Reviewed, UniProtKB/Swiss-Prot O28533 (PYRE_ARCFU)

Names and origin

Protein names

Recommended name:
    Orotate phosphoribosyltransferase
      Short name=OPRT
      Short name=OPRTase
    EC=2.4.2.10

Gene names

Name:	pyrE
Ordered Locus Names:	AF_1741

Organism

Archaeoglobus fulgidus [Complete proteome] [HAMAP]

Taxonomic identifier

2234 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	178 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. HAMAP MF_01208
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208
Cofactor	Magnesium By similarity. HAMAP MF_01208
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208
Subunit structure	Homodimer By similarity. HAMAP MF_01208
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Magnesium
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 178	178	Orotate phosphoribosyltransferase HAMAP MF_01208		PRO_0000110775
Regions
	Region	118 – 126	9	5-phosphoribose 1-diphosphate binding By similarity
Sites
	Binding site	92	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	93	1	5-phosphoribose 1-diphosphate By similarity
	Binding site	96	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	122	1	Orotate By similarity
	Binding site	150	1	Orotate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O28533-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 46D80741CC74FF19	FASTA	178	19,301
	10 20 30 40 50 60 MVMLADALVK RMIEVGALKF GDFVLSSGKR SRVYVDVKLA STFPDILEMI SEGMAAKLKD 70 80 90 100 110 120 LEFDRIACVE LGGVPIAVAL SLKMKKPLVI FRKEKKDYGI KGDRIGEVKE GEKVVVVEDV 130 140 150 160 170 ITTGSSALSA ARRVEESGAS VAAIIAVVDR EESGRNFMSL LKLSDLIEAH DSIQPTES

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000782 Genomic DNA. Translation: AAB89504.1.
PIR	D69467.
RefSeq	NP_070569.1.
3D structure databases
SMR	O28533. Positions 4-170.
ModBase	Search...
Genome annotation databases
GeneID	1484964.
GenomeReviews	Gene locus AF_1741 in contig AE000782_GR.
KEGG	afu:AF1741.
NMPDR	fig|224325.1.peg.1730.
TIGR	AF_1741.
Phylogenomic databases
HOGENOM	HBG404341.
OMA	LSYPRIR.
Enzyme and pathway databases
BioCyc	AFUL224325:AF_1741-MONOMER.
BRENDA	2.4.2.10. 7576.
Family and domain databases
HAMAP	MF_01208_A. PyrE_A. [Tree]
InterPro	IPR004467. Or_phspho_trans. IPR000836. PRibTrfase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR00336. pyrE. 1 hit.
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRE_ARCFU

Accession

Primary (citable) accession number: O28533

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	February 9, 2010
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)