24019Combined sources
Beta strandi247 – 25610Combined sources
Helixi261 – 27717Combined sources
Beta strandi286 – 2949Combined sources
Beta strandi298 – 3014Combined sources
Turni304 – 3074Combined sources
Beta strandi313 – 32816Combined sources
Beta strandi332 – 3343Combined sources
Beta strandi340 – 35314Combined sources
Turni357 – 3593Combined sources
Helixi360 – 37516Combined sources
Beta strandi381 – 3833Combined sources
Helixi389 – 40618Combined sources
Turni414 – 4163Combined sources
Helixi419 – 4224Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1W9HX-ray1.95A1-427[»]
1YTUX-ray2.50A/B1-427[»]
2BGGX-ray2.20A/B1-427[»]
2W42X-ray1.90A/B1-427[»]
ProteinModelPortaliO28951.
SMRiO28951. Positions 1-427.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO28951.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini110 – 406297PiwiPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the argonaute family.Curated
Contains 1 Piwi domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiarCOG03890. Archaea.
COG1431. LUCA.
KOiK07578.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR003165. Piwi.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF02171. Piwi. 1 hit.
[Graphical view]
SMARTiSM00950. Piwi. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
PROSITEiPS50822. PIWI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O28951-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMEYKIVENG LTYRIGNGAS VPISNTGELI KGLRNYGPYE VPSLKYNQIA
60 70 80 90 100
LIHNNQFSSL INQLKSQISS KIDEVWHIHN INISEFIYDS PHFDSIKSQV
110 120 130 140 150
DNAIDTGVDG IMLVLPEYNT PLYYKLKSYL INSIPSQFMR YDILSNRNLT
160 170 180 190 200
FYVDNLLVQF VSKLGGKPWI LNVDPEKGSD IIIGTGATRI DNVNLFCFAM
210 220 230 240 250
VFKKDGTMLW NEISPIVTSS EYLTYLKSTI KKVVYGFKKS NPDWDVEKLT
260 270 280 290 300
LHVSGKRPKM KDGETKILKE TVEELKKQEM VSRDVKYAIL HLNETHPFWV
310 320 330 340 350
MGDPNNRFHP YEGTKVKLSS KRYLLTLLQP YLKRNGLEMV TPIKPLSVEI
360 370 380 390 400
VSDNWTSEEY YHNVHEILDE IYYLSKMNWR GFRSRNLPVT VNYPKLVAGI
410 420
IANVNRYGGY PINPEGNRSL QTNPWFL
Length:427
Mass (Da):49,244
Last modified:January 1, 1998 - v1
Checksum:iF925081F3AFB47CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB89939.1.
PIRiE69414.

Genome annotation databases

EnsemblBacteriaiAAB89939; AAB89939; AF_1318.
KEGGiafu:AF_1318.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB89939.1.
PIRiE69414.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1W9HX-ray1.95A1-427[»]
1YTUX-ray2.50A/B1-427[»]
2BGGX-ray2.20A/B1-427[»]
2W42X-ray1.90A/B1-427[»]
ProteinModelPortaliO28951.
SMRiO28951. Positions 1-427.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224325.AF1318.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB89939; AAB89939; AF_1318.
KEGGiafu:AF_1318.

Phylogenomic databases

eggNOGiarCOG03890. Archaea.
COG1431. LUCA.
KOiK07578.

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-1348-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO28951.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR003165. Piwi.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF02171. Piwi. 1 hit.
[Graphical view]
SMARTiSM00950. Piwi. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
PROSITEiPS50822. PIWI. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPIWI_ARCFU
AccessioniPrimary (citable) accession number: O28951
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: January 1, 1998
Last modified: February 17, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.