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O28542 (G3P_ARCFU) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glyceraldehyde-3-phosphate dehydrogenase
Short name=GAPDH
EC=1.2.1.59
Alternative name(s):
NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase
Gene names
Name:gap
Ordered Locus Names:AF_1732
OrganismArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifier224325 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length340 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H. HAMAP MF_00559

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. HAMAP MF_00559

Subunit structure

Homotetramer By similarity. HAMAP MF_00559

Subcellular location

Cytoplasm By similarity HAMAP MF_00559.

Sequence similarities

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandNAD
NADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processglycolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionNAD binding

Inferred from electronic annotation. Source: InterPro

NADP binding

Inferred from electronic annotation. Source: InterPro

glyceraldehyde-3-phosphate dehydrogenase (NAD(P)+) (phosphorylating) activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 340340Glyceraldehyde-3-phosphate dehydrogenase HAMAP MF_00559
PRO_0000145716

Regions

Nucleotide binding13 – 142NAD By similarity
Region141 – 1433Glyceraldehyde 3-phosphate binding By similarity
Region196 – 1972Glyceraldehyde 3-phosphate binding By similarity

Sites

Active site1421Nucleophile By similarity
Binding site1121NAD; via amide nitrogen By similarity
Binding site1701NAD By similarity
Binding site3021NAD; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
O28542 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 133DF2C3765E20E0

FASTA34037,619
        10         20         30         40         50         60 
MMKVKVAING YGTIGKRVAD AVSLQDDMEV VGVTKTRPDF EAKLGAKRYP LYVAKPENVE 

        70         80         90        100        110        120 
LFERAGIEIQ GTIEDLLPKA DIVVDCSPNK VGAENKAKYY EKAGIKAIFQ GGEKKDVAEV 

       130        140        150        160        170        180 
SFNALANYDE AVGKSYVRVV SCNTTGLTRL IYMLKTNFSI GRIRATMLRR VVDPKEDKKG 

       190        200        210        220        230        240 
LVNGIMPDPV AIPSHHGPDV KTVLPDVDIV TTAFKLPTTL MHVHSLCVEM REAVKAEDVV 

       250        260        270        280        290        300 
SALSEEPRIM LISAEDGFTS TAKVIEFARE LRLRYDLYEN IVWRESIGVD GNDLFVTQAV 

       310        320        330        340 
HQEAIVVPEN IDAIRAMFEL AEKEESIRKT NESLGIGKVF

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE000782 Genomic DNA. Translation: AAB89515.1.
PIRC69466.
RefSeqNP_070560.1. NC_000917.1.

3D structure databases

ProteinModelPortalO28542.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1484955.
GenomeReviewsGene locus AF_1732 in contig AE000782_GR.
KEGGafu:AF1732.
NMPDRfig|224325.1.peg.1721.
TIGRAF_1732.

Phylogenomic databases

HOGENOMHBG392099.
OMAVPSHHGP.
ProtClustDBPRK04207.

Enzyme and pathway databases

BioCycAFUL224325:AF_1732-MONOMER.

Family and domain databases

HAMAPMF_00559. G3P_dehdrog_arch.
[Tree]
InterProIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006436. Glyceraldehyde-3-P_DH_2_arc.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KOK00150.
PfamPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFPIRSF000149. GAP_DH. 1 hit.
SMARTSM00846. Gp_dh_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR01546. GAPDH-II_archae. 1 hit.
PROSITEPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG3P_ARCFU
AccessionPrimary (citable) accession number: O28542
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: November 16, 2011
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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