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O28471

- RIO1_ARCFU

UniProt

O28471 - RIO1_ARCFU

Protein

RIO-type serine/threonine-protein kinase Rio1

Gene

rio1

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    • Comment

    Functioni

    Autophosphorylation of the rio1 protein is not necessary for maintenance of kinase activity. Prefers ATP over GTP. The yeast ortholog is involved in ribosome biogenesis.

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Magnesium.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei80 – 801ATP
    Binding sitei148 – 1481ATP; via carbonyl oxygen
    Binding sitei150 – 1501ATP; via amide nitrogen
    Active sitei196 – 1961Proton acceptorPROSITE-ProRule annotation
    Metal bindingi201 – 2011Magnesium
    Binding sitei201 – 2011ATP
    Metal bindingi212 – 2121Magnesium
    Binding sitei212 – 2121ATP

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi55 – 639ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. protein serine/threonine kinase activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciAFUL224325:GJBC-1839-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RIO-type serine/threonine-protein kinase Rio1 (EC:2.7.11.1)
    Short name:
    AfRio1
    Gene namesi
    Name:rio1
    Ordered Locus Names:AF_1804
    OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
    Taxonomic identifieri224325 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
    ProteomesiUP000002199: Chromosome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi108 – 1081S → A: Absence of autophosphorylation, retains kinase activity. 1 Publication
    Mutagenesisi196 – 1961D → A: Drastically reduced kinase activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 258258RIO-type serine/threonine-protein kinase Rio1PRO_0000344796Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei108 – 1081Phosphoserine; by autocatalysis1 Publication

    Keywords - PTMi

    Phosphoprotein

    PTM databases

    PhosSiteiP0507125.

    Interactioni

    Protein-protein interaction databases

    STRINGi224325.AF1804.

    Structurei

    Secondary structure

    1
    258
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 1310
    Helixi18 – 203
    Helixi23 – 308
    Helixi33 – 4412
    Beta strandi47 – 5711
    Beta strandi59 – 7012
    Beta strandi73 – 8210
    Helixi93 – 953
    Turni96 – 983
    Helixi104 – 1063
    Helixi110 – 12920
    Beta strandi137 – 1415
    Beta strandi144 – 1485
    Helixi160 – 1634
    Helixi164 – 1696
    Helixi172 – 18817
    Beta strandi192 – 1943
    Beta strandi201 – 21010
    Helixi213 – 2153
    Beta strandi216 – 2183
    Helixi224 – 24017
    Turni241 – 2433
    Helixi248 – 2569

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1ZP9X-ray2.00A/B/C/D1-258[»]
    1ZTFX-ray1.99A1-258[»]
    1ZTHX-ray1.89A/B/C/D1-258[»]
    3RE4X-ray2.00A/B1-258[»]
    4JINX-ray2.10A1-258[»]
    ProteinModelPortaliO28471.
    SMRiO28471. Positions 5-257.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO28471.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini49 – 258210Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1718.
    KOiK07178.
    OMAiGDPRFHL.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR018934. RIO-like_kinase.
    IPR000687. RIO_kinase.
    IPR018935. RIO_kinase_CS.
    [Graphical view]
    PfamiPF01163. RIO1. 1 hit.
    [Graphical view]
    SMARTiSM00090. RIO. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS01245. RIO1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O28471-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKDLKKIESY LDKLRIKEKD GEERKIYAEV LDGRTLKTLY KLSAKGYITA    50
    MGGVISTGKE ANVFYADGVF DGKPVAMAVK IYRIETSEFD KMDEYLYGDE 100
    RFDMRRISPK EKVFIWTEKE FRNLERAKEA GVSVPQPYTY MKNVLLMEFI 150
    GEDELPAPTL VELGRELKEL DVEGIFNDVV ENVKRLYQEA ELVHADLSEY 200
    NIMYIDKVYF IDMGQAVTLR HPMAESYLER DVRNIIRFFS KYGVKADFEE 250
    MLKEVKGE 258
    Length:258
    Mass (Da):30,095
    Last modified:January 1, 1998 - v1
    Checksum:i70CA739CA39A4863
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000782 Genomic DNA. Translation: AAB89445.1.
    PIRiC69475.
    RefSeqiNP_070631.1. NC_000917.1.

    Genome annotation databases

    EnsemblBacteriaiAAB89445; AAB89445; AF_1804.
    GeneIDi1485027.
    KEGGiafu:AF1804.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000782 Genomic DNA. Translation: AAB89445.1 .
    PIRi C69475.
    RefSeqi NP_070631.1. NC_000917.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1ZP9 X-ray 2.00 A/B/C/D 1-258 [» ]
    1ZTF X-ray 1.99 A 1-258 [» ]
    1ZTH X-ray 1.89 A/B/C/D 1-258 [» ]
    3RE4 X-ray 2.00 A/B 1-258 [» ]
    4JIN X-ray 2.10 A 1-258 [» ]
    ProteinModelPortali O28471.
    SMRi O28471. Positions 5-257.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224325.AF1804.

    PTM databases

    PhosSitei P0507125.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB89445 ; AAB89445 ; AF_1804 .
    GeneIDi 1485027.
    KEGGi afu:AF1804.

    Phylogenomic databases

    eggNOGi COG1718.
    KOi K07178.
    OMAi GDPRFHL.

    Enzyme and pathway databases

    BioCyci AFUL224325:GJBC-1839-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei O28471.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR018934. RIO-like_kinase.
    IPR000687. RIO_kinase.
    IPR018935. RIO_kinase_CS.
    [Graphical view ]
    Pfami PF01163. RIO1. 1 hit.
    [Graphical view ]
    SMARTi SM00090. RIO. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS01245. RIO1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus."
      Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G.
      , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C.
      Nature 390:364-370(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.
    2. "Structure and activity of the atypical serine kinase Rio1."
      Laronde-Leblanc N., Guszczynski T., Copeland T., Wlodawer A.
      FEBS J. 272:3698-3713(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 99-110, X-RAY CRYSTALLOGRAPHY (1.89 ANGSTROMS) IN PRESENCE AND ABSENCE OF ATP, PHOSPHORYLATION AT SER-108, MUTAGENESIS OF SER-108 AND ASP-196.
      Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

    Entry informationi

    Entry nameiRIO1_ARCFU
    AccessioniPrimary (citable) accession number: O28471
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 22, 2008
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3