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Protein

Glyceraldehyde-3-phosphate dehydrogenase

Gene

gap

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H.

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gap)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 1 (apgM1), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 2 (apgM2)
  4. Enolase (eno)
  5. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei112NAD; via amide nitrogenBy similarity1
Active sitei142NucleophileBy similarity1
Binding sitei170NADBy similarity1
Binding sitei302NAD; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 14NADBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processGlycolysis
LigandNAD, NADP

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase (EC:1.2.1.59)
Short name:
GAPDH
Alternative name(s):
NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase
Gene namesi
Name:gap
Ordered Locus Names:AF_1732
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001457161 – 340Glyceraldehyde-3-phosphate dehydrogenaseAdd BLAST340

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi224325.AF1732.

Structurei

3D structure databases

ProteinModelPortaliO28542.
SMRiO28542.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni141 – 143Glyceraldehyde 3-phosphate bindingBy similarity3
Regioni196 – 197Glyceraldehyde 3-phosphate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG00493. Archaea.
COG0057. LUCA.
KOiK00150.
OMAiNAIVPNP.
OrthoDBiPOG093Z05Z6.

Family and domain databases

HAMAPiMF_00559. G3P_dehdrog_arch. 1 hit.
InterProiView protein in InterPro
IPR000846. DapB_N.
IPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006436. Glyceraldehyde-3-P_DH_2_arc.
IPR036291. NAD(P)-bd_dom_sf.
PANTHERiPTHR10836. PTHR10836. 1 hit.
PTHR10836:SF76. PTHR10836:SF76. 1 hit.
PfamiView protein in Pfam
PF01113. DapB_N. 1 hit.
PF02800. Gp_dh_C. 1 hit.
PIRSFiPIRSF000149. GAP_DH. 1 hit.
SMARTiView protein in SMART
SM00846. Gp_dh_N. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01546. GAPDH-II_archae. 1 hit.
PROSITEiView protein in PROSITE
PS00071. GAPDH. 1 hit.

Sequencei

Sequence statusi: Complete.

O28542-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMKVKVAING YGTIGKRVAD AVSLQDDMEV VGVTKTRPDF EAKLGAKRYP
60 70 80 90 100
LYVAKPENVE LFERAGIEIQ GTIEDLLPKA DIVVDCSPNK VGAENKAKYY
110 120 130 140 150
EKAGIKAIFQ GGEKKDVAEV SFNALANYDE AVGKSYVRVV SCNTTGLTRL
160 170 180 190 200
IYMLKTNFSI GRIRATMLRR VVDPKEDKKG LVNGIMPDPV AIPSHHGPDV
210 220 230 240 250
KTVLPDVDIV TTAFKLPTTL MHVHSLCVEM REAVKAEDVV SALSEEPRIM
260 270 280 290 300
LISAEDGFTS TAKVIEFARE LRLRYDLYEN IVWRESIGVD GNDLFVTQAV
310 320 330 340
HQEAIVVPEN IDAIRAMFEL AEKEESIRKT NESLGIGKVF
Length:340
Mass (Da):37,619
Last modified:January 1, 1998 - v1
Checksum:i133DF2C3765E20E0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB89515.1.
PIRiC69466.

Genome annotation databases

EnsemblBacteriaiAAB89515; AAB89515; AF_1732.
KEGGiafu:AF_1732.

Similar proteinsi

Entry informationi

Entry nameiG3P_ARCFU
AccessioniPrimary (citable) accession number: O28542
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: November 22, 2017
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families