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Protein

Uridylate kinase

Gene

pyrH

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible phosphorylation of UMP to UDP.UniRule annotation

Catalytic activityi

ATP + UMP = ADP + UDP.UniRule annotation

Enzyme regulationi

Inhibited by UTP.UniRule annotation

Pathwayi: CTP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes UDP from UMP (UMPK route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Uridylate kinase (pyrH)
This subpathway is part of the pathway CTP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UDP from UMP (UMPK route), the pathway CTP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei41 – 411UMP; via amide nitrogenUniRule annotation
Binding sitei42 – 421ATP; via amide nitrogenUniRule annotation
Binding sitei46 – 461ATPUniRule annotation
Binding sitei63 – 631UMPUniRule annotation
Binding sitei136 – 1361ATPUniRule annotation
Binding sitei137 – 1371ATPUniRule annotation
Binding sitei142 – 1421ATP; via amide nitrogen and carbonyl oxygenUniRule annotation
Binding sitei145 – 1451ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 102ATPUniRule annotation
Nucleotide bindingi110 – 1167UMPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-2084-MONOMER.
UniPathwayiUPA00159; UER00275.

Names & Taxonomyi

Protein namesi
Recommended name:
Uridylate kinaseUniRule annotation (EC:2.7.4.22UniRule annotation)
Short name:
UKUniRule annotation
Alternative name(s):
Uridine monophosphate kinaseUniRule annotation
Short name:
UMP kinaseUniRule annotation
Short name:
UMPKUniRule annotation
Gene namesi
Name:pyrHUniRule annotation
Ordered Locus Names:AF_2042
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 219219Uridylate kinasePRO_0000143914Add
BLAST

Interactioni

Subunit structurei

Homohexamer.UniRule annotation

Protein-protein interaction databases

STRINGi224325.AF2042.

Structurei

Secondary structure

1
219
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 76Combined sources
Turni11 – 144Combined sources
Helixi16 – 3217Combined sources
Beta strandi33 – 397Combined sources
Helixi42 – 5413Combined sources
Helixi59 – 8022Combined sources
Helixi92 – 998Combined sources
Beta strandi103 – 1075Combined sources
Beta strandi111 – 1144Combined sources
Helixi117 – 12610Combined sources
Beta strandi130 – 14011Combined sources
Beta strandi147 – 1515Combined sources
Beta strandi156 – 1583Combined sources
Helixi160 – 1667Combined sources
Helixi182 – 19110Combined sources
Beta strandi195 – 1995Combined sources
Helixi202 – 2098Combined sources
Beta strandi215 – 2184Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2IJ9X-ray2.90A/B1-219[»]
ProteinModelPortaliO28237.
SMRiO28237. Positions 1-219.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO28237.

Family & Domainsi

Sequence similaritiesi

Belongs to the UMP kinase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00858. Archaea.
COG0528. LUCA.
KOiK09903.
OMAiGIHITRA.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
HAMAPiMF_01220_A. PyrH_A.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR011817. Uridylate_kinase.
IPR011818. Uridylate_kinase_arch/spir.
[Graphical view]
PANTHERiPTHR21499:SF23. PTHR21499:SF23. 1 hit.
PfamiPF00696. AA_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF005650. Uridylate_kin. 1 hit.
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR02076. pyrH_arch. 1 hit.

Sequencei

Sequence statusi: Complete.

O28237-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVVLSLGGS VLSNESEKIR EFAKTIESVA QQNQVFVVVG GGKLAREYIK
60 70 80 90 100
RARELGASET FCDYIGIAAT RLNAMLLISA IPSAAKKVPV DFMEAEELSK
110 120 130 140 150
LYRVVVMGGT FPGHTTDATA ALLAEFIKAD VFINATNVDG VYSADPKSDT
160 170 180 190 200
SAVKYDRLSP QQLVEIVSRS SAKAGTNVVI DLLAAKIIER SKIKTYVILG
210
TPENIMKAVK GEAVGTVIA
Length:219
Mass (Da):23,398
Last modified:January 1, 1998 - v1
Checksum:i751B894ED5261B91
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB89213.1.
PIRiA69505.
RefSeqiWP_010879534.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB89213; AAB89213; AF_2042.
GeneIDi24795791.
KEGGiafu:AF_2042.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB89213.1.
PIRiA69505.
RefSeqiWP_010879534.1. NC_000917.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2IJ9X-ray2.90A/B1-219[»]
ProteinModelPortaliO28237.
SMRiO28237. Positions 1-219.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224325.AF2042.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB89213; AAB89213; AF_2042.
GeneIDi24795791.
KEGGiafu:AF_2042.

Phylogenomic databases

eggNOGiarCOG00858. Archaea.
COG0528. LUCA.
KOiK09903.
OMAiGIHITRA.

Enzyme and pathway databases

UniPathwayiUPA00159; UER00275.
BioCyciAFUL224325:GJBC-2084-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO28237.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
HAMAPiMF_01220_A. PyrH_A.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR011817. Uridylate_kinase.
IPR011818. Uridylate_kinase_arch/spir.
[Graphical view]
PANTHERiPTHR21499:SF23. PTHR21499:SF23. 1 hit.
PfamiPF00696. AA_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF005650. Uridylate_kin. 1 hit.
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR02076. pyrH_arch. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus."
    Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G.
    , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C.
    Nature 390:364-370(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.
  2. "Crystal structure of uridylate kinase from Archaeoglobus fulgidus."
    New York structural genomics research consortium (NYSGRC)
    Submitted (OCT-2006) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS).

Entry informationi

Entry nameiPYRH_ARCFU
AccessioniPrimary (citable) accession number: O28237
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: January 1, 1998
Last modified: November 11, 2015
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.