O28324 (SYFA_ARCFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase alpha chain
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase alpha chain
Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	AF_1955

Organism

Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)

Taxonomic identifier

224325 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	474 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00282
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00282
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm HAMAP MF_00282.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 474	474	Phenylalanine--tRNA ligase alpha chain HAMAP MF_00282		PRO_0000126807

Sequences

	Sequence		Length	Mass (Da)
	O28324 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 9937737D67BF8BE5	FASTA	474	54,582
	10 20 30 40 50 60 MLSKIELMLL RSLETGKTYT PEEAAELSGL SKDAVLKAAY MLQERGYVRV IENVRTHYSL 70 80 90 100 110 120 TEEGERYLKE GLPEEKLYSL VKSGVNSMDE LRKRMGREFQ IALGWLRRKG AVEVEGDKVV 130 140 150 160 170 180 PKREPSFDER AALQAIKEGR GVDEGTLKTL MKRKLVTKSE TKQVYIEVMK KPEIELEDII 190 200 210 220 230 240 TDLTPEMLLE GTWRGKQFLK YDITIPAKEI YGGKIHPYER IIEECRKVFL EMGFTEIKGH 250 260 270 280 290 300 YVQPAFWNFD ALFQPQDHPA RDMQDTFYLD RYIDLEGEIV ERVKATHENG WITGSTGWGG 310 320 330 340 350 360 EWSLEKARQL VLRTHTTAIT IHYLAENPEP PQKAFCIDRV YRRETIDATH LPEFDQLEGV 370 380 390 400 410 420 VLDRDVGFKH LLGLLKEFFT KMGFEDVRFR PGYFPYTEPS VEPEVYVEGL GWVELGGAGV 430 440 450 460 470 FRKEVTEPLG IRGKVLAWGL GIGRLAMLKI GLKDLRRLYL PDLGWLRSMP VARK

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000782 Genomic DNA. Translation: AAB89300.1.
PIR	B69494.
RefSeq	NP_070779.1. NC_000917.1.
3D structure databases
ProteinModelPortal	O28324.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1485176.
GenomeReviews	Gene locus AF_1955 in contig AE000782_GR.
KEGG	afu:AF1955.
NMPDR	fig|224325.1.peg.1940.
TIGR	AF_1955.
Phylogenomic databases
HOGENOM	HBG497668.
OMA	FTIGRVF.
PhylomeDB	O28324.
ProtClustDB	PRK04172.
Enzyme and pathway databases
BioCyc	AFUL224325:AF_1955-MONOMER.
Family and domain databases
HAMAP	MF_00282. Phe_tRNA_synth_alpha2. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004529. Phe-tRNA-synth_IIc_asu. IPR022917. Phe_tRNA_synth_alpha2_bac/arc. IPR002319. Phenylalanyl-tRNA_Synthase. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K01889.
Pfam	PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
TIGRFAMs	TIGR00468. PheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_ARCFU

Accession

Primary (citable) accession number: O28324

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 8, 2000
Last sequence update:	January 1, 1998
Last modified:	January 25, 2012
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program