Reviewed, UniProtKB/Swiss-Prot O28552 (DP2L_ARCFU)

Names and origin

Protein names

Recommended name:
    DNA polymerase II large subunit
      Short name=Pol II
    EC=2.7.7.7

Gene names

Name:	polC
Ordered Locus Names:	AF_1722

Organism

Archaeoglobus fulgidus [Complete proteome] [HAMAP]

Taxonomic identifier

2234 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	1143 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_00324 Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates. HAMAP MF_00324
Subunit structure	Heterodimer of a large subunit and a small subunit By similarity.
Sequence similarities	Belongs to the archaeal DNA polymerase II family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Exonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	DNA catabolic process, exonucleolytic Inferred from electronic annotation. Source: HAMAP DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: HAMAP DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1143	1143	DNA polymerase II large subunit HAMAP MF_00324		PRO_0000152573

Sequences

	Sequence		Length	Mass (Da)
	O28552-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 7D874532600D851F	FASTA	1,143	129,368
	10 20 30 40 50 60 MDATLDRFFP LFESESNEDF WRIEEIRRYH ESLMVELDRI YRIAEAARKK GLDPELSVEI 70 80 90 100 110 120 PIAKNMAERV EKLMNLQGLA KRIMELEEGG LSRELICFKV ADEIVEGKFG EMPKEEAIDK 130 140 150 160 170 180 AVRTAVAIMT EGVVAAPIEG IARVRIDREN FLRVYYAGPI RSAGGTAQVI SVLVADYVRR 190 200 210 220 230 240 KAEIGRYVPT EEEILRYCEE IPLYKKVANL QYLPSDEEIR LIVSNCPICI DGEPTESAEV 250 260 270 280 290 300 SGYRNLPRVE TNRVRGGMAL VIAEGIALKA PKLKKMVDEV GIEGWEWLDA LIKGGGDSGS 310 320 330 340 350 360 EEEKAVIKPK DKYLSDIVAG RPVLSHPSRK GGFRLRYGRA RNSGFATVGV NPATMYLLEF 370 380 390 400 410 420 VAVGTQLKVE RPGKAGGVVP VSTIEGPTVR LKNGDVVKIN TLSEAKALKG EVAAILDLGE 430 440 450 460 470 480 ILINYGDFLE NNHPLIPASY TYEWWIQEAE KAGLRGDYRK ISEEEALKLC DEFHVPLHPD 490 500 510 520 530 540 YTYLWHDISV EDYRYLRNFV SDNGKIEGKH GKSVLLLPYD SRVKEILEAL LLEHKVRESF 550 560 570 580 590 600 IVIETWRAFI RCLGLDEKLS KVSEVSGKDV LEIVNGISGI KVRPKALSRI GARMGRPEKA 610 620 630 640 650 660 KERKMSPPPH ILFPVGMAGG NTRDIKNAIN YTKSYNAKKG EIEVEIAIRK CPQCGKETFW 670 680 690 700 710 720 LKCDVCGELT EQLYYCPSCR MKNTSSVCES CGRECEGYMK RKVDLRELYE EAIANLGEYD 730 740 750 760 770 780 SFDTIKGVKG MTSKTKIPER LEKGILRVKH GVFVFKDGTA RFDATDLPIT HFKPAEIGVS 790 800 810 820 830 840 VEKLRELGYE RDYKGAELKN ENQIVELKPQ DVILPKSGAE YLLRVANFID DLLVKFYKME 850 860 870 880 890 900 PFYNAKSVED LIGHLVIGLA PHTSAGVLGR IIGFSDVLAG YAHPYFHAAK RRNCDGDEDC 910 920 930 940 950 960 FMLLLDGLLN FSRKFLPDKR GGQMDAPLVL TAIVDPREVD KEVHNMDIVE RYPLEFYEAT 970 980 990 1000 1010 1020 MRFASPKEME DYVEKVKDRL KDESRFCGLF FTHDTENIAA GVKESAYKSL KTMQDKVYRQ 1030 1040 1050 1060 1070 1080 MELARMIVAV DEHDVAERVI NVHFLPDIIG NLRAFSRQEF RCTRCNTKYR RIPLVGKCLK 1090 1100 1110 1120 1130 1140 CGNKLTLTVH SSSIMKYLEL SKFLCENFNV SSYTKQRLML LEQEIKSMFE NGTEKQVSIS DFV

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000782 Genomic DNA. Translation: AAB89529.1.
PIR	A69465.
RefSeq	NP_070550.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1484945.
GenomeReviews	Gene locus AF_1722 in contig AE000782_GR.
KEGG	afu:AF1722.
NMPDR	fig|224325.1.peg.1711.
TIGR	AF_1722.
Phylogenomic databases
HOGENOM	O28552.
OMA	NCDGDED.
Enzyme and pathway databases
BioCyc	AFUL224325:AF_1722-MON.
BRENDA	2.7.7.7. 7576.
Family and domain databases
HAMAP	MF_00324. [Tree]
InterPro	IPR004475. PolC_DP2. IPR016033. PolC_DP2_N. [Graphical view]
Pfam	PF03833. PolC_DP2. 1 hit. [Graphical view]
PIRSF	PIRSF016275. PolC_DP2. 1 hit.
TIGRFAMs	TIGR00354. polC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DP2L_ARCFU

Accession

Primary (citable) accession number: O28552

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	January 1, 1998
Last modified:	November 3, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)