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O28303

- PAN_ARCFU

UniProt

O28303 - PAN_ARCFU

Protein

Proteasome-activating nucleotidase

Gene

pan

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    ATPase which is responsible for recognizing, binding, unfolding and translocation of substrate proteins into the archaeal 20S proteasome core particle. Is essential for opening the gate of the 20S proteasome via an interaction with its C-terminus, thereby allowing substrate entry and access to the site of proteolysis. Thus, the C-termini of the proteasomal ATPase function like a 'key in a lock' to induce gate opening and therefore regulate proteolysis. Unfolding activity requires energy from ATP hydrolysis, whereas ATP binding alone promotes ATPase-20S proteasome association which triggers gate opening, and supports translocation of unfolded substrates Probable.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei324 – 3241ATPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi185 – 1906ATPUniRule annotation

    GO - Molecular functioni

    1. ATPase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. proteasomal protein catabolic process Source: UniProtKB-HAMAP
    2. protein unfolding Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Chaperone

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciAFUL224325:GJBC-2013-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proteasome-activating nucleotidaseUniRule annotation
    Short name:
    PANUniRule annotation
    Alternative name(s):
    Proteasomal ATPaseUniRule annotation
    Proteasome regulatory ATPaseUniRule annotation
    Proteasome regulatory particleUniRule annotation
    Gene namesi
    Name:panUniRule annotation
    Ordered Locus Names:AF_1976
    OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
    Taxonomic identifieri224325 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
    ProteomesiUP000002199: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. proteasome-activating nucleotidase complex Source: UniProtKB-HAMAP

    Keywords - Cellular componenti

    Cytoplasm, Proteasome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 398398Proteasome-activating nucleotidasePRO_0000084739Add
    BLAST

    Proteomic databases

    PRIDEiO28303.

    Interactioni

    Subunit structurei

    Homohexamer. The hexameric complex has a two-ring architecture resembling a top hat that caps the 20S proteasome core at one or both ends. Upon ATP-binding, the C-terminus of PAN interacts with the alpha-rings of the proteasome core by binding to the intersubunit pockets Probable.1 Publication

    Protein-protein interaction databases

    STRINGi224325.AF1976.

    Structurei

    Secondary structure

    1
    398
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi63 – 719
    Beta strandi77 – 815
    Beta strandi86 – 894
    Turni97 – 993
    Beta strandi105 – 1095
    Turni110 – 1123
    Beta strandi115 – 1195

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2WG5X-ray2.10A/B/C/D/E/F/G/H/I/J/K/L57-134[»]
    2WG6X-ray2.50A/B/C/D/E/F/G/H/I/J/K/L57-134[»]
    ProteinModelPortaliO28303.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO28303.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni396 – 3983Docks into pockets in the proteasome alpha-ring to cause gate openingUniRule annotation

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili3 – 6058UniRule annotationAdd
    BLAST

    Domaini

    Consists of three main regions, an N-terminal coiled-coil domain that may assist in substrate recognition, an interdomain involved in PAN hexamerization, and a C-terminal ATPase domain of the AAA type.UniRule annotation

    Sequence similaritiesi

    Belongs to the AAA ATPase family.UniRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG1222.
    KOiK03420.
    OMAiEREVNRT.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    HAMAPiMF_00553. PAN.
    InterProiIPR005937. 26S_Psome_P45.
    IPR003593. AAA+_ATPase.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR023501. Nucleotidase_PAN.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00004. AAA. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR01242. 26Sp45. 1 hit.
    PROSITEiPS00674. AAA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O28303-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGDSEIQYLL EKLKKLEEDY YKLRELYRRL EDEKKFIESE RIRYEREVRR    50
    LRSEVERLRS PPLLVGVVSD ILEDGRVVVK SSTGPKFVVN TSQYINEEEL 100
    KPGARVALNQ QTLAIVNVLP TSKDPMVYGF EVEEKPEVSY EDIGGLDVQI 150
    EEIREAVELP LLKPELFAEV GIEPPKGVLL YGPPGTGKTL LAKAVANQTR 200
    ATFIRVVGSE FVQKYIGEGA RLVREVFQLA KEKAPSIIFI DELDAIAARR 250
    TNSDTSGDRE VQRTMMQLLA ELDGFDPRGD VKVIGATNRI DILDPAILRP 300
    GRFDRIIEVP LPTFEGRIQI FKIHTRKMKL AEDVDFKELA RITEGASGAD 350
    IKAICTEAGM FAIREERAKV TMLDFTKAIE KVLKKTTPIP DLKGVMFV 398
    Length:398
    Mass (Da):44,964
    Last modified:January 1, 1998 - v1
    Checksum:iF3293BB7D6A646B4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000782 Genomic DNA. Translation: AAB89280.1.
    PIRiG69496.
    RefSeqiNP_070800.1. NC_000917.1.
    WP_010879468.1. NC_000917.1.

    Genome annotation databases

    EnsemblBacteriaiAAB89280; AAB89280; AF_1976.
    GeneIDi1485198.
    KEGGiafu:AF1976.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000782 Genomic DNA. Translation: AAB89280.1 .
    PIRi G69496.
    RefSeqi NP_070800.1. NC_000917.1.
    WP_010879468.1. NC_000917.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2WG5 X-ray 2.10 A/B/C/D/E/F/G/H/I/J/K/L 57-134 [» ]
    2WG6 X-ray 2.50 A/B/C/D/E/F/G/H/I/J/K/L 57-134 [» ]
    ProteinModelPortali O28303.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224325.AF1976.

    Proteomic databases

    PRIDEi O28303.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB89280 ; AAB89280 ; AF_1976 .
    GeneIDi 1485198.
    KEGGi afu:AF1976.

    Phylogenomic databases

    eggNOGi COG1222.
    KOi K03420.
    OMAi EREVNRT.

    Enzyme and pathway databases

    BioCyci AFUL224325:GJBC-2013-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei O28303.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    HAMAPi MF_00553. PAN.
    InterProi IPR005937. 26S_Psome_P45.
    IPR003593. AAA+_ATPase.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR023501. Nucleotidase_PAN.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00004. AAA. 1 hit.
    [Graphical view ]
    SMARTi SM00382. AAA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR01242. 26Sp45. 1 hit.
    PROSITEi PS00674. AAA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus."
      Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G.
      , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C.
      Nature 390:364-370(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.
    2. "Structure and activity of the N-terminal substrate recognition domains in proteasomal ATPases."
      Djuranovic S., Hartmann M.D., Habeck M., Ursinus A., Zwickl P., Martin J., Lupas A.N., Zeth K.
      Mol. Cell 34:580-590(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 57-134 OF WILD-TYPE AND MUTANT ALA-61, FUNCTION AS A CHAPERONE, SUBUNIT.

    Entry informationi

    Entry nameiPAN_ARCFU
    AccessioniPrimary (citable) accession number: O28303
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3