Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

RIO-type serine/threonine-protein kinase Rio1

Gene

rio1

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Autophosphorylation of the rio1 protein is not necessary for maintenance of kinase activity. Prefers ATP over GTP. The yeast ortholog is involved in ribosome biogenesis.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei80ATPPROSITE-ProRule annotationCombined sources1 Publication1
Binding sitei148ATP; via carbonyl oxygenCombined sources1 Publication1
Binding sitei150ATP; via amide nitrogenCombined sources1 Publication1
Active sitei196Proton acceptorPROSITE-ProRule annotation1
Binding sitei200ATP; via carbonyl oxygenBy similarity1
Metal bindingi201Magnesium1 Publication1
Binding sitei201ATPCombined sources1 Publication1
Metal bindingi212Magnesium1 Publication1
Binding sitei212ATPCombined sources1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi55 – 63ATPPROSITE-ProRule annotation9

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 414.

Names & Taxonomyi

Protein namesi
Recommended name:
RIO-type serine/threonine-protein kinase Rio1 (EC:2.7.11.1)
Short name:
AfRio1
Gene namesi
Name:rio1
Ordered Locus Names:AF_1804
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi108S → A: Absence of autophosphorylation, retains kinase activity. 1 Publication1
Mutagenesisi196D → A: Drastically reduced kinase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003447961 – 258RIO-type serine/threonine-protein kinase Rio1Add BLAST258

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei108Phosphoserine; by autocatalysis1 Publication1

Keywords - PTMi

Phosphoprotein

PTM databases

iPTMnetiO28471.

Interactioni

Protein-protein interaction databases

STRINGi224325.AF1804.

Structurei

Secondary structure

1258
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 13Combined sources10
Helixi18 – 20Combined sources3
Helixi23 – 30Combined sources8
Helixi33 – 44Combined sources12
Beta strandi47 – 57Combined sources11
Beta strandi59 – 70Combined sources12
Beta strandi73 – 82Combined sources10
Helixi93 – 95Combined sources3
Turni96 – 98Combined sources3
Helixi104 – 106Combined sources3
Helixi110 – 129Combined sources20
Beta strandi137 – 141Combined sources5
Beta strandi144 – 148Combined sources5
Helixi160 – 163Combined sources4
Helixi164 – 169Combined sources6
Helixi172 – 188Combined sources17
Beta strandi192 – 194Combined sources3
Beta strandi201 – 210Combined sources10
Helixi213 – 215Combined sources3
Beta strandi216 – 218Combined sources3
Helixi224 – 240Combined sources17
Turni241 – 243Combined sources3
Helixi248 – 256Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZP9X-ray2.00A/B/C/D1-258[»]
1ZTFX-ray1.99A1-258[»]
1ZTHX-ray1.89A/B/C/D1-258[»]
3RE4X-ray2.00A/B1-258[»]
4JINX-ray2.10A1-258[»]
ProteinModelPortaliO28471.
SMRiO28471.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO28471.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini49 – 258Protein kinasePROSITE-ProRule annotationAdd BLAST210

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiarCOG01180. Archaea.
COG1718. LUCA.
KOiK07178.
OMAiGDPRFHL.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR000687. RIO_kinase.
IPR018935. RIO_kinase_CS.
IPR017407. Ser/Thr_kinase_Rio1.
[Graphical view]
PANTHERiPTHR10593:SF23. PTHR10593:SF23. 1 hit.
SMARTiSM00090. RIO. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS01245. RIO1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O28471-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDLKKIESY LDKLRIKEKD GEERKIYAEV LDGRTLKTLY KLSAKGYITA
60 70 80 90 100
MGGVISTGKE ANVFYADGVF DGKPVAMAVK IYRIETSEFD KMDEYLYGDE
110 120 130 140 150
RFDMRRISPK EKVFIWTEKE FRNLERAKEA GVSVPQPYTY MKNVLLMEFI
160 170 180 190 200
GEDELPAPTL VELGRELKEL DVEGIFNDVV ENVKRLYQEA ELVHADLSEY
210 220 230 240 250
NIMYIDKVYF IDMGQAVTLR HPMAESYLER DVRNIIRFFS KYGVKADFEE

MLKEVKGE
Length:258
Mass (Da):30,095
Last modified:January 1, 1998 - v1
Checksum:i70CA739CA39A4863
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB89445.1.
PIRiC69475.
RefSeqiWP_010879299.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB89445; AAB89445; AF_1804.
GeneIDi24795547.
KEGGiafu:AF_1804.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB89445.1.
PIRiC69475.
RefSeqiWP_010879299.1. NC_000917.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZP9X-ray2.00A/B/C/D1-258[»]
1ZTFX-ray1.99A1-258[»]
1ZTHX-ray1.89A/B/C/D1-258[»]
3RE4X-ray2.00A/B1-258[»]
4JINX-ray2.10A1-258[»]
ProteinModelPortaliO28471.
SMRiO28471.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224325.AF1804.

PTM databases

iPTMnetiO28471.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB89445; AAB89445; AF_1804.
GeneIDi24795547.
KEGGiafu:AF_1804.

Phylogenomic databases

eggNOGiarCOG01180. Archaea.
COG1718. LUCA.
KOiK07178.
OMAiGDPRFHL.

Enzyme and pathway databases

BRENDAi2.7.11.1. 414.

Miscellaneous databases

EvolutionaryTraceiO28471.
PROiO28471.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR000687. RIO_kinase.
IPR018935. RIO_kinase_CS.
IPR017407. Ser/Thr_kinase_Rio1.
[Graphical view]
PANTHERiPTHR10593:SF23. PTHR10593:SF23. 1 hit.
SMARTiSM00090. RIO. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS01245. RIO1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRIO1_ARCFU
AccessioniPrimary (citable) accession number: O28471
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.