O28853 (KPRS2_ARCFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribose-phosphate pyrophosphokinase 2
Short name=RPPK 2
EC=2.7.6.1

Alternative name(s):
Phosphoribosyl pyrophosphate synthase 2
Short name=P-Rib-PP synthase 2
Short name=PRPP synthase 2

Gene names

Name:	prs2
Synonyms:	prsA-2
Ordered Locus Names:	AF_1419

Organism

Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)

Taxonomic identifier

224325 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00583_A
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00583_A
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP MF_00583_A
Subcellular location	Cytoplasm By similarity HAMAP MF_00583_A.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 271	271	Ribose-phosphate pyrophosphokinase 2 HAMAP MF_00583_A		PRO_0000141235
Regions
	Region	192 – 205	14	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	113	1	Magnesium Potential
	Metal binding	115	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	O28853 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 1685B4364B254D17	FASTA	271	29,059
	10 20 30 40 50 60 MKIIPCPSSP LLARRVAEAA GIEIGGAVFK KFPDGELYVR VMEKEGVVVG SINSNEDLIA 70 80 90 100 110 120 LIFALDVLEN AKAVVPYMGY ARQDKVFQEG EAVSIKIVAE MLESRADEVV TVNIHSSDAA 130 140 150 160 170 180 SHFSKLKNLD AMPLVGEYFA GKDVVMISPD KGSMERVKTA AKHAGCEWDY MEKRRIDATT 190 200 210 220 230 240 VEITPKTIDV EGRDVVIVDD IISTGGTVAE AARILYGLGA KSVSAACVHA VLAENAAIKL 250 260 270 FNAGIKDIIA TDTVECAFSR ISVAELIAEN I

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000782 Genomic DNA. Translation: AAB89836.1.
PIR	B69427.
RefSeq	NP_070248.1. NC_000917.1.
3D structure databases
ProteinModelPortal	O28853.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1484643.
GenomeReviews	Gene locus AF_1419 in contig AE000782_GR.
KEGG	afu:AF1419.
NMPDR	fig|224325.1.peg.1409.
TIGR	AF_1419.
Phylogenomic databases
HOGENOM	HBG519284.
OMA	TRISPTE.
ProtClustDB	CLSK2746896.
Enzyme and pathway databases
BioCyc	AFUL224325:AF_1419-MONOMER.
Family and domain databases
HAMAP	MF_00583_A. RibP_PPkinase_A. [Tree]
InterPro	IPR000836. PRibTrfase. IPR005946. Rib-P_diPkinase. [Graphical view]
KO	K00948.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. RibP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS2_ARCFU

Accession

Primary (citable) accession number: O28853

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	January 1, 1998
Last modified:	November 16, 2011
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program