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Protein

Methionine aminopeptidase

Gene

map

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).UniRule annotation

Catalytic activityi

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

Cofactori

Co2+UniRule annotation, Zn2+UniRule annotation, Mn2+UniRule annotation, Fe2+UniRule annotationNote: Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei65SubstrateUniRule annotation1
Metal bindingi85Divalent metal cation 1UniRule annotation1
Metal bindingi96Divalent metal cation 1UniRule annotation1
Metal bindingi96Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi155Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation1
Binding sitei163SubstrateUniRule annotation1
Metal bindingi188Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi276Divalent metal cation 1UniRule annotation1
Metal bindingi276Divalent metal cation 2; catalyticUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Protease
LigandMetal-binding

Protein family/group databases

MEROPSiM24.035

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminopeptidaseUniRule annotation (EC:3.4.11.18UniRule annotation)
Short name:
MAPUniRule annotation
Short name:
MetAPUniRule annotation
Alternative name(s):
Peptidase MUniRule annotation
Gene namesi
Name:mapUniRule annotation
Ordered Locus Names:AF_1840
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001489731 – 291Methionine aminopeptidaseAdd BLAST291

Proteomic databases

PRIDEiO28438

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi224325.AF1840

Structurei

3D structure databases

ProteinModelPortaliO28438
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M24A family. Methionine aminopeptidase archaeal type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01001 Archaea
COG0024 LUCA
KOiK01265
OMAiHTVLLMP
OrthoDBiPOG093Z06FJ

Family and domain databases

CDDicd01088 MetAP2, 1 hit
Gene3Di1.10.10.10, 1 hit
HAMAPiMF_01975 MetAP_2_arc, 1 hit
InterProiView protein in InterPro
IPR036005 Creatinase/aminopeptidase-like
IPR028595 MetAP_archaeal
IPR000994 Pept_M24
IPR001714 Pept_M24_MAP
IPR002468 Pept_M24A_MAP2
IPR018349 Pept_M24A_MAP2_BS
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00557 Peptidase_M24, 1 hit
PRINTSiPR00599 MAPEPTIDASE
SUPFAMiSSF46785 SSF46785, 1 hit
SSF55920 SSF55920, 2 hits
TIGRFAMsiTIGR00501 met_pdase_II, 1 hit
PROSITEiView protein in PROSITE
PS01202 MAP_2, 1 hit

Sequencei

Sequence statusi: Complete.

O28438-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDEVREKLI EAGKILKQAV NEAAEKIAPG VKILEVAEFV ENRIIELGAK
60 70 80 90 100
PAFPANISIN SDAAHFTPKK NDERTFKEGD VVKLDVGAHI DGYIADMAVT
110 120 130 140 150
VDLGDNTELV KAAKEALEAA MEVVRAGVSV SEIGKAIEDA ITNYGFKPIV
160 170 180 190 200
NLTGHGLLPY LNHAPPSIYN YATEKGVTLE EGMVVAIEPF ATNGVGKVGE
210 220 230 240 250
RGECEIYSLL NPRPVRMKMA REILKEVEEN YKTLPFAKRW LKKAPDIIIS
260 270 280 290
KLAREGVLRA YPVLTEVSGG LVSQWEHTLI VEDGGATITT K
Length:291
Mass (Da):31,691
Last modified:January 1, 1998 - v1
Checksum:i5E621BDBDAAB7040
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA Translation: AAB89413.1
PIRiG69479
RefSeqiWP_010879334.1, NC_000917.1

Genome annotation databases

EnsemblBacteriaiAAB89413; AAB89413; AF_1840
GeneIDi24795584
KEGGiafu:AF_1840

Similar proteinsi

Entry informationi

Entry nameiMAP2_ARCFU
AccessioniPrimary (citable) accession number: O28438
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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