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Protein
Submitted name:

Adenylylsulfate reductase, subunit A (AprA)

Gene

AF_1670

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Unreviewed-Annotation score: Annotation score: 1 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei74 – 741ADP analogCombined sources
Binding sitei74 – 741SubstrateCombined sources
Binding sitei95 – 951ADP analogCombined sources
Binding sitei95 – 951AMPCombined sources
Binding sitei141 – 1411SubstrateCombined sources
Binding sitei145 – 1451ADP analogCombined sources
Binding sitei145 – 1451AMPCombined sources
Binding sitei214 – 2141FAD; via carbonyl oxygenCombined sources
Binding sitei234 – 2341SubstrateCombined sources
Binding sitei265 – 2651ADP analogCombined sources
Binding sitei265 – 2651FAD cofactor analogCombined sources
Binding sitei265 – 2651SubstrateCombined sources
Binding sitei277 – 2771AMPCombined sources
Binding sitei282 – 2821AMP; via carbonyl oxygenCombined sources
Binding sitei292 – 2921AMPCombined sources
Binding sitei317 – 3171ADP analogCombined sources
Binding sitei317 – 3171AMPCombined sources
Binding sitei361 – 3611SubstrateCombined sources
Binding sitei365 – 3651FADCombined sources
Binding sitei397 – 3971FADCombined sources
Active sitei398 – 3981Proton donor/acceptorCombined sources
Binding sitei439 – 4391FAD cofactor analog; via amide nitrogenCombined sources
Binding sitei439 – 4391FAD; via amide nitrogenCombined sources
Binding sitei449 – 4491FADCombined sources
Binding sitei449 – 4491FAD cofactor analogCombined sources
Sitei449 – 4491Important for catalytic activityCombined sources

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi32 – 332FADCombined sources
Nucleotide bindingi32 – 332FAD cofactor analogCombined sources
Nucleotide bindingi56 – 572FADCombined sources
Nucleotide bindingi56 – 572FAD cofactor analogCombined sources
Nucleotide bindingi63 – 653FADCombined sources
Nucleotide bindingi63 – 653FAD cofactor analogCombined sources
Nucleotide bindingi72 – 743FADCombined sources
Nucleotide bindingi72 – 743FAD cofactor analogCombined sources
Nucleotide bindingi175 – 1762FADCombined sources
Nucleotide bindingi175 – 1762FAD cofactor analogCombined sources
Nucleotide bindingi234 – 2352FADCombined sources
Nucleotide bindingi234 – 2352FAD cofactor analogCombined sources
Nucleotide bindingi239 – 2424FADCombined sources
Nucleotide bindingi239 – 2424FAD cofactor analogCombined sources
Nucleotide bindingi264 – 2652AMPCombined sources
Nucleotide bindingi273 – 2775ADP analogCombined sources
Nucleotide bindingi397 – 3982FAD cofactor analogCombined sources
Nucleotide bindingi398 – 3992ADP analogCombined sources

GO - Molecular functioni

  1. nucleotide binding Source: UniProtKB-KW
  2. oxidoreductase activity Source: InterPro
Complete GO annotation...

Keywords - Ligandi

FADCombined sources, Flavoprotein, Nucleotide-bindingCombined sources

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-1704-MONOMER.
BRENDAi1.8.99.2. 414.

Names & Taxonomyi

Protein namesi
Submitted name:
Adenylylsulfate reductase, subunit A (AprA)Imported
Gene namesi
Ordered Locus Names:AF_1670Imported
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)Imported
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
ProteomesiUP000002199 Componenti: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi224325.AF1670.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JNRX-ray1.60A/C1-643[»]
1JNZX-ray2.50A/C1-643[»]
2FJAX-ray2.00A/C1-643[»]
2FJBX-ray1.70A/C1-643[»]
2FJDX-ray1.84A/C1-643[»]
2FJEX-ray1.80A/C1-643[»]
ProteinModelPortaliO28603.
SMRiO28603. Positions 2-643.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO28603.

Family & Domainsi

Phylogenomic databases

eggNOGiCOG1053.
KOiK00394.
OMAiDAYGPVG.

Family and domain databases

Gene3Di1.20.58.100. 1 hit.
3.90.700.10. 1 hit.
InterProiIPR011803. AprA.
IPR003953. FAD_bind_dom.
IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
IPR027477. Succ_DH/fumarate_Rdtase_cat.
[Graphical view]
PfamiPF00890. FAD_binding_2. 1 hit.
PF02910. Succ_DH_flav_C. 1 hit.
[Graphical view]
SUPFAMiSSF46977. SSF46977. 1 hit.
SSF56425. SSF56425. 1 hit.
TIGRFAMsiTIGR02061. aprA. 1 hit.

Sequencei

Sequence statusi: Complete.

