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O28471 (RIO1_ARCFU) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RIO-type serine/threonine-protein kinase Rio1

Short name=AfRio1
EC=2.7.11.1
Gene names
Name:rio1
Ordered Locus Names:AF_1804
OrganismArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) [Reference proteome] [HAMAP]
Taxonomic identifier224325 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus

Protein attributes

Sequence length258 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Autophosphorylation of the rio1 protein is not necessary for maintenance of kinase activity. Prefers ATP over GTP. The yeast ortholog is involved in ribosome biogenesis.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium.

Sequence similarities

Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 258258RIO-type serine/threonine-protein kinase Rio1
PRO_0000344796

Regions

Domain49 – 258210Protein kinase
Nucleotide binding55 – 639ATP By similarity

Sites

Active site1961Proton acceptor By similarity
Metal binding2011Magnesium
Metal binding2121Magnesium
Binding site801ATP
Binding site1481ATP; via carbonyl oxygen
Binding site1501ATP; via amide nitrogen
Binding site2011ATP
Binding site2121ATP

Amino acid modifications

Modified residue1081Phosphoserine; by autocatalysis Ref.2

Experimental info

Mutagenesis1081S → A: Absence of autophosphorylation, retains kinase activity. Ref.2
Mutagenesis1961D → A: Drastically reduced kinase activity. Ref.2

Secondary structure

........................................... 258
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O28471 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 70CA739CA39A4863

FASTA25830,095
        10         20         30         40         50         60 
MKDLKKIESY LDKLRIKEKD GEERKIYAEV LDGRTLKTLY KLSAKGYITA MGGVISTGKE 

        70         80         90        100        110        120 
ANVFYADGVF DGKPVAMAVK IYRIETSEFD KMDEYLYGDE RFDMRRISPK EKVFIWTEKE 

       130        140        150        160        170        180 
FRNLERAKEA GVSVPQPYTY MKNVLLMEFI GEDELPAPTL VELGRELKEL DVEGIFNDVV 

       190        200        210        220        230        240 
ENVKRLYQEA ELVHADLSEY NIMYIDKVYF IDMGQAVTLR HPMAESYLER DVRNIIRFFS 

       250 
KYGVKADFEE MLKEVKGE 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000782 Genomic DNA. Translation: AAB89445.1.
PIRC69475.
RefSeqNP_070631.1. NC_000917.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZP9X-ray2.00A/B/C/D1-258[»]
1ZTFX-ray1.99A1-258[»]
1ZTHX-ray1.89A/B/C/D1-258[»]
3RE4X-ray2.00A/B1-258[»]
4JINX-ray2.10A1-258[»]
ProteinModelPortalO28471.
SMRO28471. Positions 5-257.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224325.AF1804.

PTM databases

PhosSiteP0507125.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB89445; AAB89445; AF_1804.
GeneID1485027.
KEGGafu:AF1804.

Phylogenomic databases

eggNOGCOG1718.
KOK07178.
OMAGDPRFHL.
ProtClustDBCLSK2747046.

Enzyme and pathway databases

BioCycAFUL224325:GJBC-1839-MONOMER.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR018934. RIO-like_kinase.
IPR000687. RIO_kinase.
IPR018935. RIO_kinase_CS.
[Graphical view]
PfamPF01163. RIO1. 1 hit.
[Graphical view]
SMARTSM00090. RIO. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS01245. RIO1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceO28471.

Entry information

Entry nameRIO1_ARCFU
AccessionPrimary (citable) accession number: O28471
Entry history
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: January 1, 1998
Last modified: October 16, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references