Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O28471

- RIO1_ARCFU

UniProt

O28471 - RIO1_ARCFU

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

RIO-type serine/threonine-protein kinase Rio1

Gene

rio1

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Autophosphorylation of the rio1 protein is not necessary for maintenance of kinase activity. Prefers ATP over GTP. The yeast ortholog is involved in ribosome biogenesis.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Magnesium.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei80 – 801ATP
Binding sitei148 – 1481ATP; via carbonyl oxygen
Binding sitei150 – 1501ATP; via amide nitrogen
Active sitei196 – 1961Proton acceptorPROSITE-ProRule annotation
Metal bindingi201 – 2011Magnesium
Binding sitei201 – 2011ATP
Metal bindingi212 – 2121Magnesium
Binding sitei212 – 2121ATP

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi55 – 639ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. protein serine/threonine kinase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-1839-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
RIO-type serine/threonine-protein kinase Rio1 (EC:2.7.11.1)
Short name:
AfRio1
Gene namesi
Name:rio1
Ordered Locus Names:AF_1804
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
ProteomesiUP000002199: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi108 – 1081S → A: Absence of autophosphorylation, retains kinase activity. 1 Publication
Mutagenesisi196 – 1961D → A: Drastically reduced kinase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 258258RIO-type serine/threonine-protein kinase Rio1PRO_0000344796Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei108 – 1081Phosphoserine; by autocatalysis1 Publication

Keywords - PTMi

Phosphoprotein

PTM databases

PhosSiteiP0507125.

Interactioni

Protein-protein interaction databases

STRINGi224325.AF1804.

Structurei

Secondary structure

1
258
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 1310
Helixi18 – 203
Helixi23 – 308
Helixi33 – 4412
Beta strandi47 – 5711
Beta strandi59 – 7012
Beta strandi73 – 8210
Helixi93 – 953
Turni96 – 983
Helixi104 – 1063
Helixi110 – 12920
Beta strandi137 – 1415
Beta strandi144 – 1485
Helixi160 – 1634
Helixi164 – 1696
Helixi172 – 18817
Beta strandi192 – 1943
Beta strandi201 – 21010
Helixi213 – 2153
Beta strandi216 – 2183
Helixi224 – 24017
Turni241 – 2433
Helixi248 – 2569

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZP9X-ray2.00A/B/C/D1-258[»]
1ZTFX-ray1.99A1-258[»]
1ZTHX-ray1.89A/B/C/D1-258[»]
3RE4X-ray2.00A/B1-258[»]
4JINX-ray2.10A1-258[»]
ProteinModelPortaliO28471.
SMRiO28471. Positions 5-257.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO28471.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini49 – 258210Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG1718.
KOiK07178.
OMAiGDPRFHL.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR018934. RIO-like_kinase.
IPR000687. RIO_kinase.
IPR018935. RIO_kinase_CS.
[Graphical view]
PfamiPF01163. RIO1. 1 hit.
[Graphical view]
SMARTiSM00090. RIO. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS01245. RIO1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O28471 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKDLKKIESY LDKLRIKEKD GEERKIYAEV LDGRTLKTLY KLSAKGYITA
60 70 80 90 100
MGGVISTGKE ANVFYADGVF DGKPVAMAVK IYRIETSEFD KMDEYLYGDE
110 120 130 140 150
RFDMRRISPK EKVFIWTEKE FRNLERAKEA GVSVPQPYTY MKNVLLMEFI
160 170 180 190 200
GEDELPAPTL VELGRELKEL DVEGIFNDVV ENVKRLYQEA ELVHADLSEY
210 220 230 240 250
NIMYIDKVYF IDMGQAVTLR HPMAESYLER DVRNIIRFFS KYGVKADFEE

MLKEVKGE
Length:258
Mass (Da):30,095
Last modified:January 1, 1998 - v1
Checksum:i70CA739CA39A4863
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000782 Genomic DNA. Translation: AAB89445.1.
PIRiC69475.
RefSeqiNP_070631.1. NC_000917.1.
WP_010879299.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB89445; AAB89445; AF_1804.
GeneIDi1485027.
KEGGiafu:AF1804.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000782 Genomic DNA. Translation: AAB89445.1 .
PIRi C69475.
RefSeqi NP_070631.1. NC_000917.1.
WP_010879299.1. NC_000917.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1ZP9 X-ray 2.00 A/B/C/D 1-258 [» ]
1ZTF X-ray 1.99 A 1-258 [» ]
1ZTH X-ray 1.89 A/B/C/D 1-258 [» ]
3RE4 X-ray 2.00 A/B 1-258 [» ]
4JIN X-ray 2.10 A 1-258 [» ]
ProteinModelPortali O28471.
SMRi O28471. Positions 5-257.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224325.AF1804.

PTM databases

PhosSitei P0507125.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB89445 ; AAB89445 ; AF_1804 .
GeneIDi 1485027.
KEGGi afu:AF1804.

Phylogenomic databases

eggNOGi COG1718.
KOi K07178.
OMAi GDPRFHL.

Enzyme and pathway databases

BioCyci AFUL224325:GJBC-1839-MONOMER.

Miscellaneous databases

EvolutionaryTracei O28471.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR018934. RIO-like_kinase.
IPR000687. RIO_kinase.
IPR018935. RIO_kinase_CS.
[Graphical view ]
Pfami PF01163. RIO1. 1 hit.
[Graphical view ]
SMARTi SM00090. RIO. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS01245. RIO1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus."
    Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G.
    , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C.
    Nature 390:364-370(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.
  2. "Structure and activity of the atypical serine kinase Rio1."
    Laronde-Leblanc N., Guszczynski T., Copeland T., Wlodawer A.
    FEBS J. 272:3698-3713(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 99-110, X-RAY CRYSTALLOGRAPHY (1.89 ANGSTROMS) IN PRESENCE AND ABSENCE OF ATP, PHOSPHORYLATION AT SER-108, MUTAGENESIS OF SER-108 AND ASP-196.
    Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

Entry informationi

Entry nameiRIO1_ARCFU
AccessioniPrimary (citable) accession number: O28471
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: January 1, 1998
Last modified: October 29, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3