Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Elongation factor 2

Gene

fusA

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi27 – 348GTPBy similarity
Nucleotide bindingi93 – 975GTPBy similarity
Nucleotide bindingi147 – 1504GTPBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-1931-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 2
Short name:
EF-2
Gene namesi
Name:fusA
Synonyms:fus
Ordered Locus Names:AF_1894
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 728728Elongation factor 2PRO_0000091026Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei594 – 5941DiphthamideBy similarity

Proteomic databases

PRIDEiO28385.

Interactioni

Protein-protein interaction databases

STRINGi224325.AF1894.

Structurei

3D structure databases

ProteinModelPortaliO28385.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini18 – 258241tr-type GAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG01559. Archaea.
COG0480. LUCA.
KOiK03234.
OMAiFTGHVTR.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00054_A. EF_G_EF_2_A.
InterProiIPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004543. Transl_elong_EFG/EF2_arc.
IPR005517. Transl_elong_EFG/EF2_IV.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00490. aEF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O28385-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARAKKVDKI KELMWKPKFI RNIGIVAHID HGKTTLSDNL LAGAGMISEE
60 70 80 90 100
LAGQQLYLDF DEQEQERGIT INAANVSMVH EYEGQDYLIN LIDTPGHVDF
110 120 130 140 150
GGDVTRAMRA VDGVIVVVDA VEGVMPQTET VLRQALKENV KPVLFVNKVD
160 170 180 190 200
RLIKELELTP QQMQERLIKV ITEVNKLIKA MRPDKYSEWK IDVANGSAAF
210 220 230 240 250
GSALYNWAVS VPSQKKTGIG FKEVYEHIKE GKVKELAKKS PLYQVVLDMV
260 270 280 290 300
IRHLPSPIEA QKERIAVIWK GDINSEVGKA MVNCDPKGPV ALMITKIVVE
310 320 330 340 350
PQAGEIAVGR LYSGTIRPGM ELYIVDRKAK NRIQTVGLYM GPRRVEVDEI
360 370 380 390 400
PAGNIVAVIG LKDAVAGSTC TTVENLTPFE SIKHYSEPVV TMAIEAKNPR
410 420 430 440 450
DLPKLIEVLR KLAKEDPTLH ITLNEETGEH LISGMGELHL EVKVEKIRRD
460 470 480 490 500
YKLDVITSPP IVVFRETVTG TSPVVEGKSP NKHNRFYIVV EPLPEKVIQM
510 520 530 540 550
FKEGVVDMKM DKKERRRLLQ EAGLTSEEAA GAEEYYEGNL FCDVTKGIQY
560 570 580 590 600
LNETMELILE GFREAMRAGP IAREPCMGIK VKLVDCKLHE DAVHRGPAQV
610 620 630 640 650
IPAVRSAIFA AILQAKPALL EPYQKIFITV PQDMMGAVTR EIQGRRGQIL
660 670 680 690 700
EMKTEGDMVT IIAKAPVKEM FGFAGAIRGA TSGKAIWSTE HAGFELVPQN
710 720
LFQEFVMEVR KRKGLKLEMP KPEDFVGL
Length:728
Mass (Da):81,136
Last modified:January 1, 1998 - v1
Checksum:i62BA963D6571AE9C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB89360.1.
PIRiE69486.
RefSeqiWP_010879387.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB89360; AAB89360; AF_1894.
GeneIDi24795638.
KEGGiafu:AF_1894.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB89360.1.
PIRiE69486.
RefSeqiWP_010879387.1. NC_000917.1.

3D structure databases

ProteinModelPortaliO28385.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224325.AF1894.

Proteomic databases

PRIDEiO28385.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB89360; AAB89360; AF_1894.
GeneIDi24795638.
KEGGiafu:AF_1894.

Phylogenomic databases

eggNOGiarCOG01559. Archaea.
COG0480. LUCA.
KOiK03234.
OMAiFTGHVTR.

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-1931-MONOMER.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00054_A. EF_G_EF_2_A.
InterProiIPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004543. Transl_elong_EFG/EF2_arc.
IPR005517. Transl_elong_EFG/EF2_IV.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00490. aEF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus."
    Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G.
    , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C.
    Nature 390:364-370(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

Entry informationi

Entry nameiEF2_ARCFU
AccessioniPrimary (citable) accession number: O28385
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: July 6, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.