Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Holliday junction resolvase Hjc

Gene

hjc

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination. Cleaves 4-way DNA junctions introducing paired nicks in opposing strands, leaving a 5'-terminal phosphate and a 3'-terminal hydroxyl group that are ligated to produce recombinant products.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi9 – 91MagnesiumUniRule annotation
Active sitei29 – 291UniRule annotation
Metal bindingi38 – 381MagnesiumUniRule annotation
Metal bindingi51 – 511MagnesiumUniRule annotation
Sitei53 – 531Transition state stabilizerSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-2002-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Holliday junction resolvase HjcUniRule annotation (EC:3.1.22.4UniRule annotation)
Short name:
HjcUniRule annotation
Gene namesi
Name:hjcUniRule annotation
Ordered Locus Names:AF_1965
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 136136Holliday junction resolvase HjcPRO_0000429153Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi224325.AF1965.

Structurei

Secondary structure

1
136
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 1814Combined sources
Beta strandi22 – 265Combined sources
Beta strandi29 – 313Combined sources
Beta strandi32 – 343Combined sources
Beta strandi38 – 425Combined sources
Beta strandi47 – 548Combined sources
Beta strandi56 – 583Combined sources
Beta strandi60 – 634Combined sources
Helixi64 – 7714Combined sources
Beta strandi80 – 867Combined sources
Beta strandi93 – 964Combined sources
Helixi97 – 993Combined sources
Beta strandi100 – 1023Combined sources
Beta strandi104 – 1096Combined sources
Turni111 – 1133Combined sources
Helixi114 – 1163Combined sources
Helixi120 – 1256Combined sources
Helixi126 – 1283Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2WCWX-ray1.58A/B/C/D2-136[»]
2WCZX-ray1.65A/B2-136[»]
2WIWX-ray1.80A/B2-136[»]
2WIZX-ray3.30A/B2-136[»]
2WJ0X-ray3.10A/B2-136[»]
ProteinModelPortaliO28314.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO28314.

Family & Domainsi

Sequence similaritiesi

Belongs to the Holliday junction resolvase Hjc family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00919. Archaea.
COG1591. LUCA.
KOiK03552.
OMAiPVLAVKF.

Family and domain databases

Gene3Di3.40.1350.10. 1 hit.
HAMAPiMF_01490. HJ_Resolv_Hjc.
InterProiIPR002732. Hjc.
IPR014428. Hjc_arc.
IPR011335. Restrct_endonuc-II-like.
IPR011856. tRNA_endonuc-like_dom.
[Graphical view]
PfamiPF01870. Hjc. 1 hit.
[Graphical view]
PIRSFiPIRSF004985. Hlld_jn_rslvs_ar. 1 hit.
ProDomiPD015874. PD015874. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

O28314-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSKGTRFER DLLVELWKAG FAAIRVAGSG VSPFPCPDIV AGNGRTYLAI
60 70 80 90 100
EVKMRKELPL YLSADEVEQL VTFARGFGAE AYVALKLPRK KWRFFPVQML
110 120 130
ERTEKNFKID ESVYPLGLEI AEVAGKFFQE RFGEKV
Length:136
Mass (Da):15,437
Last modified:January 1, 1998 - v1
Checksum:iC59D1FF6ABDE1AC8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB89293.1.
PIRiD69495.
RefSeqiWP_010879457.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB89293; AAB89293; AF_1965.
GeneIDi24795708.
KEGGiafu:AF_1965.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB89293.1.
PIRiD69495.
RefSeqiWP_010879457.1. NC_000917.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2WCWX-ray1.58A/B/C/D2-136[»]
2WCZX-ray1.65A/B2-136[»]
2WIWX-ray1.80A/B2-136[»]
2WIZX-ray3.30A/B2-136[»]
2WJ0X-ray3.10A/B2-136[»]
ProteinModelPortaliO28314.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224325.AF1965.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB89293; AAB89293; AF_1965.
GeneIDi24795708.
KEGGiafu:AF_1965.

Phylogenomic databases

eggNOGiarCOG00919. Archaea.
COG1591. LUCA.
KOiK03552.
OMAiPVLAVKF.

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-2002-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO28314.

Family and domain databases

Gene3Di3.40.1350.10. 1 hit.
HAMAPiMF_01490. HJ_Resolv_Hjc.
InterProiIPR002732. Hjc.
IPR014428. Hjc_arc.
IPR011335. Restrct_endonuc-II-like.
IPR011856. tRNA_endonuc-like_dom.
[Graphical view]
PfamiPF01870. Hjc. 1 hit.
[Graphical view]
PIRSFiPIRSF004985. Hlld_jn_rslvs_ar. 1 hit.
ProDomiPD015874. PD015874. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF52980. SSF52980. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus."
    Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G.
    , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C.
    Nature 390:364-370(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.
  2. "Characterization of crystals of the Hjc resolvase from Archaeoglobus fulgidus grown in gel by counter-diffusion."
    Biertumpfel C., Basquin J., Birkenbihl R.P., Suck D., Sauter C.
    Acta Crystallogr. F 61:684-687(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY CRYSTALLIZATION.
  3. "1.6 A resolution structure of Archaeoglobus fulgidus Hjc, a Holliday junction resolvase from an archaeal hyperthermophile."
    Carolis C., Koehler C., Sauter C., Basquin J., Suck D., Toeroe I.
    Submitted (MAR-2009) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.58 ANGSTROMS) OF 2-136.
  4. "Crystal structures of Holliday junction resolvases from Archaeoglobus fulgidus bound to DNA substrate."
    Carolis C., Koehler C., Sauter C., Basquin J., Suck D., Toeroe I.
    Submitted (MAY-2009) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (3.10 ANGSTROMS) OF 2-136.

Entry informationi

Entry nameiHJC_ARCFU
AccessioniPrimary (citable) accession number: O28314
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 14, 2014
Last sequence update: January 1, 1998
Last modified: November 11, 2015
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.