Reviewed, UniProtKB/Swiss-Prot O28279 (SAHH_ARCFU)

Names and origin

Protein names

Recommended name:
    Adenosylhomocysteinase
    EC=3.3.1.1
Alternative name(s):
    S-adenosyl-L-homocysteine hydrolase
      Short name=AdoHcyase

Gene names

Name:	ahcY
Ordered Locus Names:	AF_2000

Organism

Archaeoglobus fulgidus [Complete proteome] [HAMAP]

Taxonomic identifier

2234 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	405 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine. HAMAP MF_00563
Cofactor	Binds 1 NAD per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. HAMAP MF_00563
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the adenosylhomocysteinase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	adenosylhomocysteinase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 405	405	Adenosylhomocysteinase HAMAP MF_00563		PRO_0000117001
Regions
	Region	165 – 331	167	NAD binding By similarity
Sites
	Binding site	113	1	Substrate By similarity
	Binding site	138	1	Substrate By similarity
	Binding site	168	1	Substrate By similarity
	Binding site	172	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O28279-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 9B240D3E3E5C17C4	FASTA	405	45,395
	10 20 30 40 50 60 MEGFRKIEWA ERYMKVLGKI REQFRKERPL EGFTVGMALH VEAKTAVLVR TLVDAGAEVA 70 80 90 100 110 120 ITGCNPMSTQ DDVADALRES GIACYAKRGM DVEEYYEALR NVIRAEPDIV IDDGADLIFL 130 140 150 160 170 180 LHGEMESYAE KVKGASEETT TGVIRLRAME REGVLKFPVI AVNDAYTKYL FDNRYGTGQS 190 200 210 220 230 240 AIDGVIRATN LLMAGKIVVV AGYGWCGRGI AMRARGMGAS VVVTEVDEIR ALEAVMDGFR 250 260 270 280 290 300 VMRMEDAAKI GDIFITATGN RDIIREEHIR LMKDGAILAN AGHFNVEIDI PALERMAKAK 310 320 330 340 350 360 REARKYVTEY DLGDKRVYLL AEGRLVNLVA ADGHPVEVMD MSFANQALAA KYIAENWQKL 370 380 390 400 ERKVYRLPEE LDRMVARMKL ESMGVEIDQL TEEQVRYLSD WRCGT

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000782 Genomic DNA. Translation: AAB89252.1.
PIR	G69499.
RefSeq	NP_070824.1.
3D structure databases
HSSP	HSSP built from PDB template 1LI4 based on UniProtKB P23526.
ModBase	Search...
Genome annotation databases
GeneID	1485225.
GenomeReviews	Gene locus AF_2000 in contig AE000782_GR.
KEGG	afu:AF2000.
NMPDR	fig|224325.1.peg.1985.
TIGR	AF_2000.
Phylogenomic databases
HOGENOM	O28279.
OMA	IRLKAMA.
Enzyme and pathway databases
BioCyc	AFUL224325:AF_2000-MON.
BRENDA	3.3.1.1. 7576.
Family and domain databases
HAMAP	MF_00563. [Tree]
InterPro	IPR015878. Ado_hCys_hydrolase_NAD-bd. IPR000043. S-Ado-L-homoCys_hydrolase. IPR020082. S-Ado-L-homoCys_hydrolase_CS. [Graphical view]
PANTHER	PTHR23420. Ad_hcy_hydrolase. 1 hit.
Pfam	PF05221. AdoHcyase. 1 hit. PF00670. AdoHcyase_NAD. 1 hit. [Graphical view]
PIRSF	PIRSF001109. Ad_hcy_hydrolase. 1 hit.
TIGRFAMs	TIGR00936. ahcY. 1 hit.
PROSITE	PS00738. ADOHCYASE_1. 1 hit. PS00739. ADOHCYASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAHH_ARCFU

Accession

Primary (citable) accession number: O28279

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	November 3, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)