Reviewed, UniProtKB/Swiss-Prot O28819 (SYM_ARCFU)

Names and origin

Protein names

Recommended name:
    Methionyl-tRNA synthetase
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	AF_1453

Organism

Archaeoglobus fulgidus [Complete proteome] [HAMAP]

Taxonomic identifier

2234 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	658 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity.
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00098
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 658	658	Methionyl-tRNA synthetase HAMAP MF_00098		PRO_0000139183
Regions
	Domain	558 – 658	101	tRNA-binding
	Motif	9 – 19	11	"HIGH" region HAMAP MF_00098
	Motif	322 – 326	5	"KMSKS" region HAMAP MF_00098
Sites
	Metal binding	140	1	Zinc By similarity
	Metal binding	143	1	Zinc By similarity
	Metal binding	152	1	Zinc By similarity
	Metal binding	156	1	Zinc By similarity
	Binding site	325	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	O28819-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: CEDCD417AFB95F1C	FASTA	658	75,695
	10 20 30 40 50 60 MKLVTCGLPY ANGKAHVGHL RTYVPADVYV RYLRMSGEEV VFVCGSDCHG TPIVVNAEQQ 70 80 90 100 110 120 GLSPKELVDI YHEHFIKIFD ALNIKFDFYG RTDSDYHHHR TTEIVKRLIE KGYVYPKEIQ 130 140 150 160 170 180 LAYCPKCQRF LPDRYVEGIC PYCGALARGD ECDQGCGRHL EPGEIKEPRC KICGSKAEFR 190 200 210 220 230 240 SQRHYFFKLT EFQDFLEDYL SKLKGTENAL NYARNWVKNL RDWCITRNLE WGVRFPGEPN 250 260 270 280 290 300 LVVYVWVDAP IGYISFTEKA CEEKGCDWRK IWIDGDAEII HFIGLDIVYH HCIFWPAMLK 310 320 330 340 350 360 GADYALPSAV VASGMVKVEG KTFSKSRGYV VWVEEDYLKS GLSPDYLRYY IVNYTSHQKD 370 380 390 400 410 420 LNFSWEVFRE KVNNEVIATL GNFLYRVLLF AWKNFGEVRM EGVDEEVLEK IRETEQKILS 430 440 450 460 470 480 ALEEWEFKAA SDAFMELATY GNVYFQNAKP WELIKTDKEA ARRAVASCLQ LAKALIIFAY 490 500 510 520 530 540 PVMPESMERM AKAIGLDLEN VRLSDAYKVD EVMKLSKPEV PFAKVEDEVI EKLQKVMEKR 550 560 570 580 590 600 FRGEGEKMEQ KEEISIEDFQ KLDIRIGRVL KAEKVKKSKK LIKLIIDIGD EQRQIVSGIA 610 620 630 640 650 EDYTPEELEG KLVVVLANLK PAKFMGVESR GMILAAEKDG KAVLLTPEKE VEPGTRVC

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000782 Genomic DNA. Translation: AAB89796.1.
PIR	D69431.
RefSeq	NP_070282.1.
3D structure databases
HSSP	HSSP built from PDB template 1MKH based on UniProtKB Q9V011.
ModBase	Search...
Genome annotation databases
GeneID	1484677.
GenomeReviews	Gene locus AF_1453 in contig AE000782_GR.
KEGG	afu:AF1453.
NMPDR	fig|224325.1.peg.1443.
TIGR	AF_1453.
Phylogenomic databases
HOGENOM	O28819.
OMA	SAYQPEQ.
Enzyme and pathway databases
BioCyc	AFUL224325:AF_1453-MON.
BRENDA	6.1.1.10. 7576.
Family and domain databases
HAMAP	MF_00098. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR002304. Met-tRNA-synth_Ia. IPR004495. Met-tRNA-synth_Ia_bsu_C. IPR012340. NA-bd_OB-fold. IPR014729. Rossmann-like_a/b/a_fold. IPR014758. tRNA-synt_met_N. IPR002547. tRNA_bd. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF09334. tRNA-synt_1g. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit. TIGR00399. metG_C_term. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_ARCFU

Accession

Primary (citable) accession number: O28819

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	November 3, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)