ID   G3P_ARCFU               Reviewed;         340 AA.
AC   O28542;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   16-JUN-2009, entry version 68.
DE   RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase;
DE            Short=GAPDH;
DE            EC=1.2.1.59;
DE   AltName: Full=NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase;
GN   Name=gap; OrderedLocusNames=AF_1732;
OS   Archaeoglobus fulgidus.
OC   Archaea; Euryarchaeota; Archaeoglobi; Archaeoglobales;
OC   Archaeoglobaceae; Archaeoglobus.
OX   NCBI_TaxID=2234;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126;
RX   MEDLINE=98049343; PubMed=9389475; DOI=10.1038/37052;
RA   Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E.,
RA   Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D.,
RA   Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C.,
RA   Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G., Gill S.R.,
RA   Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J.,
RA   Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A.,
RA   Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A.,
RA   Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P.,
RA   Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C.,
RA   Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O.,
RA   Woese C.R., Venter J.C.;
RT   "The complete genome sequence of the hyperthermophilic, sulphate-
RT   reducing archaeon Archaeoglobus fulgidus.";
RL   Nature 390:364-370(1997).
CC   -!- CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate +
CC       NAD(P)(+) = 3-phospho-D-glyceroyl phosphate + NAD(P)H.
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-
CC       glyceraldehyde 3-phosphate: step 1/5.
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the glyceraldehyde-3-phosphate
CC       dehydrogenase family.
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DR   EMBL; AE000782; AAB89515.1; -; Genomic_DNA.
DR   PIR; C69466; C69466.
DR   RefSeq; NP_070560.1; -.
DR   HSSP; P10618; 1CF2.
DR   GeneID; 1484955; -.
DR   GenomeReviews; AE000782_GR; AF_1732.
DR   KEGG; afu:AF1732; -.
DR   NMPDR; fig|224325.1.peg.1721; -.
DR   TIGR; AF_1732; -.
DR   HOGENOM; O28542; -.
DR   OMA; O28542; AIFQGGE.
DR   BioCyc; AFUL224325:AF_1732-MON; -.
DR   BRENDA; 1.2.1.59; 7576.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0043891; F:glyceraldehyde-3-phosphate dehydrogenase (N...; IEA:EC.
DR   GO; GO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (p...; IEA:HAMAP.
DR   GO; GO:0051287; F:NAD or NADH binding; IEA:InterPro.
DR   GO; GO:0050661; F:NADP or NADPH binding; IEA:InterPro.
DR   GO; GO:0006096; P:glycolysis; IEA:HAMAP.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_00559; -; 1.
DR   InterPro; IPR000173; GlycerAld_3-P_DH.
DR   InterPro; IPR006436; Glyceraldehyde-3-P_DH_2_arc.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR10836; GAP_DH; 1.
DR   Pfam; PF02800; Gp_dh_C; 1.
DR   Pfam; PF00044; Gp_dh_N; 1.
DR   PIRSF; PIRSF000149; GAP_DH; 1.
DR   ProDom; PD007761; GAPDH_like; 1.
DR   TIGRFAMs; TIGR01546; GAPDH-II_archae; 1.
DR   PROSITE; PS00071; GAPDH; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; Glycolysis; NAD; NADP; Oxidoreductase.
FT   CHAIN         1    340       Glyceraldehyde-3-phosphate dehydrogenase.
FT                                /FTId=PRO_0000145716.
FT   NP_BIND      13     14       NAD (By similarity).
FT   REGION      141    143       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   REGION      196    197       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   ACT_SITE    142    142       Nucleophile (By similarity).
FT   BINDING     112    112       NAD; via amide nitrogen (By similarity).
FT   BINDING     170    170       NAD (By similarity).
FT   BINDING     302    302       NAD; via carbonyl oxygen (By similarity).
SQ   SEQUENCE   340 AA;  37619 MW;  133DF2C3765E20E0 CRC64;
     MMKVKVAING YGTIGKRVAD AVSLQDDMEV VGVTKTRPDF EAKLGAKRYP LYVAKPENVE
     LFERAGIEIQ GTIEDLLPKA DIVVDCSPNK VGAENKAKYY EKAGIKAIFQ GGEKKDVAEV
     SFNALANYDE AVGKSYVRVV SCNTTGLTRL IYMLKTNFSI GRIRATMLRR VVDPKEDKKG
     LVNGIMPDPV AIPSHHGPDV KTVLPDVDIV TTAFKLPTTL MHVHSLCVEM REAVKAEDVV
     SALSEEPRIM LISAEDGFTS TAKVIEFARE LRLRYDLYEN IVWRESIGVD GNDLFVTQAV
     HQEAIVVPEN IDAIRAMFEL AEKEESIRKT NESLGIGKVF
//