Reviewed, UniProtKB/Swiss-Prot O28949 (GUAAA_ARCFU)

Names and origin

Protein names

Recommended name:
    GMP synthase [glutamine-hydrolyzing] subunit A
    EC=6.3.5.2
Alternative name(s):
    Glutamine amidotransferase

Gene names

Name:	guaAA
Ordered Locus Names:	AF_1320

Organism

Archaeoglobus fulgidus [Complete proteome] [HAMAP]

Taxonomic identifier

2234 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	183 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity.
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_01510
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_01510
Subunit structure	Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 183	183	GMP synthase [glutamine-hydrolyzing] subunit A HAMAP MF_01510		PRO_0000140217
Regions
	Domain	2 – 183	182	Glutamine amidotransferase type-1
Sites
	Active site	74	1	Nucleophile By similarity
	Active site	161	1	By similarity
	Active site	163	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O28949-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: BA11A3E31ABF6C56	FASTA	183	20,884
	10 20 30 40 50 60 MKIYVIYNYG QYNHLIHRTL RDLGVETKLV ENTTPVEQLK DVDGLVIGGG PSLDRTGNCE 70 80 90 100 110 120 LYLKELDVPM IGICLGHQLM AKVFGGEVGK GSMGGYSEVK VRIVEDDELF EGIPREITVW 130 140 150 160 170 180 ASHMDEVKKL PEGFKRLAES DICKIEAMRH EKKPLYGVQW HPEVYHSQFG VELYRNFIEI CKK

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000782 Genomic DNA. Translation: AAB89925.1.
PIR	G69414.
RefSeq	NP_070149.1.
3D structure databases
HSSP	HSSP built from PDB template 1GPM based on UniProtKB P04079.
ModBase	Search...
Genome annotation databases
GeneID	1484546.
GenomeReviews	Gene locus AF_1320 in contig AE000782_GR.
KEGG	afu:AF1320.
NMPDR	fig|224325.1.peg.1310.
TIGR	AF_1320.
Phylogenomic databases
HOGENOM	O28949.
OMA	GQYVHRI.
Enzyme and pathway databases
BioCyc	AFUL224325:AF_1320-MON.
BRENDA	6.3.5.2. 7576.
Family and domain databases
HAMAP	MF_01510. [Tree]
InterPro	IPR006220. Anth_synthII. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. IPR004739. GMP_synth_N. [Graphical view]
Pfam	PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00096. GATASE.
TIGRFAMs	TIGR00888. guaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAAA_ARCFU

Accession

Primary (citable) accession number: O28949

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	January 1, 1998
Last modified:	November 3, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)