Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Holliday junction resolvase Hjc

Gene

hjc

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination. Cleaves 4-way DNA junctions introducing paired nicks in opposing strands, leaving a 5'-terminal phosphate and a 3'-terminal hydroxyl group that are ligated to produce recombinant products.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi9MagnesiumUniRule annotation1
Active sitei29UniRule annotation1
Metal bindingi38MagnesiumUniRule annotation1
Metal bindingi51MagnesiumUniRule annotation1
Sitei53Transition state stabilizerSequence analysis1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Holliday junction resolvase HjcUniRule annotation (EC:3.1.22.4UniRule annotation)
Short name:
HjcUniRule annotation
Gene namesi
Name:hjcUniRule annotation
Ordered Locus Names:AF_1965
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004291531 – 136Holliday junction resolvase HjcAdd BLAST136

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi224325.AF1965.

Structurei

Secondary structure

1136
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 18Combined sources14
Beta strandi22 – 26Combined sources5
Beta strandi29 – 31Combined sources3
Beta strandi32 – 34Combined sources3
Beta strandi38 – 42Combined sources5
Beta strandi47 – 54Combined sources8
Beta strandi56 – 58Combined sources3
Beta strandi60 – 63Combined sources4
Helixi64 – 77Combined sources14
Beta strandi80 – 86Combined sources7
Beta strandi93 – 96Combined sources4
Helixi97 – 99Combined sources3
Beta strandi100 – 102Combined sources3
Beta strandi104 – 109Combined sources6
Turni111 – 113Combined sources3
Helixi114 – 116Combined sources3
Helixi120 – 125Combined sources6
Helixi126 – 128Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WCWX-ray1.58A/B/C/D2-136[»]
2WCZX-ray1.65A/B2-136[»]
2WIWX-ray1.80A/B2-136[»]
2WIZX-ray3.30A/B2-136[»]
2WJ0X-ray3.10A/B2-136[»]
ProteinModelPortaliO28314.
SMRiO28314.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO28314.

Family & Domainsi

Sequence similaritiesi

Belongs to the Holliday junction resolvase Hjc family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00919. Archaea.
COG1591. LUCA.
KOiK03552.
OMAiPVLAVKF.

Family and domain databases

CDDicd00523. archeal_HJR. 1 hit.
Gene3Di3.40.1350.10. 1 hit.
HAMAPiMF_01490. HJ_Resolv_Hjc. 1 hit.
InterProiIPR002732. Hjc.
IPR014428. Hjc_arc.
IPR011335. Restrct_endonuc-II-like.
IPR011856. tRNA_endonuc-like_dom.
[Graphical view]
PfamiPF01870. Hjc. 1 hit.
[Graphical view]
PIRSFiPIRSF004985. Hlld_jn_rslvs_ar. 1 hit.
ProDomiPD015874. PD015874. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

O28314-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSKGTRFER DLLVELWKAG FAAIRVAGSG VSPFPCPDIV AGNGRTYLAI
60 70 80 90 100
EVKMRKELPL YLSADEVEQL VTFARGFGAE AYVALKLPRK KWRFFPVQML
110 120 130
ERTEKNFKID ESVYPLGLEI AEVAGKFFQE RFGEKV
Length:136
Mass (Da):15,437
Last modified:January 1, 1998 - v1
Checksum:iC59D1FF6ABDE1AC8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB89293.1.
PIRiD69495.
RefSeqiWP_010879457.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB89293; AAB89293; AF_1965.
GeneIDi24795708.
KEGGiafu:AF_1965.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB89293.1.
PIRiD69495.
RefSeqiWP_010879457.1. NC_000917.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WCWX-ray1.58A/B/C/D2-136[»]
2WCZX-ray1.65A/B2-136[»]
2WIWX-ray1.80A/B2-136[»]
2WIZX-ray3.30A/B2-136[»]
2WJ0X-ray3.10A/B2-136[»]
ProteinModelPortaliO28314.
SMRiO28314.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224325.AF1965.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB89293; AAB89293; AF_1965.
GeneIDi24795708.
KEGGiafu:AF_1965.

Phylogenomic databases

eggNOGiarCOG00919. Archaea.
COG1591. LUCA.
KOiK03552.
OMAiPVLAVKF.

Miscellaneous databases

EvolutionaryTraceiO28314.

Family and domain databases

CDDicd00523. archeal_HJR. 1 hit.
Gene3Di3.40.1350.10. 1 hit.
HAMAPiMF_01490. HJ_Resolv_Hjc. 1 hit.
InterProiIPR002732. Hjc.
IPR014428. Hjc_arc.
IPR011335. Restrct_endonuc-II-like.
IPR011856. tRNA_endonuc-like_dom.
[Graphical view]
PfamiPF01870. Hjc. 1 hit.
[Graphical view]
PIRSFiPIRSF004985. Hlld_jn_rslvs_ar. 1 hit.
ProDomiPD015874. PD015874. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF52980. SSF52980. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHJC_ARCFU
AccessioniPrimary (citable) accession number: O28314
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 14, 2014
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.