Carbon monoxide dehydrogenase - Archaeoglobus fulgidus

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Reviewed, UniProtKB/Swiss-Prot O28429 (COOS_ARCFU)

Last modified June 16, 2009. Version 52. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
    Carbon monoxide dehydrogenase
      Short name=CODH
    EC=1.2.99.2

Gene names

Name:	cooS
Ordered Locus Names:	AF_1849

Organism

Archaeoglobus fulgidus [Complete proteome] [HAMAP]

Taxonomic identifier

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	622 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	CO + H₂O + A = CO₂ + AH₂.
Cofactor	Binds 3 4Fe-4S clusters per homodimer By similarity. Binds 2 nickel-iron-sulfur clusters per homodimer By similarity.
Subunit structure	Homodimer By similarity.
Domain	Cluster B is an all-cysteinyl-liganded 4Fe4S cluster; cluster C is a mixed Ni-Fe-S cluster which appears to be the active site of CO oxidation. Cluster D is also an all-cysteinyl-liganded 4Fe4S cluster that bridges the two subunits of the CODH dimer By similarity.
Sequence similarities	Belongs to the Ni-containing carbon monoxide dehydrogenase family.
Caution	This protein lacks the conserved Cys in positions 48 and 292; they are replaced by a Ser and a Glu, respectively. It is therefore possible that the C- and D-clusters are either altered or missing in this protein, which may not form homodimers.

Ontologies

Keywords
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Nickel
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	generation of precursor metabolites and energy Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW carbon-monoxide dehydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

622

Carbon monoxide dehydrogenase

PRO_0000157140

Sites

Metal binding

1

Iron-sulfur 1 (4Fe-4S); shared with dimeric partner By similarity

Metal binding

1

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

1

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

1

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

1

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

1

Nickel-iron-sulfur By similarity

Metal binding

1

Nickel-iron-sulfur By similarity

Metal binding

1

Nickel-iron-sulfur By similarity

Metal binding

1

Nickel-iron-sulfur By similarity

Metal binding

1

Nickel-iron-sulfur By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

O28429-1 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 018304F4C49177B4
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622

67,496

        10         20         30         40         50         60 
MKIEGKVSEH ESINMMYERV SKEGVTNIVD RFNAQEKGRC PFCEKGLSCQ LCSMGPCRIS 

        70         80         90        100        110        120 
KDKPTGACGI DAAGMVVRNF THKNMLGTEA YTYHAIEAAK TLKATAEGKT IYEIKDVEKL 

       130        140        150        160        170        180 
KWFAKLLGIE GEDVNELAAK VADFVISDLS SLEKSRLVEI FAPEKRKELW EKLGIFPSGV 

       190        200        210        220        230        240 
FQELLTMGSS AMTNVDSNYV SLAKKSMSMS IATCMAAQIA LETIQDILFG TPMPHESHSD 

       250        260        270        280        290        300 
LGILDPEYVN IAVNGHEPFV GIALIKLAER EEIQEKARKA GAKGLRIIGF IETGQEILQR 

       310        320        330        340        350        360 
VDSPVFAGIV GNWIVQEYAL ATGCVDVFAA DMNCTLPSLP EYQRYGVKIV PVSRLVRLKG 

       370        380        390        400        410        420 
IDEGLDYEPE KAEEIAMKLI DMAIENFKQR DKSKAVKVEQ KKKIVVGFSP EAILKALNGD 

       430        440        450        460        470        480 
LNVLLDAIKK GDIKGVVALV SCTTLKNGPH DSSTVTIAKE LIKRDILVLS MGCGNAALQV 

       490        500        510        520        530        540 
AGLTSMEAVE LAGEKLKAVC KALNIPPVLS FGTCTDTGRA AYLVRLIADA LGVDVPQLPV 

       550        560        570        580        590        600 
AVTAPEYMEQ KATIDAVFAV AYGLTTHVSP VPPITGSEDA VKLFTEDVEK LTGGKVVVEE 

       610        620 
DPLKAAELLE KVIEEKRKAL GI

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus."
Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G.

expand/collapse author list

Venter J.C.
Nature 390:364-370(1997) [PubMed: 9389475] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

Cross-references

Sequence databases
	AE000782 Genomic DNA. Translation: AAB89403.1.
PIR	H69480.
RefSeq	NP_070675.1.
3D structure databases
HSSP	HSSP built from PDB template 1JJY based on UniProtKB Q9F8A8.
ModBase	Search...
Genome annotation databases
GeneID	1485070.
GenomeReviews	Gene locus AF_1849 in contig AE000782_GR.
KEGG	afu:AF1849.
NMPDR	fig\|224325.1.peg.1836.
TIGR	AF_1849.
Phylogenomic databases
HOGENOM	O28429.
OMA	O28429. YMEQKAT.
Enzyme and pathway databases
BioCyc	AFUL224325:AF_1849-MON.
BRENDA	1.2.99.2. 7576.
Family and domain databases
InterPro	IPR016101. CO_DH_a-bundle. IPR010047. CO_DH_cat. IPR004137. Prismane. IPR016099. Prismane-like_a/b-sand. [Graphical view]
Gene3D	G3DSA:1.20.1270.30. CO_DH_a-bundle. 1 hit. G3DSA:3.40.50.2030. Prismane-like_a/b-sand. 1 hit.
Pfam	PF03063. Prismane. 1 hit. [Graphical view]
PIRSF	PIRSF005023. CODH. 1 hit.
TIGRFAMs	TIGR01702. CO_DH_cata. 1 hit.
ProtoNet	Search...

Entry information

Entry name

COOS_ARCFU

Accession

Primary (citable) accession number: O28429

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2003
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents