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Protein

Probable L-aspartate dehydrogenase

Gene

nadX

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate.UniRule annotation

Catalytic activityi

L-aspartate + H2O + NAD(P)+ = oxaloacetate + NH3 + NAD(P)H.UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes iminoaspartate from L-aspartate (dehydrogenase route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Probable L-aspartate dehydrogenase (nadX)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes iminoaspartate from L-aspartate (dehydrogenase route), the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei111NAD; via amide nitrogenUniRule annotation1
Binding sitei162NADUniRule annotation1
Active sitei189UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Keywords - Ligandi

NAD, NADP

Enzyme and pathway databases

BRENDAi1.4.1.21. 414.
SABIO-RKO28440.
UniPathwayiUPA00253; UER00456.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable L-aspartate dehydrogenaseUniRule annotation (EC:1.4.1.21UniRule annotation)
Gene namesi
Name:nadXUniRule annotation
Ordered Locus Names:AF_1838
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001448941 – 236Probable L-aspartate dehydrogenaseAdd BLAST236

Interactioni

Protein-protein interaction databases

STRINGi224325.AF1838.

Structurei

Secondary structure

1236
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 6Combined sources5
Helixi10 – 21Combined sources12
Beta strandi25 – 30Combined sources6
Beta strandi39 – 42Combined sources4
Helixi43 – 46Combined sources4
Beta strandi52 – 56Combined sources5
Helixi60 – 72Combined sources13
Beta strandi76 – 80Combined sources5
Helixi82 – 86Combined sources5
Helixi88 – 101Combined sources14
Beta strandi105 – 107Combined sources3
Helixi115 – 120Combined sources6
Helixi122 – 124Combined sources3
Beta strandi125 – 135Combined sources11
Helixi136 – 138Combined sources3
Beta strandi143 – 149Combined sources7
Helixi150 – 156Combined sources7
Helixi162 – 171Combined sources10
Beta strandi176 – 183Combined sources8
Beta strandi185 – 195Combined sources11
Beta strandi198 – 206Combined sources9
Beta strandi212 – 216Combined sources5
Helixi217 – 231Combined sources15
Beta strandi232 – 235Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DC1X-ray1.90A/B1-236[»]
ProteinModelPortaliO28440.
SMRiO28440.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO28440.

Family & Domainsi

Sequence similaritiesi

Belongs to the L-aspartate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00254. Archaea.
COG1712. LUCA.
KOiK06989.
OMAiMIMSVGA.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01265. NadX. 1 hit.
InterProiIPR005106. Asp/hSer_DH_NAD-bd.
IPR002811. Asp_DH.
IPR022487. Asp_DH_arc.
IPR020626. Asp_DH_prok.
IPR011182. L-Asp_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01958. DUF108. 1 hit.
PF03447. NAD_binding_3. 1 hit.
[Graphical view]
PIRSFiPIRSF005227. Asp_dh_NAD_syn. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR03855. NAD_NadX. 1 hit.

Sequencei

Sequence statusi: Complete.

O28440-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVGLIGYGA IGKFLAEWLE RNGFEIAAIL DVRGEHEKMV RGIDEFLQRE
60 70 80 90 100
MDVAVEAASQ QAVKDYAEKI LKAGIDLIVL STGAFADRDF LSRVREVCRK
110 120 130 140 150
TGRRVYIASG AIGGLDAIFS ASELIEEIVL TTRKNWRQFG RKGVIFEGSA
160 170 180 190 200
SEAAQKFPKN LNVAATLSIA SGKDVKVRLV ADEVEENIHE ILVRGEFGEM
210 220 230
EIRVRNRPMR ENPKTSYLAA LSVTRILRNL KEGLVV
Length:236
Mass (Da):26,208
Last modified:January 1, 1998 - v1
Checksum:i29218ACC7F919F52
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB89415.1.
PIRiE69479.
RefSeqiWP_010879332.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB89415; AAB89415; AF_1838.
GeneIDi24795582.
KEGGiafu:AF_1838.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB89415.1.
PIRiE69479.
RefSeqiWP_010879332.1. NC_000917.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DC1X-ray1.90A/B1-236[»]
ProteinModelPortaliO28440.
SMRiO28440.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224325.AF1838.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB89415; AAB89415; AF_1838.
GeneIDi24795582.
KEGGiafu:AF_1838.

Phylogenomic databases

eggNOGiarCOG00254. Archaea.
COG1712. LUCA.
KOiK06989.
OMAiMIMSVGA.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00456.
BRENDAi1.4.1.21. 414.
SABIO-RKO28440.

Miscellaneous databases

EvolutionaryTraceiO28440.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01265. NadX. 1 hit.
InterProiIPR005106. Asp/hSer_DH_NAD-bd.
IPR002811. Asp_DH.
IPR022487. Asp_DH_arc.
IPR020626. Asp_DH_prok.
IPR011182. L-Asp_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01958. DUF108. 1 hit.
PF03447. NAD_binding_3. 1 hit.
[Graphical view]
PIRSFiPIRSF005227. Asp_dh_NAD_syn. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR03855. NAD_NadX. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiASPD_ARCFU
AccessioniPrimary (citable) accession number: O28440
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia.UniRule annotation

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.