O28339 (PURL_ARCFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	AF_1940

Organism

Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)

Taxonomic identifier

224325 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	765 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 765	765	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_0000100509
Regions
	Nucleotide binding	129 – 140	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	O28339 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: C2C08CADF5C21AC1	FASTA	765	84,764
	10 20 30 40 50 60 MYRKVDVPFE LYEVEILDAS EEELAKISEE MGLALSVDEM KRIQDYFRQK GRNPYDIELQ 70 80 90 100 110 120 SLAQAWSEHC CYKSSKYYLR QYLLEASKAD YVISAIEEDA GVVEFDDEYA YVTAFESHNH 130 140 150 160 170 180 PSAIEPYGGA ATGIGGILRD VLCMGAQPIA LIDPLFFGNL DTPYEKLPRG TKHPLYLLKG 190 200 210 220 230 240 VVAGIRDYGN RVGIPTVAGM VFFDNSYLTN CLVNVGCVGI VRKDRIIHSR AGGAGDLFVL 250 260 270 280 290 300 AGGKTGRDGI HGVTFASAEL DEKSEEEARG AVQLGNPIMK EPLIHACLEV VEKGLLTGMK 310 320 330 340 350 360 DLGGGGLSCV IGEMALAAGF GAEVYLDKVP LKEEGMAPWE IWISESQERM MLTVRPEHID 370 380 390 400 410 420 EVLYIFQKWD VPATVVGKVI PEKITRIYYK GYKIYEMDTE FVTSGPEYCR PYVARKPEKE 430 440 450 460 470 480 LHEEVEPPAD YVKTFMKMLS HPNAAFKEWI VRQYDHEVRA STVLKPLQGK MNFETHGDAA 490 500 510 520 530 540 VIKPTRSFRG LAITADVNPW MCKVDPYWGA ASSFDEMVRN LVAVNAVPHS FNDCLNFGNP 550 560 570 580 590 600 EKPERMGEFV EAVKALGWMA KGVGVPCVSG NVSFYNETPY GAVAPTPSLL GVGIVEDVRK 610 620 630 640 650 660 AITSEFKGRG AVILVGETHN EFGGSLYSAV MGQRCHKVPR TSPERLKTYS DAMLESFRKF 670 680 690 700 710 720 EVKACHDVSM GGLAVCIAEM SFGRGLGFEA ARELSFVELF SESNTRWVVE VPESVAEGYA 730 740 750 760 EFFRAKELKA EVIGYSGGER LDFGAFSVEL GEADKSWREG LTKFL

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000782 Genomic DNA. Translation: AAB89311.1.
PIR	C69492.
RefSeq	NP_070765.1. NC_000917.1.
3D structure databases
ProteinModelPortal	O28339.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1485163.
GenomeReviews	Gene locus AF_1940 in contig AE000782_GR.
KEGG	afu:AF1940.
NMPDR	fig|224325.1.peg.1926.
TIGR	AF_1940.
Phylogenomic databases
HOGENOM	HBG311214.
OMA	YGNSFGV.
PhylomeDB	O28339.
ProtClustDB	PRK01213.
Enzyme and pathway databases
BioCyc	AFUL224325:AF_1940-MONOMER.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_ARCFU

Accession

Primary (citable) accession number: O28339

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2001
Last sequence update:	January 1, 1998
Last modified:	January 25, 2012
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program