O28438 (AMPM_ARCFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine aminopeptidase
Short name=MAP
EC=3.4.11.18

Alternative name(s):
Peptidase M

Gene names

Name:	map
Ordered Locus Names:	AF_1840

Organism

Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) [Reference proteome] [HAMAP]

Taxonomic identifier

224325 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus › Archaeoglobus fulgidus

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Removes the N-terminal methionine from nascent proteins By similarity.
Catalytic activity	Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Cofactor	Binds 2 cobalt ions per subunit. The true nature of the physiological cofactor is under debate. The enzyme is also active with zinc, manganese or divalent iron ions By similarity.
Sequence similarities	Belongs to the peptidase M24A family.

Ontologies

Keywords
Ligand	Cobalt Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 291	291	Methionine aminopeptidase		PRO_0000148973
Sites
	Metal binding	85	1	Cobalt 1 By similarity
	Metal binding	96	1	Cobalt 1 By similarity
	Metal binding	96	1	Cobalt 2 By similarity
	Metal binding	155	1	Cobalt 2 By similarity
	Metal binding	188	1	Cobalt 2 By similarity
	Metal binding	276	1	Cobalt 1 By similarity
	Metal binding	276	1	Cobalt 2 By similarity
	Binding site	65	1	Substrate By similarity
	Binding site	163	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O28438 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 5E621BDBDAAB7040	FASTA	291	31,691
	10 20 30 40 50 60 MDDEVREKLI EAGKILKQAV NEAAEKIAPG VKILEVAEFV ENRIIELGAK PAFPANISIN 70 80 90 100 110 120 SDAAHFTPKK NDERTFKEGD VVKLDVGAHI DGYIADMAVT VDLGDNTELV KAAKEALEAA 130 140 150 160 170 180 MEVVRAGVSV SEIGKAIEDA ITNYGFKPIV NLTGHGLLPY LNHAPPSIYN YATEKGVTLE 190 200 210 220 230 240 EGMVVAIEPF ATNGVGKVGE RGECEIYSLL NPRPVRMKMA REILKEVEEN YKTLPFAKRW 250 260 270 280 290 LKKAPDIIIS KLAREGVLRA YPVLTEVSGG LVSQWEHTLI VEDGGATITT K

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000782 Genomic DNA. Translation: AAB89413.1.
PIR	G69479.
RefSeq	NP_070666.1. NC_000917.1.
3D structure databases
ProteinModelPortal	O28438.
ModBase	Search...
Protein-protein interaction databases
STRING	224325.AF1840.
Protein family/group databases
MEROPS	M24.035.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB89413; AAB89413; AF_1840.
GeneID	1485061.
KEGG	afu:AF1840.
Phylogenomic databases
eggNOG	COG0024.
KO	K01265.
OMA	GHKIERY.
ProtClustDB	PRK08671.
Enzyme and pathway databases
BioCyc	AFUL224325:GJBC-1875-MONOMER.
Family and domain databases
Gene3D	1.10.10.10. 1 hit. 3.90.230.10. 2 hits.
InterPro	IPR001714. Pept_M24_MAP. IPR000994. Pept_M24_structural-domain. IPR002468. Pept_M24A_MAP2. IPR018349. Pept_M24A_MAP2_BS. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF00557. Peptidase_M24. 1 hit. [Graphical view]
PRINTS	PR00599. MAPEPTIDASE.
SUPFAM	SSF55920. Peptidase_M24_cat_core. 1 hit.
TIGRFAMs	TIGR00501. met_pdase_II. 1 hit.
PROSITE	PS01202. MAP_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPM_ARCFU

Accession

Primary (citable) accession number: O28438

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program