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O28303

- PAN_ARCFU

UniProt

O28303 - PAN_ARCFU

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Protein

Proteasome-activating nucleotidase

Gene

pan

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

ATPase which is responsible for recognizing, binding, unfolding and translocation of substrate proteins into the archaeal 20S proteasome core particle. Is essential for opening the gate of the 20S proteasome via an interaction with its C-terminus, thereby allowing substrate entry and access to the site of proteolysis. Thus, the C-termini of the proteasomal ATPase function like a 'key in a lock' to induce gate opening and therefore regulate proteolysis. Unfolding activity requires energy from ATP hydrolysis, whereas ATP binding alone promotes ATPase-20S proteasome association which triggers gate opening, and supports translocation of unfolded substrates (Probable).1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei324 – 3241ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi185 – 1906ATPUniRule annotation

GO - Molecular functioni

  1. ATPase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. proteasomal protein catabolic process Source: UniProtKB-HAMAP
  2. protein unfolding Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-2013-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome-activating nucleotidaseUniRule annotation
Short name:
PANUniRule annotation
Alternative name(s):
Proteasomal ATPaseUniRule annotation
Proteasome regulatory ATPaseUniRule annotation
Proteasome regulatory particleUniRule annotation
Gene namesi
Name:panUniRule annotation
Ordered Locus Names:AF_1976
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
ProteomesiUP000002199: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
  2. proteasome-activating nucleotidase complex Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 398398Proteasome-activating nucleotidasePRO_0000084739Add
BLAST

Proteomic databases

PRIDEiO28303.

Interactioni

Subunit structurei

Homohexamer. The hexameric complex has a two-ring architecture resembling a top hat that caps the 20S proteasome core at one or both ends. Upon ATP-binding, the C-terminus of PAN interacts with the alpha-rings of the proteasome core by binding to the intersubunit pockets (Probable).1 Publication

Protein-protein interaction databases

STRINGi224325.AF1976.

Structurei

Secondary structure

1
398
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi63 – 719Combined sources
Beta strandi77 – 815Combined sources
Beta strandi86 – 894Combined sources
Turni97 – 993Combined sources
Beta strandi105 – 1095Combined sources
Turni110 – 1123Combined sources
Beta strandi115 – 1195Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2WG5X-ray2.10A/B/C/D/E/F/G/H/I/J/K/L57-134[»]
2WG6X-ray2.50A/B/C/D/E/F/G/H/I/J/K/L57-134[»]
ProteinModelPortaliO28303.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO28303.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni396 – 3983Docks into pockets in the proteasome alpha-ring to cause gate openingUniRule annotation

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili3 – 6058UniRule annotationAdd
BLAST

Domaini

Consists of three main regions, an N-terminal coiled-coil domain that may assist in substrate recognition, an interdomain involved in PAN hexamerization, and a C-terminal ATPase domain of the AAA type.UniRule annotation

Sequence similaritiesi

Belongs to the AAA ATPase family.UniRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG1222.
KOiK03420.
OMAiEREVNRT.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00553. PAN.
InterProiIPR005937. 26S_Psome_P45.
IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR023501. Nucleotidase_PAN.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01242. 26Sp45. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O28303-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGDSEIQYLL EKLKKLEEDY YKLRELYRRL EDEKKFIESE RIRYEREVRR
60 70 80 90 100
LRSEVERLRS PPLLVGVVSD ILEDGRVVVK SSTGPKFVVN TSQYINEEEL
110 120 130 140 150
KPGARVALNQ QTLAIVNVLP TSKDPMVYGF EVEEKPEVSY EDIGGLDVQI
160 170 180 190 200
EEIREAVELP LLKPELFAEV GIEPPKGVLL YGPPGTGKTL LAKAVANQTR
210 220 230 240 250
ATFIRVVGSE FVQKYIGEGA RLVREVFQLA KEKAPSIIFI DELDAIAARR
260 270 280 290 300
TNSDTSGDRE VQRTMMQLLA ELDGFDPRGD VKVIGATNRI DILDPAILRP
310 320 330 340 350
GRFDRIIEVP LPTFEGRIQI FKIHTRKMKL AEDVDFKELA RITEGASGAD
360 370 380 390
IKAICTEAGM FAIREERAKV TMLDFTKAIE KVLKKTTPIP DLKGVMFV
Length:398
Mass (Da):44,964
Last modified:January 1, 1998 - v1
Checksum:iF3293BB7D6A646B4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB89280.1.
PIRiG69496.
RefSeqiNP_070800.1. NC_000917.1.
WP_010879468.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB89280; AAB89280; AF_1976.
GeneIDi1485198.
KEGGiafu:AF1976.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB89280.1 .
PIRi G69496.
RefSeqi NP_070800.1. NC_000917.1.
WP_010879468.1. NC_000917.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2WG5 X-ray 2.10 A/B/C/D/E/F/G/H/I/J/K/L 57-134 [» ]
2WG6 X-ray 2.50 A/B/C/D/E/F/G/H/I/J/K/L 57-134 [» ]
ProteinModelPortali O28303.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224325.AF1976.

Proteomic databases

PRIDEi O28303.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB89280 ; AAB89280 ; AF_1976 .
GeneIDi 1485198.
KEGGi afu:AF1976.

Phylogenomic databases

eggNOGi COG1222.
KOi K03420.
OMAi EREVNRT.

Enzyme and pathway databases

BioCyci AFUL224325:GJBC-2013-MONOMER.

Miscellaneous databases

EvolutionaryTracei O28303.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
HAMAPi MF_00553. PAN.
InterProi IPR005937. 26S_Psome_P45.
IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR023501. Nucleotidase_PAN.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00004. AAA. 1 hit.
[Graphical view ]
SMARTi SM00382. AAA. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR01242. 26Sp45. 1 hit.
PROSITEi PS00674. AAA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus."
    Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G.
    , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C.
    Nature 390:364-370(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.
  2. "Structure and activity of the N-terminal substrate recognition domains in proteasomal ATPases."
    Djuranovic S., Hartmann M.D., Habeck M., Ursinus A., Zwickl P., Martin J., Lupas A.N., Zeth K.
    Mol. Cell 34:580-590(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 57-134 OF WILD-TYPE AND MUTANT ALA-61, FUNCTION AS A CHAPERONE, SUBUNIT.

Entry informationi

Entry nameiPAN_ARCFU
AccessioniPrimary (citable) accession number: O28303
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: November 26, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3