O28604 (O28604_ARCFU) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Adenylylsulfate reductase, subunit B (AprB) EMBL AAB89580.1

Gene names

Ordered Locus Names:

AF_1669

Organism

Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) [Reference proteome] [HAMAP] EMBL AAB89580.1

Taxonomic identifier

224325 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus › Archaeoglobus fulgidus

Protein attributes

Sequence length	150 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	4Fe-4S RuleBase RU003429 PDB 1JNZ PDB 2FJA PDB 2FJD PDB 2FJE PDB 2FJB PDB 1JNR Iron Iron-sulfur Metal-binding
Technical term	3D-structure PDB 1JNZ PDB 2FJA PDB 2FJD PDB 2FJE PDB 2FJB PDB 1JNR Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	3	1	Iron-sulfur (4Fe-4S) 1 PDB 1JNZ PDB 2FJA PDB 2FJD PDB 2FJE
	Metal binding	10	1	Iron-sulfur (4Fe-4S) 2 PDB 1JNZ PDB 2FJA PDB 2FJD PDB 2FJE PDB 2FJB PDB 1JNR
	Metal binding	11	1	Iron-sulfur (4Fe-4S) 2 PDB 1JNZ PDB 2FJA PDB 2FJD PDB 2FJE PDB 2FJB PDB 1JNR
	Metal binding	13	1	Iron-sulfur (4Fe-4S) 2 PDB 1JNZ PDB 2FJA PDB 2FJD PDB 2FJE PDB 2FJB PDB 1JNR
	Metal binding	21	1	Iron-sulfur (4Fe-4S) 2 PDB 1JNZ PDB 2FJA PDB 2FJD PDB 2FJE PDB 2FJB PDB 1JNR
	Metal binding	25	1	Iron-sulfur (4Fe-4S) 1 PDB 1JNZ PDB 2FJA PDB 2FJD PDB 2FJE PDB 2FJB PDB 1JNR
	Metal binding	47	1	Iron-sulfur (4Fe-4S) 1 PDB 1JNZ PDB 2FJA PDB 2FJD PDB 2FJE PDB 2FJB PDB 1JNR
	Metal binding	50	1	Iron-sulfur (4Fe-4S) 1 PDB 1JNZ PDB 2FJA PDB 2FJD PDB 2FJE PDB 2FJB PDB 1JNR
	Metal binding	53	1	Iron-sulfur (4Fe-4S) 1 PDB 1JNZ PDB 2FJA PDB 2FJD PDB 2FJE PDB 2FJB PDB 1JNR
	Metal binding	57	1	Iron-sulfur (4Fe-4S) 2 PDB 1JNZ PDB 2FJA PDB 2FJD PDB 2FJE PDB 2FJB PDB 1JNR
	Metal binding	58	1	Iron-sulfur (4Fe-4S) 2 PDB 2FJA
	Metal binding	62	1	Iron-sulfur (4Fe-4S) 2 PDB 2FJE
	Site	48	1	Important for catalytic activity

Sequences

	Sequence		Length	Mass (Da)
	O28604 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 5136A55608ADC183	FASTA	150	16,938
	10 20 30 40 50 60 MPSFVNPEKC DGCKALERTA CEYICPNDLM TLDKEKMKAY NREPDMCWEC YSCVKMCPQG 70 80 90 100 110 120 AIDVRGYVDY SPLGGACVPM RGTSDIMWTV KYRNGKVLRF KFAIRTTPWG SIQPFEGFPE 130 140 150 PTEEALKSEL LAGEPEIIGT SEFPQVKKKA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.
[2]	"Structure of adenylylsulfate reductase from the hyperthermophilic Archaeoglobus fulgidus at 1.6-A resolution." Fritz G., Roth A., Schiffer A., Buchert T., Bourenkov G., Bartunik H.D., Huber H., Stetter K.O., Kroneck P.M., Ermler U. Proc. Natl. Acad. Sci. U.S.A. 99:1836-1841(2002) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) IN COMPLEX WITH IRON-SULFUR (4FE-4S), ACTIVE SITE.
[3]	"Reaction mechanism of the iron-sulfur flavoenzyme adenosine-5'-phosphosulfate reductase based on the structural characterization of different enzymatic states." Schiffer A., Fritz G., Kroneck P.M., Ermler U. Biochemistry 45:2960-2967(2006) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) IN COMPLEX WITH IRON-SULFUR (4FE-4S).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE000782 Genomic DNA. Translation: AAB89580.1.

PIR

D69458.

RefSeq

NP_070497.1. NC_000917.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1JNR	X-ray	1.60	B/D	1-150	[»]
1JNZ	X-ray	2.50	B/D	1-150	[»]
2FJA	X-ray	2.00	B/D	1-150	[»]
2FJB	X-ray	1.70	B/D	1-150	[»]
2FJD	X-ray	1.84	B/D	1-150	[»]
2FJE	X-ray	1.80	B/D	1-150	[»]

ProteinModelPortal

O28604.

SMR

O28604. Positions 2-150.

ModBase

Search...

Protein-protein interaction databases

STRING

224325.AF1669.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAB89580; AAB89580; AF_1669.

GeneID

1484892.

KEGG

afu:AF1669.

Phylogenomic databases

eggNOG

COG1146.

KO

K00395.

OMA

CPNDLMV.

ProtClustDB

CLSK2746986.

Enzyme and pathway databases

BioCyc

AFUL224325:GJBC-1703-MONOMER.

Family and domain databases

InterPro

IPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR011802. AprB.
IPR022738. AprB_C.
[Graphical view]

Pfam

PF12139. APS-reductase_C. 1 hit.
PF00037. Fer4. 1 hit.
[Graphical view]

TIGRFAMs

TIGR02060. aprB. 1 hit.

PROSITE

PS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

O28604.

Entry information

Entry name

O28604_ARCFU

Accession

Primary (citable) accession number: O28604

Entry history

Integrated into UniProtKB/TrEMBL:	January 1, 1998
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)