Reviewed, UniProtKB/Swiss-Prot O28469 (TOP1_ARCFU)

Names and origin

Protein names

Recommended name:
    DNA topoisomerase 1
    EC=5.99.1.2
Alternative name(s):
    DNA topoisomerase I
    Omega-protein
    Relaxing enzyme
    Untwisting enzyme
    Swivelase

Gene names

Name:	topA
Ordered Locus Names:	AF_1806

Organism

Archaeoglobus fulgidus [Complete proteome] [HAMAP]

Taxonomic identifier

2234 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	663 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another By similarity.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Miscellaneous	When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand.
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family.

Ontologies

Keywords
Domain	Repeat Zinc-finger
Ligand	ATP-binding DNA-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA unwinding during replication Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 663	663	DNA topoisomerase 1		PRO_0000145177
Regions
	Zinc finger	583 – 610	28	C4-type 1
	Zinc finger	629 – 653	25	C4-type 2; atypical
Sites
	Active site	306	1	For DNA cleavage activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	O28469-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 0A07E4FD20871BAC	FASTA	663	76,018
	10 20 30 40 50 60 MVMSWLIITE KDNTARRIAS ILFKDVKTLK KGRVSYYHSP SNDAYVVGLK GHIVELDFPK 70 80 90 100 110 120 ELNNWTKTPL EKLLQAELVK KVKERTISSI LKEIAKKADR VTVATDYDRE GELIGVEALE 130 140 150 160 170 180 IVKSVNPTVK VDRVRYSAVT EKEIRSAFSK PVKVDFNLAN AALARQKIDL IWGATLTRLI 190 200 210 220 230 240 SVHSGRMGKD FLSVGRVQTP TLRLIVDREL EIQNFKPEKY YEIFAEFEGF IAKHPKRYGS 250 260 270 280 290 300 KEEAEKLFAK IGETAKVEEF TRRRMEENRP TPFNTTEFLR EASKFMSPHK AMNIAETLYM 310 320 330 340 350 360 NGYISYPRTD NTVYPPTINL IEIVSALSSV FPREAQIVLS QDKITPSRGR RETKDHPPIY 370 380 390 400 410 420 PTGVAKRGEL SKDEWTIYEL VVRRFLATLA PKAVWDVRRV VLDSNGVKFV ANGRQLVEAG 430 440 450 460 470 480 WRDIYIYSKA EETELPLLKK GDVLRILKKR LEEKETKPPG RYSASSLIKM MEKLNLGTKS 490 500 510 520 530 540 TRHEIIQKLV SRRYIHGNPF RPSETAFSVI NVLKETAETI TLPDMTAKLE NEMDLIAEGK 550 560 570 580 590 600 KREPEVVDES REMLLQILRA IDYRKLSKDL REGVKKDKIV GKCPECGGEL VVRQSKAGKR 610 620 630 640 650 660 FIGCSNYPDC TFTLPLPQNG TLYITAKQCK EHEIKEVKIR TKKGYWNLGC PYCNYLNWKK ENS

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000782 Genomic DNA. Translation: AAB89443.1.
PIR	E69475.
RefSeq	NP_070633.1.
3D structure databases
HSSP	HSSP built from PDB template 1D6M based on UniProtKB P14294.
ModBase	Search...
Genome annotation databases
GeneID	1485029.
GenomeReviews	Gene locus AF_1806 in contig AE000782_GR.
KEGG	afu:AF1806.
NMPDR	fig|224325.1.peg.1794.
TIGR	AF_1806.
Phylogenomic databases
HOGENOM	O28469.
OMA	LIICEKP.
Enzyme and pathway databases
BioCyc	AFUL224325:AF_1806-MON.
BRENDA	5.99.1.2. 7576.
Family and domain databases
InterPro	IPR003601. Topo_IA_2. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013826. Topo_IA_cen_sub3. IPR000380. Topo_IA_core. IPR003602. Topo_IA_DNA_bd. IPR013498. Topo_IA_Znf. IPR005739. TopoI_arch. IPR006154. Toprim_dom_subgr. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 1 hit. G3DSA:1.10.290.10. Topo_IA_cen_sub3. 1 hit.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
TIGRFAMs	TIGR01057. topA_arch. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TOP1_ARCFU

Accession

Primary (citable) accession number: O28469

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	January 1, 1998
Last modified:	November 3, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)