Reviewed, UniProtKB/Swiss-Prot O28542 (G3P_ARCFU)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase
      Short name=GAPDH
    EC=1.2.1.59
Alternative name(s):
    NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase

Gene names

Name:	gap
Ordered Locus Names:	AF_1732

Organism

Archaeoglobus fulgidus [Complete proteome] [HAMAP]

Taxonomic identifier

2234 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H. HAMAP MF_00559
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. HAMAP MF_00559
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NAD(P)+) (phosphorylating) activity Inferred from electronic annotation. Source: EC glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	Glyceraldehyde-3-phosphate dehydrogenase HAMAP MF_00559		PRO_0000145716
Regions
	Nucleotide binding	13 – 14	2	NAD By similarity
	Region	141 – 143	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	196 – 197	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	142	1	Nucleophile By similarity
	Binding site	112	1	NAD; via amide nitrogen By similarity
	Binding site	170	1	NAD By similarity
	Binding site	302	1	NAD; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	O28542-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 133DF2C3765E20E0	FASTA	340	37,619
	10 20 30 40 50 60 MMKVKVAING YGTIGKRVAD AVSLQDDMEV VGVTKTRPDF EAKLGAKRYP LYVAKPENVE 70 80 90 100 110 120 LFERAGIEIQ GTIEDLLPKA DIVVDCSPNK VGAENKAKYY EKAGIKAIFQ GGEKKDVAEV 130 140 150 160 170 180 SFNALANYDE AVGKSYVRVV SCNTTGLTRL IYMLKTNFSI GRIRATMLRR VVDPKEDKKG 190 200 210 220 230 240 LVNGIMPDPV AIPSHHGPDV KTVLPDVDIV TTAFKLPTTL MHVHSLCVEM REAVKAEDVV 250 260 270 280 290 300 SALSEEPRIM LISAEDGFTS TAKVIEFARE LRLRYDLYEN IVWRESIGVD GNDLFVTQAV 310 320 330 340 HQEAIVVPEN IDAIRAMFEL AEKEESIRKT NESLGIGKVF

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000782 Genomic DNA. Translation: AAB89515.1.
PIR	C69466.
RefSeq	NP_070560.1.
3D structure databases
HSSP	HSSP built from PDB template 1CF2 based on UniProtKB P10618.
ModBase	Search...
Genome annotation databases
GeneID	1484955.
GenomeReviews	Gene locus AF_1732 in contig AE000782_GR.
KEGG	afu:AF1732.
NMPDR	fig|224325.1.peg.1721.
TIGR	AF_1732.
Phylogenomic databases
HOGENOM	O28542.
OMA	O28542. AIFQGGE.
Enzyme and pathway databases
BioCyc	AFUL224325:AF_1732-MON.
BRENDA	1.2.1.59. 7576.
Family and domain databases
HAMAP	MF_00559. [Tree]
InterPro	IPR000173. GlycerAld_3-P_DH. IPR006436. Glyceraldehyde-3-P_DH_2_arc. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
ProDom	PD007761. GAPDH_like. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01546. GAPDH-II_archae. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3P_ARCFU

Accession

Primary (citable) accession number: O28542

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)