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Protein

RIO-type serine/threonine-protein kinase Rio1

Gene

rio1

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Autophosphorylation of the rio1 protein is not necessary for maintenance of kinase activity. Prefers ATP over GTP. The yeast ortholog is involved in ribosome biogenesis.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei80 – 801ATP
Binding sitei148 – 1481ATP; via carbonyl oxygen
Binding sitei150 – 1501ATP; via amide nitrogen
Active sitei196 – 1961Proton acceptorPROSITE-ProRule annotation
Metal bindingi201 – 2011Magnesium
Binding sitei201 – 2011ATP
Metal bindingi212 – 2121Magnesium
Binding sitei212 – 2121ATP

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi55 – 639ATPPROSITE-ProRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-1839-MONOMER.
BRENDAi2.7.11.1. 414.

Names & Taxonomyi

Protein namesi
Recommended name:
RIO-type serine/threonine-protein kinase Rio1 (EC:2.7.11.1)
Short name:
AfRio1
Gene namesi
Name:rio1
Ordered Locus Names:AF_1804
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
ProteomesiUP000002199 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi108 – 1081S → A: Absence of autophosphorylation, retains kinase activity. 1 Publication
Mutagenesisi196 – 1961D → A: Drastically reduced kinase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 258258RIO-type serine/threonine-protein kinase Rio1PRO_0000344796Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei108 – 1081Phosphoserine; by autocatalysis1 Publication

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi224325.AF1804.

Structurei

Secondary structure

1
258
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 1310Combined sources
Helixi18 – 203Combined sources
Helixi23 – 308Combined sources
Helixi33 – 4412Combined sources
Beta strandi47 – 5711Combined sources
Beta strandi59 – 7012Combined sources
Beta strandi73 – 8210Combined sources
Helixi93 – 953Combined sources
Turni96 – 983Combined sources
Helixi104 – 1063Combined sources
Helixi110 – 12920Combined sources
Beta strandi137 – 1415Combined sources
Beta strandi144 – 1485Combined sources
Helixi160 – 1634Combined sources
Helixi164 – 1696Combined sources
Helixi172 – 18817Combined sources
Beta strandi192 – 1943Combined sources
Beta strandi201 – 21010Combined sources
Helixi213 – 2153Combined sources
Beta strandi216 – 2183Combined sources
Helixi224 – 24017Combined sources
Turni241 – 2433Combined sources
Helixi248 – 2569Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZP9X-ray2.00A/B/C/D1-258[»]
1ZTFX-ray1.99A1-258[»]
1ZTHX-ray1.89A/B/C/D1-258[»]
3RE4X-ray2.00A/B1-258[»]
4JINX-ray2.10A1-258[»]
ProteinModelPortaliO28471.
SMRiO28471. Positions 5-257.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO28471.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini49 – 258210Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG1718.
KOiK07178.
OMAiGDPRFHL.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR018934. RIO_dom.
IPR000687. RIO_kinase.
IPR018935. RIO_kinase_CS.
IPR017407. Ser/Thr_kinase_Rio1.
[Graphical view]
PANTHERiPTHR10593:SF23. PTHR10593:SF23. 1 hit.
PfamiPF01163. RIO1. 1 hit.
[Graphical view]
SMARTiSM00090. RIO. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS01245. RIO1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O28471-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDLKKIESY LDKLRIKEKD GEERKIYAEV LDGRTLKTLY KLSAKGYITA
60 70 80 90 100
MGGVISTGKE ANVFYADGVF DGKPVAMAVK IYRIETSEFD KMDEYLYGDE
110 120 130 140 150
RFDMRRISPK EKVFIWTEKE FRNLERAKEA GVSVPQPYTY MKNVLLMEFI
160 170 180 190 200
GEDELPAPTL VELGRELKEL DVEGIFNDVV ENVKRLYQEA ELVHADLSEY
210 220 230 240 250
NIMYIDKVYF IDMGQAVTLR HPMAESYLER DVRNIIRFFS KYGVKADFEE

MLKEVKGE
Length:258
Mass (Da):30,095
Last modified:January 1, 1998 - v1
Checksum:i70CA739CA39A4863
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB89445.1.
PIRiC69475.
RefSeqiNP_070631.1. NC_000917.1.
WP_010879299.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB89445; AAB89445; AF_1804.
GeneIDi1485027.
KEGGiafu:AF1804.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB89445.1.
PIRiC69475.
RefSeqiNP_070631.1. NC_000917.1.
WP_010879299.1. NC_000917.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZP9X-ray2.00A/B/C/D1-258[»]
1ZTFX-ray1.99A1-258[»]
1ZTHX-ray1.89A/B/C/D1-258[»]
3RE4X-ray2.00A/B1-258[»]
4JINX-ray2.10A1-258[»]
ProteinModelPortaliO28471.
SMRiO28471. Positions 5-257.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224325.AF1804.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB89445; AAB89445; AF_1804.
GeneIDi1485027.
KEGGiafu:AF1804.

Phylogenomic databases

eggNOGiCOG1718.
KOiK07178.
OMAiGDPRFHL.

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-1839-MONOMER.
BRENDAi2.7.11.1. 414.

Miscellaneous databases

EvolutionaryTraceiO28471.
PROiO28471.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR018934. RIO_dom.
IPR000687. RIO_kinase.
IPR018935. RIO_kinase_CS.
IPR017407. Ser/Thr_kinase_Rio1.
[Graphical view]
PANTHERiPTHR10593:SF23. PTHR10593:SF23. 1 hit.
PfamiPF01163. RIO1. 1 hit.
[Graphical view]
SMARTiSM00090. RIO. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS01245. RIO1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus."
    Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G.
    , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C.
    Nature 390:364-370(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.
  2. "Structure and activity of the atypical serine kinase Rio1."
    Laronde-Leblanc N., Guszczynski T., Copeland T., Wlodawer A.
    FEBS J. 272:3698-3713(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 99-110, X-RAY CRYSTALLOGRAPHY (1.89 ANGSTROMS) IN PRESENCE AND ABSENCE OF ATP, PHOSPHORYLATION AT SER-108, MUTAGENESIS OF SER-108 AND ASP-196.
    Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

Entry informationi

Entry nameiRIO1_ARCFU
AccessioniPrimary (citable) accession number: O28471
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: January 1, 1998
Last modified: May 27, 2015
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.