Reviewed, UniProtKB/Swiss-Prot O28578 (PANE_ARCFU)

Names and origin

Protein names

Recommended name:
    Putative 2-dehydropantoate 2-reductase
    EC=1.1.1.169
Alternative name(s):
    Ketopantoate reductase
      Short name=KPA reductase
      Short name=KPR

Gene names

Ordered Locus Names:

AF_1695

Organism

Archaeoglobus fulgidus [Complete proteome] [HAMAP]

Taxonomic identifier

2234 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid By similarity.
Catalytic activity	(R)-pantoate + NADP+ = 2-dehydropantoate + NADPH.
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 2/2.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the ketopantoate reductase family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pantothenate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	2-dehydropantoate 2-reductase activity Inferred from electronic annotation. Source: EC NADP or NADPH binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 294	294	Putative 2-dehydropantoate 2-reductase		PRO_0000157322
Regions
	Nucleotide binding	10 – 15	6	NADP By similarity
Sites
	Active site	178	1	Proton donor By similarity
	Binding site	98	1	NADP; via amide nitrogen By similarity
	Binding site	98	1	Substrate By similarity
	Binding site	182	1	Substrate By similarity
	Binding site	186	1	Substrate By similarity
	Binding site	246	1	Substrate By similarity
	Binding site	258	1	NADP By similarity

Sequences

	Sequence		Length	Mass (Da)
	O28578-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: A0AFE175763A4184	FASTA	294	31,885
	10 20 30 40 50 60 MRVQRVQIMG AGALGSLVGA LIQLAGYDVI FVARGKQLEA LKKGLRVSGL KNAELKVYCT 70 80 90 100 110 120 SQPEDADITF VTVKAYDTET VAKKLAEVDA GVVCSLQNGV GNEEILAKYC RKVLGGVTTY 130 140 150 160 170 180 GANLKDYGHV VYAGEGYTYV GEMDGRVSGE AEMVAEVLRD AGMRAEAVND IEFRIWAKAV 190 200 210 220 230 240 VNAAINPITA ICRVKNGEVV RNPHLWEVAR AVADEGRQVM ARMGYEFDAA SEVRKVAEMT 250 260 270 280 290 AENRSSMLQD LERGKRTEVE FINGAIVKKG EEFGIDCAVN RTLLNLVRGV ESGL

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000782 Genomic DNA. Translation: AAB89553.1.
PIR	F69461.
RefSeq	NP_070523.1.
3D structure databases
HSSP	HSSP built from PDB template 1KS9 based on UniProtKB P77728.
ModBase	Search...
Genome annotation databases
GeneID	1484918.
GenomeReviews	Gene locus AF_1695 in contig AE000782_GR.
KEGG	afu:AF1695.
NMPDR	fig|224325.1.peg.1684.
TIGR	AF_1695.
Phylogenomic databases
HOGENOM	O28578.
OMA	DIAREGC.
Enzyme and pathway databases
BioCyc	AFUL224325:AF_1695-MON.
BRENDA	1.1.1.169. 7576.
Family and domain databases
InterPro	IPR003710. ApbA. IPR013752. ApbA_C. IPR013332. ApbA_N. IPR013328. DH_multihelical. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
PANTHER	PTHR21708:SF21. ApbA. 1 hit.
Pfam	PF02558. ApbA. 1 hit. PF08546. ApbA_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00745. apbA_panE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANE_ARCFU

Accession

Primary (citable) accession number: O28578

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	November 3, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)