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O28837 (RTCA_ARCFU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RNA 3'-terminal phosphate cyclase

Short name=RNA cyclase
Short name=RNA-3'-phosphate cyclase
EC=6.5.1.4
Gene names
Name:rtcA
Ordered Locus Names:AF_1435
OrganismArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) [Reference proteome] [HAMAP]
Taxonomic identifier224325 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus

Protein attributes

Sequence length328 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity. HAMAP-Rule MF_00200

Catalytic activity

ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP-Rule MF_00200

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00200.

Sequence similarities

Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processRNA processing

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA-3'-phosphate cyclase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 328328RNA 3'-terminal phosphate cyclase HAMAP-Rule MF_00200
PRO_0000156423

Regions

Nucleotide binding276 – 2805ATP By similarity

Sites

Active site3021Tele-AMP-histidine intermediate By similarity
Binding site1001ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
O28837 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: A3B6229111098AA4

FASTA32835,454
        10         20         30         40         50         60 
MIEIDGSFGE GGGQILRTAV ALSCVTGKAV RIRNIRANRP KPGLAAQHLK GIEAAKIISN 

        70         80         90        100        110        120 
AEVEGLRIGS TEIVFNPGSV RGGNFRVDIG TAGSVTLIFQ TVLLPLLFAD RDSTLTVTGG 

       130        140        150        160        170        180 
TDVAWAPPVD YFKNVTLRAL REMGAECELE VLKRGYYPKG GGMVRLTVRP AEMKGVVYER 

       190        200        210        220        230        240 
IDEIVRGVSH CQNLPGHVAE RQASSARNFL EERGIRAEIR TEVLKGLSTG SGIVLWSGYK 

       250        260        270        280        290        300 
GGSALGERGK RAEVVGQEAA ESLYRELMSD AAFDAHLADQ VMPFAAMARG RTEYTTSEVT 

       310        320 
MHQKSNAYVI NTFLGKVVKF EGNKIIIK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000782 Genomic DNA. Translation: AAB89810.1.
PIRB69429.
RefSeqNP_070264.1. NC_000917.1.

3D structure databases

ProteinModelPortalO28837.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224325.AF1435.

Proteomic databases

PRIDEO28837.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB89810; AAB89810; AF_1435.
GeneID1484659.
KEGGafu:AF1435.

Phylogenomic databases

eggNOGCOG0430.
KOK01974.
OMARRGHYPK.
ProtClustDBPRK04204.

Enzyme and pathway databases

BioCycAFUL224325:GJBC-1466-MONOMER.

Family and domain databases

Gene3D3.30.360.20. 1 hit.
3.65.10.20. 2 hits.
HAMAPMF_00200. RTC.
InterProIPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR017770. RNA3'_term_phos_cyc_type_1.
IPR020719. RNA3'_term_phos_cycl-like_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERPTHR11096. PTHR11096. 1 hit.
PfamPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
SUPFAMSSF55205. SSF55205. 1 hit.
TIGRFAMsTIGR03399. RNA_3prim_cycl. 1 hit.
PROSITEPS01287. RTC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRTCA_ARCFU
AccessionPrimary (citable) accession number: O28837
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: February 19, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families