Reviewed, UniProtKB/Swiss-Prot O28837 (RTCA_ARCFU)

Names and origin

Protein names

Recommended name:
    Probable RNA 3'-terminal phosphate cyclase
      Short name=RNA-3'-phosphate cyclase
      Short name=RNA cyclase
    EC=6.5.1.4

Gene names

Name:	rtcA
Ordered Locus Names:	AF_1435

Organism

Archaeoglobus fulgidus [Complete proteome] [HAMAP]

Taxonomic identifier

2234 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) the enzyme acts on RNA-N3'P to produce RNA-N3'PP5'A; (C) a non catalytic nucleophilic attack by the adjacent 2'hydroxyl on the phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity.
Catalytic activity	ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP MF_00200
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-3'-phosphate cyclase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	Probable RNA 3'-terminal phosphate cyclase HAMAP MF_00200		PRO_0000156423
Sites
	Active site	302	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O28837-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: A3B6229111098AA4	FASTA	328	35,454
	10 20 30 40 50 60 MIEIDGSFGE GGGQILRTAV ALSCVTGKAV RIRNIRANRP KPGLAAQHLK GIEAAKIISN 70 80 90 100 110 120 AEVEGLRIGS TEIVFNPGSV RGGNFRVDIG TAGSVTLIFQ TVLLPLLFAD RDSTLTVTGG 130 140 150 160 170 180 TDVAWAPPVD YFKNVTLRAL REMGAECELE VLKRGYYPKG GGMVRLTVRP AEMKGVVYER 190 200 210 220 230 240 IDEIVRGVSH CQNLPGHVAE RQASSARNFL EERGIRAEIR TEVLKGLSTG SGIVLWSGYK 250 260 270 280 290 300 GGSALGERGK RAEVVGQEAA ESLYRELMSD AAFDAHLADQ VMPFAAMARG RTEYTTSEVT 310 320 MHQKSNAYVI NTFLGKVVKF EGNKIIIK

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000782 Genomic DNA. Translation: AAB89810.1.
PIR	B69429.
RefSeq	NP_070264.1.
3D structure databases
HSSP	HSSP built from PDB template 1QMH based on UniProtKB P46849.
ModBase	Search...
Genome annotation databases
GeneID	1484659.
GenomeReviews	Gene locus AF_1435 in contig AE000782_GR.
KEGG	afu:AF1435.
NMPDR	fig|224325.1.peg.1425.
TIGR	AF_1435.
Phylogenomic databases
HOGENOM	O28837.
OMA	EIDGSYG.
Enzyme and pathway databases
BioCyc	AFUL224325:AF_1435-MON.
BRENDA	6.5.1.4. 7576.
Family and domain databases
HAMAP	MF_00200. [Tree]
InterPro	IPR000228. RNA3'_term_phos_cycl-like. IPR013796. RNA3'_term_phos_cycl_insert. IPR017770. RNA3'_term_phos_cycl_sub. [Graphical view]
Gene3D	G3DSA:3.65.10.20. RNA3'_term_phos_cycl. 1 hit.
PANTHER	PTHR11096. RNA3'_term_phos_cycl. 1 hit.
Pfam	PF01137. RTC. 1 hit. PF05189. RTC_insert. 1 hit. [Graphical view]
TIGRFAMs	TIGR03399. RNA_3prim_cycl. 1 hit.
PROSITE	PS01287. RTC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RTCA_ARCFU

Accession

Primary (citable) accession number: O28837

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	November 3, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)