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O28486 (PNPH_ARCFU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable 6-oxopurine nucleoside phosphorylase

EC=2.4.2.1
Alternative name(s):
Purine nucleoside phosphorylase
Short name=PNP
Gene names
Ordered Locus Names:AF_1788
OrganismArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) [Reference proteome] [HAMAP]
Taxonomic identifier224325 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus

Protein attributes

Sequence length243 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Purine nucleoside phosphorylase which is highly specific for 6-oxopurine nucleosides. Cleaves guanosine or inosine to respective bases and sugar-1-phosphate molecules. Involved in purine salvage By similarity. HAMAP-Rule MF_01963

Catalytic activity

Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate. HAMAP-Rule MF_01963

Pathway

Purine metabolism; purine nucleoside salvage. HAMAP-Rule MF_01963

Subunit structure

Homohexamer. Dimer of a homotrimer By similarity. HAMAP-Rule MF_01963

Miscellaneous

Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it has been shown that conserved amino acid substitutions in the substrate binding pocket convert the substrate specificity of this enzyme from 6-aminopurines to 6-oxopurines By similarity.

Sequence similarities

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 243243Probable 6-oxopurine nucleoside phosphorylase HAMAP-Rule MF_01963
PRO_0000415084

Regions

Region48 – 492Phosphate binding By similarity
Region198 – 2003Substrate binding By similarity

Sites

Binding site81Phosphate By similarity
Binding site1741Substrate; via amide nitrogen By similarity
Binding site1751Phosphate By similarity
Site1571Important for substrate specificity By similarity
Site2101Important for substrate specificity By similarity

Sequences

Sequence LengthMass (Da)Tools
O28486 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: C8FCFE74DAC89DCC

FASTA24327,201
        10         20         30         40         50         60 
MIGIIGGTHI LEIKVLKDVE ETRIETPYGT AEIDVGRVDG IDVAIIQRHG KRKDKPPHRI 

        70         80         90        100        110        120 
NHAANFYALK SLGVKYVIGM GSVGALREEY SLPSLIIPHD YIDFFSGVTI YNDSLVHVTP 

       130        140        150        160        170        180 
GFDEYLREVL VEVARKISSF PVIDKGVYFQ TRGPRLETKA EIAMIKSFAD CVGMTAGSEA 

       190        200        210        220        230        240 
TIARELGLSY AIVCTMDNYA HGIKNQSIDY REIVEKAKEN ARECLKIVEE AVKKVWEEKI 


QRT 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000782 Genomic DNA. Translation: AAB89459.1.
PIRC69473.
RefSeqNP_070616.1. NC_000917.1.

3D structure databases

ProteinModelPortalO28486.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224325.AF1788.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB89459; AAB89459; AF_1788.
GeneID1485011.
KEGGafu:AF1788.

Phylogenomic databases

eggNOGCOG0005.
KOK00772.
OMACTPFGKP.

Enzyme and pathway databases

BioCycAFUL224325:GJBC-1823-MONOMER.
UniPathwayUPA00606.

Family and domain databases

Gene3D3.40.50.1580. 1 hit.
HAMAPMF_01963. MTAP.
InterProIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
[Graphical view]
PANTHERPTHR11904. PTHR11904. 1 hit.
PfamPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMSSF53167. SSF53167. 1 hit.
TIGRFAMsTIGR01694. MTAP. 1 hit.
ProtoNetSearch...

Entry information

Entry namePNPH_ARCFU
AccessionPrimary (citable) accession number: O28486
Entry history
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: January 1, 1998
Last modified: April 16, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways