Reviewed, UniProtKB/Swiss-Prot O28766 (DHAS_ARCFU)

Names and origin

Protein names

Recommended name:
    Aspartate-semialdehyde dehydrogenase
      Short name=ASA dehydrogenase
      Short name=ASADH
    EC=1.2.1.11

Gene names

Name:	asd
Ordered Locus Names:	AF_1506

Organism

Archaeoglobus fulgidus [Complete proteome] [HAMAP]

Taxonomic identifier

2234 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This enzyme catalyzes the second step in the common metabolic pathway to synthesize Thr and Met from Asp.
Catalytic activity	L-aspartate 4-semialdehyde + phosphate + NADP+ = L-4-aspartyl phosphate + NADPH.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; tetrahydrodipicolinate from L-aspartate: step 2/4. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 2/3. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 2/5.
Sequence similarities	Belongs to the aspartate-semialdehyde dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis Threonine biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW threonine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro aspartate-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 343	343	Aspartate-semialdehyde dehydrogenase		PRO_0000141396
Sites
	Active site	148	1	Acyl-thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O28766-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: A6C9F5D045CBFC21	FASTA	343	37,697
	10 20 30 40 50 60 MRYSVGVLGA TGMVGQKFIQ MLAEHPWFKL TSLAASERRV GKKYGEEVDW IVSREVPDIA 70 80 90 100 110 120 KDIEMVPMDP KHVDADIVFS ALPSDIAREV EPKFAEAGFV VASNASAYRM AEDVPLVIPE 130 140 150 160 170 180 VNPEHLGLIE VQKKNRGWDG FIVTNPNCTT IVLVLSLKPL MDLGLRTVRV ASMQALSGAG 190 200 210 220 230 240 YPGVPSLAIT DNVIPFIKGE EDKVEEEPLK LLGKFNGRKI EFADIKVSAS CHRVPVIDGH 250 260 270 280 290 300 TEAVWVEFDR EVSVEEAKAA FESLKPLDLP TSPEKVIIVR EEPDRPQPRL DRDAGNGMSI 310 320 330 340 TVGRIRKDGE RGLKYIVLGH NTVRGAAGAS ILNAELMIKE KII

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000782 Genomic DNA. Translation: AAB89738.1.
PIR	A69438.
RefSeq	NP_070335.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1484733.
GenomeReviews	Gene locus AF_1506 in contig AE000782_GR.
KEGG	afu:AF1506.
NMPDR	fig|224325.1.peg.1496.
TIGR	AF_1506.
Phylogenomic databases
HOGENOM	O28766.
OMA	O28766. TIGAVIS.
Enzyme and pathway databases
BioCyc	AFUL224325:AF_1506-MON.
BRENDA	1.2.1.11. 7576.
Family and domain databases
InterPro	IPR000319. Asp-semialdehyde_DH_CS. IPR005676. Asp_semi-ald_DH_pep-lack. IPR012080. Asp_semialdehyde_DH. IPR000534. Semialdehyde_DH_NAD-bd. IPR012280. Semialdhyde_DH_C. [Graphical view]
Pfam	PF01118. Semialdhyde_dh. 1 hit. PF02774. Semialdhyde_dhC. 1 hit. [Graphical view]
PIRSF	PIRSF000148. ASA_dh. 1 hit.
TIGRFAMs	TIGR00978. asd_EA. 1 hit.
PROSITE	PS01103. ASD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHAS_ARCFU

Accession

Primary (citable) accession number: O28766

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)