Reviewed, UniProtKB/Swiss-Prot O28664 (SYP_ARCFU)

Names and origin

Protein names

Recommended name:
    Prolyl-tRNA synthetase
    EC=6.1.1.15
Alternative name(s):
    Proline--tRNA ligase
      Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	AF_1609

Organism

Archaeoglobus fulgidus [Complete proteome] [HAMAP]

Taxonomic identifier

2234 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity.
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01571
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP proline-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 471	471	Prolyl-tRNA synthetase HAMAP MF_01571		PRO_0000139350

Sequences

	Sequence		Length	Mass (Da)
	O28664-1 [UniParc]. Last modified September 5, 2006. Version 2. Checksum: E730F9B2BED26F00	FASTA	471	54,521
	10 20 30 40 50 60 MTLPSKENFS EWYHEVIQTA EIMDVRYPVK GLYVWFPFGF KIRQLVYSKL REIMDREHDE 70 80 90 100 110 120 VYFPALIPET ELGKEGEHIK GFEDEVYWIT HGGLTPLDVK LALRPTSETA MYPMFRLWVR 130 140 150 160 170 180 NHADLPLKVY QIVNTFRYET KHTRPLIRLR EITSFKEAHT VHKDFEEAAE HVKKAIGFYK 190 200 210 220 230 240 EFYDFLAIPY MVIRRPEWDK FPGAAYTIAF DTIMPDGRTL QIGTVHHLAD NFARTFDIKY 250 260 270 280 290 300 EAPNGEHYYA HQTCYGISER CIAALISVHG DDLGLVLPFE VAPVQIVIIP ILYKGKEEAV 310 320 330 340 350 360 MEACRKLAEK LKAFRVVLDE GEDRPGAKYY KWELKGVPIR FEIGPRDAEK GVAVVSFRDE 370 380 390 400 410 420 KRKFEVPLDE VDEGKVLEWA RELKQRLRNA AAERMAEKVK FVENTGEIEE WGGVVAVYLC 430 440 450 460 470 SDEDCGHSVE EHGKSLLGWF EEVPDWLDLE PEGKCVVCGR EGRLAALAKT Y

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000782 Genomic DNA. Translation: AAB89637.1. Different initiation.
PIR	H69450.
RefSeq	NP_070438.1.
3D structure databases
HSSP	HSSP built from PDB template 1NJ1 based on UniProtKB O26708.
ModBase	Search...
Genome annotation databases
GeneID	1484834.
GenomeReviews	Gene locus AF_1609 in contig AE000782_GR.
KEGG	afu:AF1609.
NMPDR	fig|224325.1.peg.1599.
TIGR	AF_1609.
Phylogenomic databases
HOGENOM	O28664.
Enzyme and pathway databases
BioCyc	AFUL224325:AF_1609-MON.
BRENDA	6.1.1.15. 7576.
Family and domain databases
HAMAP	MF_01571. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR002316. Pro-tRNA-synth_IIa_cons-reg. IPR004499. Pro-tRNA-synth_IIa_pro-type. IPR016061. Pro-tRNA_synth_II_C. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.30.110.30. Pro-tRNA-synth_II_C_arc/euk. 1 hit.
PANTHER	PTHR11451:SF6. ProS_fam_I. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
TIGRFAMs	TIGR00408. proS_fam_I. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_ARCFU

Accession

Primary (citable) accession number: O28664

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)