O28603-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVYYPKKYEL YKADEVPTEV VETDILIIGG GFSGCGAAYE AAYWAKLGGL
60 70 80 90 100
KVTLVEKAAV ERSGAVAQGL SAINTYIDLT GRSERQNTLE DYVRYVTLDM
110 120 130 140 150
MGLAREDLVA DYARHVDGTV HLFEKWGLPI WKTPDGKYVR EGQWQIMIHG
160 170 180 190 200
ESYKPIIAEA AKMAVGEENI YERVFIFELL KDKNDPNAVA GAVGFSVREP
210 220 230 240 250
KFYVFKAKAV ILATGGATLL FRPRSTGEAA GRTWYAIFDT GSGYYMGLKA
260 270 280 290 300
GAMLTQFEHR FIPFRFKDGY GPVGAWFLFF KCKAKNAYGE EYIKTRAAEL
310 320 330 340 350
EKYKPYGAAQ PIPTPLRNHQ VMLEIMDGNQ PIYMHTEEAL AELAGGDKKK
360 370 380 390 400
LKHIYEEAFE DFLDMTVSQA LLWACQNIDP QEQPSEAAPA EPYIMGSHSG
410 420 430 440 450
EAGFWVCGPE DLMPEEYAKL FPLKYNRMTT VKGLFAIGDC AGANPHKFSS
460 470 480 490 500
GSFTEGRIAA KAAVRFILEQ KPNPEIDDAV VEELKKKAYA PMERFMQYKD
510 520 530 540 550
LSTADDVNPE YILPWQGLVR LQKIMDEYAA GIATIYKTNE KMLQRALELL
560 570 580 590 600
AFLKEDLEKL AARDLHELMR AWELVHRVWT AEAHVRHMLF RKETRWPGYY
610 620 630 640
YRTDYPELND EEWKCFVCSK YDAEKDEWTF EKVPYVQVIE WSF
Length:643
Mass (Da):73,270
Last modified:December 31, 1997 - v1
Checksum:iA57091382F2E9F4A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB89579.1.
PIRiE69458.
RefSeqiNP_070498.1. NC_000917.1.
WP_010879166.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB89579; AAB89579; AF_1670.
GeneIDi1484893.
KEGGiafu:AF1670.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB89579.1.
PIRiE69458.
RefSeqiNP_070498.1. NC_000917.1.
WP_010879166.1. NC_000917.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JNRX-ray1.60A/C1-643[»]
1JNZX-ray2.50A/C1-643[»]
2FJAX-ray2.00A/C1-643[»]
2FJBX-ray1.70A/C1-643[»]
2FJDX-ray1.84A/C1-643[»]
2FJEX-ray1.80A/C1-643[»]
ProteinModelPortaliO28603.
SMRiO28603. Positions 2-643.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224325.AF1670.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB89579; AAB89579; AF_1670.
GeneIDi1484893.
KEGGiafu:AF1670.

Phylogenomic databases

eggNOGiCOG1053.
KOiK00394.
OMAiDAYGPVG.

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-1704-MONOMER.
BRENDAi1.8.99.2. 414.

Miscellaneous databases

EvolutionaryTraceiO28603.

Family and domain databases

Gene3Di1.20.58.100. 1 hit.
3.90.700.10. 1 hit.
InterProiIPR011803. AprA.
IPR003953. FAD_bind_dom.
IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
IPR027477. Succ_DH/fumarate_Rdtase_cat.
[Graphical view]
PfamiPF00890. FAD_binding_2. 1 hit.
PF02910. Succ_DH_flav_C. 1 hit.
[Graphical view]
SUPFAMiSSF46977. SSF46977. 1 hit.
SSF56425. SSF56425. 1 hit.
TIGRFAMsiTIGR02061. aprA. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus."
    Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G.
    , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C.
    Nature 390:364-370(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126Imported.
  2. "Structure of adenylylsulfate reductase from the hyperthermophilic Archaeoglobus fulgidus at 1.6-A resolution."
    Fritz G., Roth A., Schiffer A., Buchert T., Bourenkov G., Bartunik H.D., Huber H., Stetter K.O., Kroneck P.M., Ermler U.
    Proc. Natl. Acad. Sci. U.S.A. 99:1836-1841(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) IN COMPLEX WITH FAD, ACTIVE SITE.
  3. "Reaction mechanism of the iron-sulfur flavoenzyme adenosine-5'-phosphosulfate reductase based on the structural characterization of different enzymatic states."
    Schiffer A., Fritz G., Kroneck P.M., Ermler U.
    Biochemistry 45:2960-2967(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) IN COMPLEX WITH ADP ANALOG; AMP; FAD AND FAD COFACTOR ANALOG.

Entry informationi

Entry nameiO28603_ARCFU
AccessioniPrimary (citable) accession number: O28603
Entry historyi
Integrated into UniProtKB/TrEMBL: December 31, 1997
Last sequence update: December 31, 1997
Last modified: March 31, 2015
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.